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MB Sample ID: SA155494
Local Sample ID: | CD-PBS_3.1.4 |
Subject ID: | SU001764 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57Bl/6N |
Age Or Age Range: | 35 weeks |
Gender: | Male |
Animal Animal Supplier: | KU Leuven Animal Laboratory |
Animal Feed: | control diet (E15742-33 ssniff Spezialdiäten GmbH) or 60% high fat diet (S8655-E220 sniff Spezialdiäten GmbH) |
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Sample Preparation:
Sampleprep ID: | SP001770 |
Sampleprep Summary: | Lipids were extracted from liver samples with 700 μl of sample (100 μl of plasma diluted in PBS, or 700 μl of homogenized cells) was mixed with 800 μl 1 N HCl:CH3OH 1:8 (v/v), 900 μl CHCl3, 200 μg/ml of the antioxidant 2,6-di-tert-butyl-4-methylphenol (BHT; Sigma Aldrich) and 3 μl of SPLASH® LIPIDOMIX® Mass Spec Standard (#330707, Avanti Polar Lipids). After vortexing and centrifugation, the lower organic fraction was collected and evaporated using a Savant Speedvac spd111v (Thermo Fisher Scientific) at room temperature and the remaining lipid pellet was stored at - 20°C under argon. Just before mass spectrometry analysis, lipid pellets were reconstituted in 100% ethanol. |