Summary of project PR000146

Project ID: PR000146
Project DOI:doi: 10.21228/M83G6X
Project Title:Effect of Insulin Sensitizer Therapy on Amino Acids and Their Metabolites
Project Summary:Aims
Prior studies have reported that elevated concentrations of several plasma amino acids (AA), particularly branched chain (BCAA) and aromatic AA predict the onset of type 2 diabetes. We sought to test the hypothesis that circulating BCAA, aromatic AA and related AA metabolites decline in response to the use of insulin sensitizing agents in overweight/obese adults with impaired fasting glucose or untreated diabetes.
Methods
We performed a secondary analysis of a randomized, double-blind, placebo, controlled study conducted in twenty five overweight/obese (BMI ~ 30 kg/m2) adults with impaired fasting glucose or untreated diabetes. Participants were randomized to three months of pioglitazone (45 mg per day) plus metformin (1000 mg twice per day, N = 12 participants) or placebo (N = 13). We measured insulin sensitivity by the euglycemic-hyperinsulinemic clamp and fasting concentrations of AA and AA metabolites using ultra-pressure liquid chromatography tandem mass spectrometry before and after the three-month intervention.
Results
Insulin sensitizer therapy that significantly enhanced insulin sensitivity reduced 9 out of 33 AA and AA metabolites measured compared to placebo treatment. Moreover, insulin sensitizer therapy significantly reduced three functionally clustered AA and metabolite pairs: i) phenylalanine/tyrosine, ii) citrulline/arginine, and iii) lysine/?-aminoadipic acid.
Conclusions
Reductions in plasma concentrations of several AA and AA metabolites in response to three months of insulin sensitizer therapy support the concept that reduced insulin sensitivity alters AA and AA metabolites.
Institute:Mayo Clinic
Department:Endocrinology
Laboratory:Sree Nair
Last Name:Nair
First Name:Sree
Email:Dasari.Surendra@mayo.edu

Summary of all studies in project PR000146

Study IDStudy TitleSpeciesInstituteAnalysisRelease
Date
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ST000168 Effect of Insulin Sensitizer Therapy on Amino Acids and Their Metabolites Homo sapiens Mayo Clinic MS 2015-06-28 1 200 Uploaded data (928K)
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