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MB Sample ID: SA011151
Local Sample ID: | KC2 |
Subject ID: | SU000261 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Biosource Or Supplier: | ATCC |
Cell Strain Details: | HepG2 |
Subject Comments: | NA |
Cell Primary Immortalized: | Immortalized |
Cell Passage Number: | NA |
Cell Counts: | NA |
Species Group: | Human |
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Treatment:
Treatment ID: | TR000272 |
Treatment Summary: | Cells treated 24hr with 500 µM Fa, analogue or BSA control. Harvested and lipids extracted and then resolved using Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FTICR-MS) s |
Treatment Protocol ID: | CBC_treatment |
Treatment Protocol Filename: | See_Comments |
Treatment Protocol Comments: | Human hepatoma HepG2 cells (American Type Culture Collection; HB-8065) were grown in 75 ml tissue cell culture flasks in Eagle's minimal essential medium (EMEM) augmented with 10% fetal bovine serum at 37°C in a humidified atmosphere with 5% CO2. For treatment with fatty acids or analogues, the cells were seeded at a density of 2 × 106 cells in a T25-cm2 flask for 24 h. Each FA or analogue was added to the culture medium as a fatty acid-bovine serum albumin (BSA) complex (2.5:1, FA:BSA) to give 500µM final concentration. The controls in these experiments were HepG2 cells with BSA alone. After 24 h treatment, the media was collected, cells were rinsed twice with PBS and cells were harvested for complex lipid analysis. |
Treatment Compound: | Fatty acid/BSA |
Treatment Dose: | 500 µM (fatty acid) |
Treatment Doseduration: | 24 hr |
Treatment Vehicle: | PBS |
Cell Growth Container: | 75 ml tissue cell culture flasks |
Cell Media: | Eagle's minimal essential medium (EMEM) augmented with 10% fetal bovine serum |
Cell Envir Cond: | 37°C in a humidified atmosphere with 5% CO2 |
Cell Harvesting: | Typsinize and scrape |
Cell Media Lastchanged: | 24 hr |