Summary of Study ST002437
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001569. The data can be accessed directly via it's Project DOI: 10.21228/M8WD9H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002437 |
| Study Title | Metabolomic analysis in wildtype and Isg15 knockout mice after sham or transverse aortic constriction surgery |
| Study Summary | Left ventricular tissues were isolated from wildtype and Isg15 knockout mice 8 weeks after sham or transverse aortic constriction and the metabolomic differences were compared. |
| Institute | St. Michael's Hospital |
| Last Name | Advani |
| First Name | Andrew |
| Address | 6-151, 61 Queen Street East, Toronto, Ontario. M5C 2t2 |
| andrew.advani@unityhealth.to | |
| Phone | 4163604000 |
| Submit Date | 2023-01-07 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-07-26 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002532 |
| Sampleprep Summary: | 200 uL of each sample supernatants were diluted with 200 uL H2O and 100 uL used for the LC-MS analysis. LC-MS analyses were performed using Vanquish UPLC with 0-100% 10 minutes gradient (0.1% FA - 0.1%FA/MeCN) at 200 uL/min flow rate on Phenomenex Luna Omega 3 um Polar C18 100A 50x2.1 mm (RP-LC-MS). 10 uL injection volumes were used for each run. Mass spectra were acquired using Thermo Orbitrap QE+ spectrometer in positive and negative modes in separate runs. |
