Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA274780

Local Sample ID:	3''-02-8406
Subject ID:	SU002837
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Male

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Treatment:

Treatment ID:	TR002846
Treatment Summary:	Three biological replicates were prepared for each treatment condition in T75 cm2 ﬂasks (MGB4 and/or Doxorubicin and control group), and each replicate was seeded with approximately 2 × 106 LNCaP cells, then separately treated with MGB4 (2.15 μM) and/or Doxorubicin (1.18 μM) and incubated for 24 h. A uniform number of cells was seeded in T75 cm2 ﬂask for each sample to avoid the effect of variable cell number on the outcome of the experiment. After incubation, cells were washed twice with phosphate-buffered saline solution (PBS) and then collected by trypsinization. Finally, cells were collected as pellets by centrifugation at 1200 rounds per minute (rpm) for 10 min at room temperature and resuspended in 1 mL 1× PBS for further analysis. Cells were maintained in the same conditions during incubation, and all samples were collected simultaneously (Figure 5).

Treatment ID:

TR002846

Treatment Summary:

Three biological replicates were prepared for each treatment condition in T75 cm2 ﬂasks (MGB4 and/or Doxorubicin and control group), and each replicate was seeded with approximately 2 × 106 LNCaP cells, then separately treated with MGB4 (2.15 μM) and/or Doxorubicin (1.18 μM) and incubated for 24 h. A uniform number of cells was seeded in T75 cm2 ﬂask for each sample to avoid the effect of variable cell number on the outcome of the experiment. After incubation, cells were washed twice with phosphate-buffered saline solution (PBS) and then collected by trypsinization. Finally, cells were collected as pellets by centrifugation at 1200 rounds per minute (rpm) for 10 min at room temperature and resuspended in 1 mL 1× PBS for further analysis. Cells were maintained in the same conditions during incubation, and all samples were collected simultaneously (Figure 5).