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MB Sample ID: SA289160
Local Sample ID: | MED1 Aqueous Sample 6 |
Subject ID: | SU002851 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Treatment:
Treatment ID: | TR002860 |
Treatment Summary: | Mouse liver metabolites were combined with the condensate-forming low-complexity domain of MED1. Condensates were stimulated with 150 nM RNA and then incubated for 10 min. In a subset of samples, RNA addition occurred 10 min before metabolite addition. Next, condensates were centrifuged to the bottom of a 600 ul tube. Equal fractions from the input sample, aqueous phase and condensate phases were collected separately. Metabolites were extracted from each fraction using 80% methanol in steps that involved disrupting condensates with heat. In a subset of samples, this heat step was omitted. |