Summary of Study ST000387
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000303. The data can be accessed directly via it's Project DOI: 10.21228/M8W593 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000387 |
| Study Title | Changes in the metabalome and lipidome in response to exercise training |
| Study Type | HERITAGE (HEalth, RIsk factors, exercise Training And GEnetics) family study |
| Study Summary | The overall objective of the Heritage Family Study is to study the role of the genotype in cardiovascular, metabolic, and hormonal responses to aerobic exercise training and the contribution of regular exercise to changes in several cardiovascular disease and diabetes risk factors. The study cohort used in this analysis was derived from the pool of 473 Caucasian subjects (230 male and 243 female) from 99 nuclear families who completed ≥58 of the prescribed 60 exercise-training sessions. Utilizing a subsample of this Caucasian cohort, we selected family members from the Quebec center (N=125) to assess the metabolome and lipidome of circulating plasma under two well-defined environmental conditions, the pre- and post-training conditions. |
| Institute | University of California, Davis |
| Department | Genome and Biomedical Sciences Facility |
| Laboratory | WCMC Metabolomics Core |
| Last Name | Fiehn |
| First Name | Oliver |
| Address | Health Sciences Drive, Davis, California, 95616, USA |
| ofiehn@ucdavis.edu | |
| Phone | (530) 754-8258 |
| Submit Date | 2016-04-26 |
| Num Groups | 3 |
| Total Subjects | 250 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-04-29 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP000415 |
| Sampleprep Summary: | 1) Degas both “MeOH with QCmix” and MTBE with Cholesterol Ester 22:1 bysonication 2) Store solvents in the –20°C freezer to pre-chill 3) Thaw each 20 μL plasma aliquot at room temperature. Once thawed (~10min) place liquid plasma samples on ice. 4) Add 225 μL cold “MeOH with QC mix” (see SOP “QC mix for LC-MS lipid analysis”). Keep MeOH on ice during extraction 5) Vortex each sample for 10s, keeping the rest on ice during all the extraction. 6) Add 750 μL of cold MTBE with 22:1 CE, keep MTBE on ice during extraction Vortex for 10s 7) Shake for 6min at 4°C in the orbital mixer. Add 188 μL room temperature LC/MS grade water. 8)Vortex for 20 s 9) Centrifuge for 2 min @ 14,000 rcf (12300 rpm) 10) Remove supernatant, splitting into two aliquots of 350 μL, keeping one at –20°C for backup 11) Dry samples to complete dryness in the speed vacuum concentration system |
| Sampleprep Protocol Filename: | SOP_Lipid_Extraction_Plasma_06242013 (1).pdf |
| Processing Method: | Homogenization |
