Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA318294

Local Sample ID:	Sample 10
Subject ID:	SU003046
Subject Type:	Cultured cells
Subject Species:	Symbiodiniaceae

Select appropriate tab below to view additional metadata details:

All
Subject
Factors
Collection
Treatment
Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP003052
Sampleprep Summary:	Seven days after the antibiotic treatment process, 3 × 1 mL aliquots were collected from each culture flask for flow cytometry cell counting of both bacteria and Symbiodiniaceae cells, bacteria community analysis via 16S rRNA amplicon sequencing analysis, and Symbiodiniaceae photophysiological measurements (methods described in more detail below). The remaining Symbiodiniaceae cells were concentrated by centrifugation at 700 × g for 10 mins at 26˚C, the media discarded, and Symbiodiniaceae pellets snap frozen in liquid nitrogen for metabolite profiling.

Sampleprep ID:

SP003052

Sampleprep Summary:

Seven days after the antibiotic treatment process, 3 × 1 mL aliquots were collected from each culture flask for flow cytometry cell counting of both bacteria and Symbiodiniaceae cells, bacteria community analysis via 16S rRNA amplicon sequencing analysis, and Symbiodiniaceae photophysiological measurements (methods described in more detail below). The remaining Symbiodiniaceae cells were concentrated by centrifugation at 700 × g for 10 mins at 26˚C, the media discarded, and Symbiodiniaceae pellets snap frozen in liquid nitrogen for metabolite profiling.