Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA090803

Local Sample ID:	604
Subject ID:	SU001313
Subject Type:	Mammal
Subject Species:	Bos taurus
Taxonomy ID:	9913
Gender:	Female

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Treatment:

Treatment ID:	TR001328
Treatment Summary:	For cows assigned to the day 4 group, upon observation of estrus and a dominant follicle by ultrasound, cows were given an injection of GnRH (Factrel, 100 µg; Zoetis) in order to precisely time ovulation relative to time of collection for these early CL. Cows were slaughtered on day 4 following estrus. For samples collected later than day 4, precise synchrony of ovulation relative to CL collection was not necessary, so no GnRH was given, and CL were collected via colpotomy. For CL of pregnancy, cows were bred by artificial insemination and a uterine flush was performed immediately following CL collection and was examined for embryo fragments to confirm the presence of a viable pregnancy. For all samples, tissue was snap frozen in liquid nitrogen immediately following tissue collection and stored at -80 degrees Celsius thereafter. For in vitro experiments, three to five dairy cows were used in each group, CL were collected on day 10-12 of the estrous cycle, and each treatment was applied to cells from each cow

Treatment ID:

TR001328

Treatment Summary:

For cows assigned to the day 4 group, upon observation of estrus and a dominant follicle by ultrasound, cows were given an injection of GnRH (Factrel, 100 µg; Zoetis) in order to precisely time ovulation relative to time of collection for these early CL. Cows were slaughtered on day 4 following estrus. For samples collected later than day 4, precise synchrony of ovulation relative to CL collection was not necessary, so no GnRH was given, and CL were collected via colpotomy. For CL of pregnancy, cows were bred by artificial insemination and a uterine flush was performed immediately following CL collection and was examined for embryo fragments to confirm the presence of a viable pregnancy. For all samples, tissue was snap frozen in liquid nitrogen immediately following tissue collection and stored at -80 degrees Celsius thereafter. For in vitro experiments, three to five dairy cows were used in each group, CL were collected on day 10-12 of the estrous cycle, and each treatment was applied to cells from each cow