Summary of Study ST002878
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001761. The data can be accessed directly via it's Project DOI: 10.21228/M83139 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002878 |
Study Title | Atlas of fetal metabolism during mid-to-late gestation and diabetic pregnancy. Dynamic Labelling experiment. |
Study Summary | Mounting evidence supports an instructive role for metabolism in stem cell fate decisions. However, much is yet unknown about how fetal metabolism changes during mammalian development and how altered maternal metabolism shapes fetal metabolism. Here, we present a descriptive atlas of in vivo fetal murine metabolism during mid-to-late gestation in normal and diabetic pregnancy. Using 13C-glucose and LC-MS, we profiled the metabolism of fetal brains, hearts, livers, and placentas harvested from pregnant dams between embryonic days (E)10.5 and 18.5. Comparative analysis of our large metabolomics dataset revealed metabolic features specific to fetal tissues developed under a hyperglycemic environment as well as metabolic signatures that may denote developmental transitions during euglycemic development. We observed sorbitol accumulation in fetal tissues and altered neurotransmitter levels in fetal brains isolated from dams with maternal hyperglycemia. Tracing 13C-glucose revealed disparate nutrient sourcing in fetuses depending on maternal glycemic states. Regardless of glycemic state, histidine-derived metabolites accumulated during late development in fetal tissues and maternal plasma. Our rich dataset presents a comprehensive overview of in vivo fetal tissue metabolism and alterations occurring as a result of maternal hyperglycemia. |
Institute | University of California, Los Angeles |
Department | Biological Chemistry |
Laboratory | Heather Christofk |
Last Name | Matulionis |
First Name | Nedas |
Address | 615 Charles E Young Drive South Los Angeles, CA, 90095 |
nmatulionis@mednet.ucla.edu | |
Phone | 3102060163 |
Submit Date | 2023-09-25 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2023-12-08 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR003000 |
Treatment Summary: | Pregnant mice were placed under a maintained plane of isoflurane anesthesia (2.5%). Retro-orbital blood draw for time zero was performed followed by opening a small incision to the lower abdomen of the pregnant mouse to expose a single conceptus for fetal tissue harvesting. Immediately after the first blood draw and fetus collection, a tail vein catheter was placed and bolus (4 µL/gBW) was administered followed by continuous infusion (0.085ul/gBW/min). A single conceptus, accessed via the initial small incision, was harvested at multiple time points during infusion of the nutrient tracer (13C glucose). Retro-orbital blood draws (<20 ul) were taken throughout the infusion to monitor tracer enrichment in maternal blood. Timepoints for harvesting after time zero were: 30 minutes, 1 hour, 2 hours, 3 hours, and 4 hours. |