Summary of Study ST003149
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001958. The data can be accessed directly via it's Project DOI: 10.21228/M8MX5P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003149 |
Study Title | Impact of early-life exposure to a potent aryl hydrocarbon receptor ligand on gut microbiota and host glucose homeostasis in C57BL/6J male mice (Part I) |
Study Summary | This study aimed to explore the association between early-life exposure to a potent aryl hydrocarbon receptor (AHR) agonist and persistent disruptions in the microbiota, leading to impaired metabolic homeostasis later in life. This study utilized metagenomics, NMR- and mass spectrometry-based metabolomics, and biochemical assays to analyze the gut microbiome composition and function, as well as the physiological and metabolic effects of early-life exposure to 2,3,7,8-tetrachlorodibenzofuran (TCDF) in conventional, germ-free (GF), and Ahr-null mice. The impact of TCDF on Akkermansia muciniphila (A. muciniphila) in vitro was assessed using optical density (OD 600), flow cytometry, transcriptomics, and mass spectrometry-based metabolomics. TCDF-exposed mice exhibited disruption in the gut microbiome community structure and function, characterized by lower abundances of A. muciniphila, lower levels of cecal short chain fatty acids (SCFAs) and indole-3-lactic acid (ILA), and a reduction in gut hormones GLP-1 and PYY. Importantly, microbial and metabolic phenotypes associated with early-life POP exposure were transferable to GF recipients in the absence of POP carry-over. In addition, AHR-independent interactions between POPs and the microbiota were observed, significantly affected the growth, physiology, gene expression, and metabolic activity of A. muciniphila, resulting in suppressed activity along the ILA pathway. |
Institute | Pennsylvania State University |
Department | Department of Veterinary and Biomedical Sciences |
Last Name | Koo |
First Name | Imhoi |
Address | 307B Life Science Building |
iuk41@psu.edu | |
Phone | 8148107425 |
Submit Date | 2024-03-28 |
Raw Data Available | Yes |
Raw Data File Type(s) | fid |
Analysis Type Detail | NMR |
Release Date | 2024-04-30 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP003273 |
Sampleprep Summary: | For preparation of urine samples, 550 μL urine was blended with 55 μL of phosphate buffer (K2HPO4/NaH2PO4, 1.5 M, pH 7.4, 100% D2O) containing 0.01% TSP for chemical shift reference (δ0.00). After centrifugation (11 180 ×g, 4 °C) for 10 min, 550 μL supernatant was transferred into 5 mm NMR tubes for NMR analysis. Each liver tissue sample (55 ± 5 mg) was extracted three times with 600 μL of methanol/H2O (2:1) using a Qiagen tissue-lyzer. Three resultant supernatants were combined and subjected to centrifugation (16 099 ×g, 4 °C, 10 min). After removing methanol in vacuo, the resultant supernatant was lyophilized, weighed, and reconstituted into 600 μL phosphate buffer (0.1 M, K2HPO4/NaH2PO4, pH 7.4) containing 0.005% TSP, 50% D2O and 0.01% NaN3. After final centrifugation (16 099 ×g, 4 °C, 10 min), each supernatant (550 μL) was transferred into a 5 mm NMR tube for NMR analysis. Each fecal sample (55 ± 5 mg) were extracted twice with 500 μL phosphate buffer (0.1 M, K2HPO4/NaH2PO4, pH 7.4) containing 0.005% TSP, 30% D2O and 0.01% NaN3. After centrifugation (16 099 ×g, 4 °C, 10 min), 600 μL combined supernatants were transferred into 5 mm NMR tubes for NMR analysis. For liver lipid quantification, 50 mg of liver tissue was homogenized in 1 mL pre-cooled chloroform/methanol mix [1:1 (v/v)], followed by adding 296 µL water. After centrifugation (10,000 g, 4°C) for 10 min, the lower phase was dried in a vacuum and reconstituted in 600 µL of deuterated chloroform containing 0.03% (v/v) tetramethylsilane (TMS). |