Summary of project PR000705
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000705. The data can be accessed directly via it's Project DOI: 10.21228/M8JH5X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000705 |
| Project DOI: | doi: 10.21228/M8JH5X |
| Project Title: | Lipidomics for wildlife disease etiology and biomarker discovery: a case study of pansteatitis outbreak in South Africa |
| Project Type: | Lipidomics |
| Project Summary: | The lipidome of adipose tissue (n = 31) and plasma (n = 51) from Tilapia collected at Loskop Dam, South Africa, were characterized using state of the art liquid chromatography coupled to high-resolution tandem mass spectrometry. Lipid profiles reflected pansteatitis severity and were significantly different between diseased and healthy individuals. Over 13 classes of lipids associated with inflammation, cell death, and/or oxidative damage were upregulated in pansteatitis-affected adipose tissue, including ether-lipids, short-chained triglyceride oxidation products, sphingolipids, and acylcarnitines. Ceramides showed a 1000-fold increase in the most affected adipose tissues, illustrating its potential as sensitive and novel indicators of disease severity. In plasma, triglycerides were found to be downregulated in pansteatitis-affected tilapia. As comprehensive coverage of the lipidome aids in the elucidation of possible disease mechanisms, application of lipidomics could be applied to the understanding of other environmentally-derived inflammatory conditions, such as those caused by obesogens. |
| Institute: | South East Center for Integrated Metabolomics |
| Department: | Department of Pathology, Immunology and Laboratory Medicine |
| Laboratory: | SECIM |
| Last Name: | Koelmel |
| First Name: | Jeremy |
| Address: | Department of Pathology, Immunology and Laboratory Medicine, University of Florida, 1395 Center Dr, Room M641c, Gainesville, FL, 32610, USA |
| Email: | jeremykoelmel@gmail.com |
| Phone: | 7187300454 |
| Funding Source: | NIH Grant #U24 DK097209 |
| Publications: | submitted to Metabolomics |
| Contributors: | Jeremy P. Koelmel, Candice Z. Ulmer, Susan Fogelson, Christina M. Jones, Hannes Botha, Jacqueline T. Bangma, Theresa C. Guillette, Wilmien J. Luus-Powell, Joseph R. Sara, Willem J. Smit, Korin Albert, Harmony A. Miller, Matthew P. Guillette, Berkley C. Olsen, Jason A. Cochran, Timothy J. Garrett, Richard A. Yost, John A. Bowden |
Summary of all studies in project PR000705
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001052 | Lipidomics for wildlife disease etiology and biomarker discovery: a case study of pansteatitis outbreak in South Africa (part-I) | Oreochromis mossambicus | South East Center for Integrated Metabolomics | MS | 2018-09-27 | 1 | 53 | Uploaded data (30.2G)* |
| ST001059 | Lipidomics for wildlife disease etiology and biomarker discovery: a case study of pansteatitis outbreak in South Africa (part-II) | Oreochromis mossambicus | South East Center for Integrated Metabolomics | MS | 2018-09-27 | 1 | 31 | Uploaded data (19.2G)* |
