Summary of Study ST000606

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000443. The data can be accessed directly via it's Project DOI: 10.21228/M8SP5T This work is supported by NIH grant, U2C- DK119886.

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Study IDST000606
Study TitleBioprospection of the aromatic potential of species from the Atlantic Rainforest in São Paulo: occurrence, taxonomy and chemical, genetic and physiological characterization of plant populations
Study TypeChemcial profile of the volatiles from plants of the Atlantic Rainforest in São Paulo
Study SummaryNative plants were sampled from nine Atlantic rainforest locations in Brazil for botanical identification, herbarium mounts and chemical analyses. Plants were marked and the coordinate reference determined by Global Positioning System (GPS). Botanical names are as presented in the list of species of Brazilian flora (Lista de Espécies da Flora do Brasil, Jardim Botânico do Rio de Janeiro, 2015). Voucher specimens were deposited at the Herbarium of Instituto Agronômico de Campinas (IAC) (http://herbario.iac.sp.gov.br/), under the given accession numbers (Table 1). Marked plants were resampled in two subsequent years and those failing to be located or in poor condition were excluded from analyses. Environmental data were obtained from meteorological stations at the locations. Monthly values were used to calculate the average seasonal data.
Institute
Instituto Agronômico, IAC, São Paulo, Brazil
DepartmentCentro de Pesquisa e Desenvolvimento de Recursos Genéticos Vegetais
LaboratoryLaboratório de Produtos Naturais
Last NameOrtiz Mayo Marques
First NameMarcia
AddressAvenida Barão de Itapura, 1481, Botafogo, Campinas - SP, 13020-902, Brazil
Emailmortiz@iac.sp.gov.br
Phone+55(19) 3202-1770
Submit Date2017-05-14
Num Groups4
Total Subjects63
Raw Data File Type(s)d01
Analysis Type DetailGC-MS
Release Date2023-01-25
Release Version1
Marcia Ortiz Mayo Marques Marcia Ortiz Mayo Marques
https://dx.doi.org/10.21228/M8SP5T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000443
Project DOI:doi: 10.21228/M8SP5T
Project Title:Bioprospection of the aromatic potential of species from the Atlantic Rainforest in São Paulo: occurrence, taxonomy and chemical, genetic and physiological characterization of plant populations
Project Type:Atlantic Rainforest Chemical Characterization
Project Summary:Neotropical Atlantic Rainforest is a plant diversity rich biome and encompasses distinct vegetation formations depending on the environment. Essential oils (EOs) are part of the plants volatilome and consist of complex blends of hydrophobic metabolites. EOs are valuable for green chemistry applications. However, the interactions between the environment and plant processes controlling its chemical composition are poorly understood. The work aimed to provide a comprehensive characterization of the EOs from native species in distinct Atlantic Rainforest formations and access their antimicrobial potential. Aerial parts were harvested and dried for oil extraction by hydrodistillation, in two subsequent years. Chemical profiles were done by GC-MS and the metabolites identified by mass spectra and retention indices. Oil contents were determined by area normalization using GC–FID. Antimicrobial activity was investigated by in vitro growth inhibition. Computing and graphics were generated in R. The EO chemical profile was determined for plants from 50 species, in 15 botanical families, from nine Rainforest locations. Mono and sesquiterpenes were the most abundant chemical classes, followed by methyl ketones, phenylpropanoids and benzyl alcohols. Season and location were better discriminants for EO chemical profile than botanical family. Modules of co-regulated metabolites were identified and shown to correlate with environmental factors. Antimicrobial activity was widespread for the isolated EOs. Results provide a comprehensive metabolite profile of the EOs from the Atlantic Rainforest. Metabolite modules, correlated to environmental conditions, are conserved in complex plant populations. EOs are effective to inhibit pathogenic bacterial and fungal growth.
Institute:Instituto Agronômico de Campinas, IAC, São Paulo, Brazil
Department:Centro de Pesquisa e Desenvolvimento de Recursos Genéticos Vegetais
Laboratory:Laboratório de Produtos Naturais
Last Name:Ortiz Mayo Marques
First Name:Márcia
Address:Av Barão de Itapura, 1481, Botafogo, Campinas - SP, 13012-970, Brazil
Email:mortiz@iac.sp.ogv
Phone:+55(19) 3202-1770

Subject:

Subject ID:SU000629
Subject Type:Plants
Subject Species:Mixed
Age Or Age Range:Adult plants
Species Group:Plant

Factors:

Subject type: Plants; Subject species: Mixed (Factor headings shown in green)

mb_sample_id local_sample_id Plant Species
SA033438R1736/ IAC_47263Aiouea sp | Botanical Family:Lauraceae | Location:Pariquera-Açu | Season:Winter
SA033439C004/ IAC_4614Aloysia virgata (Ruiz & Pav.) Juss. | Botanical Family:Verbenaceae | Location:Jundiai | Season:Winter
SA033440R1746/ IAC_47273Aniba viridis Mez | Botanical Family:Lauraceae | Location:Pariquera-Açu | Season:Winter
SA033441Lp51907/ IAC_47071Aniba viridis Mez | Botanical Family:Lauraceae | Location:Ubatuba | Season:Autumn
SA033442Lp06101603/ IAC_47955Annona dioica A.St.-Hil. | Botanical Family:Annonaceae | Location:Votuporanga | Season:Spring
SA033443R1755/ IAC_47282Baccharis dracunculifolia DC. | Botanical Family:Asteraceae | Location:Pariquera-Açu | Season:Winter
SA033444R1773/ IAC_47522Baccharis dracunculifolia DC. | Botanical Family:Asteraceae | Location:Ribeirão Preto | Season:Spring
SA033445R1745/IAC_47272Calyptranthes lanceolata O.Berg | Botanical Family:Myrtaceae | Location:Pariquera-Açu | Season:Winter
SA033446Lp52009/ IAC_47080Calyptranthes lucida Mart. ex DC. | Botanical Family:Myrtaceae | Location:Ubatuba | Season:Autumn
SA033447Lp6101822/ IAC_47988Campomanesia guavirota (DC.) Kiaersk. | Botanical Family:Myrtaceae | Location:Adamantina | Season:Spring
SA033448R1643/ IAC_46842Casearia sylvestris Sw. | Botanical Family:Salicaceae | Location:Ubatuba | Season:Summer
SA033449Lp52912/IAC_29030Croton celtidifolius Baill. | Botanical Family:Euphorbiaceae | Location:Monte Alegre do Sul | Season:Autumn
SA033450R4167/ IAC_46976Croton floribundus Spreng. | Botanical Family:Euphorbiaceae | Location:Campinas | Season:Autumn
SA033451R1768/ IAC_47517Croton urucurana Baill. | Botanical Family:Euphorbiaceae | Location:Mococa | Season:Summer
SA033452R4165/ IAC_46974Croton warmingii Müll. Arg. | Botanical Family:Euphorbiaceae | Location:Campinas | Season:Autumn
SA033453L4160/ IAC_46969Cupania vernalis Cambess. | Botanical Family:Sapindaceae | Location:Campinas | Season:Autumn
SA033454C005/ IAC_47097Cyrtocymura scorpioides (Lam.) H. Rob. | Botanical Family:Asteraceae | Location:Jundiai | Season:Winter
SA033455Lp6101818/ IAC_47975Dendropanax cuneatus (DC.) Decne. & Planch. | Botanical Family:Araliaceae | Location:Adamantina | Season:Spring
SA033456C010/ IAC_47099Dendropanax cuneatus (DC.) Decne. & Planch. | Botanical Family:Araliaceae | Location:Jundiai | Season:Winter
SA033457R1770/ IAC_47519Dendropanax cuneatus (DC.) Decne. & Planch. | Botanical Family:Araliaceae | Location:Mococa | Season:Spring
SA033458R1622/ IAC_46801Endlicheria paniculata (Spreng.) J.F.Macbr. | Botanical Family:Lauraceae | Location:Campinas | Season:Summer
SA033459Lp06091205/ IAC_44591Esenbeckia febrifuga (A.St.-Hil.) A.Juss. ex Mart | Botanical Family:Rutaceae | Location:Campinas | Season:Spring
SA033460Lp06101816/ IAC_47973Eugenia moraviana O.Berg. | Botanical Family:Myrtaceae | Location:Adamantina | Season:Spring
SA033461Lp6101714/ IAC_47971Eugenia myrcianthes Nied. | Botanical Family:Myrtaceae | Location:Votuporanga | Season:Spring
SA033462R1626/ IAC_46825Eugenia neoverrucosa Sobral | Botanical Family:Myrtaceae | Location:Campinas | Season:Summer
SA033463Lp51905/ IAC_47069Eugenia prasina O.Berg | Botanical Family:Myrtaceae | Location:Ubatuba | Season:Autumn
SA033464C009/ IAC_34660Eugenia pyriformis Cambess. | Botanical Family:Myrtaceae | Location:Jundiai | Season:Winter
SA033465R1598/ IAC_46831Guatteria australis A.St.-Hil. | Botanical Family:Annonaceae | Location:Ubatuba | Season:Summer
SA033466Lp6101823/ IAC_47989Helietta apiculata Benth. | Botanical Family:Rutaceae | Location:Adamantina | Season:Spring
SA033467Lp52006/IAC_47077Marlierea exocoriata Mart. | Botanical Family:Myrtaceae | Location:Ubatuba | Season:Autumn
SA033468Lp06091204/ IAC_46826Metrodorea nigra A.St.-Hil. | Botanical Family:Rutaceae | Location:Campinas | Season:Spring
SA033469L52008/ IAC_47079Monstera cf. adansonii Schott | Botanical Family:Araceae | Location:Ubatuba | Season:Autumn
SA033470Lp051901/IAC_47045Myrcia spectabilis DC. | Botanical Family:Myrtaceae | Location:Ubatuba | Season:Autumn
SA033471C007/ IAC_37365Myrcia splendens (Sw.) DC. | Botanical Family:Myrtaceae | Location:Jundiai | Season:Winter
SA033472Lp06101817/IAC_47974Myrcia tomentosa (Aubl.) DC. | Botanical Family:Myrtaceae | Location:Adamantina | Season:Spring
SA033473Lp6101820/ IAC_47986Nectandra megapotamica (Spreng.) Mez | Botanical Family:Lauraceae | Location:Adamantina | Season:Spring
SA033474R1761/ IAC_47510Nectandra megapotamica (Spreng.) Mez | Botanical Family:Lauraceae | Location:Mococa | Season:Spring
SA033475R1774/ IAC_47523Nectandra megapotamica (Spreng.) Mez | Botanical Family:Lauraceae | Location:Ribeirão Preto | Season:Spring
SA033476Lp052902/IAC_47084Nectranda megapotamica (Spreng.) Mez | Botanical Family:Lauraceae | Location:Monte Alegre do Sul | Season:Autumn
SA033477R1747/ IAC_47274Ocotea odorifera (Vell.) Rohwer | Botanical Family:Lauraceae | Location:Pariquera-Açu | Season:Winter
SA033478Lp52911/IAC_47090Piper aduncum L. | Botanical Family:Piperaceaea | Location:Monte Alegre do Sul | Season:Winter
SA033479Lp6101606/IAC_47958Piper aduncum L. | Botanical Family:Piperaceaea | Location:Votuporanga | Season:Spring
SA033480Lp6101608/IAC_47960Piper aduncum L. | Botanical Family:Piperaceaea | Location:Votuporanga | Season:Spring
SA033481Lp6101821/IAC_47987Piper amalago L. | Botanical Family:Piperaceaea | Location:Adamantina | Season:Spring
SA033482Lp06091202/IAC_32056Piper amalago L. | Botanical Family:Piperaceaea | Location:Campinas | Season:Spring
SA033483Lp06091206/IAC_46823Piper amalago L. | Botanical Family:Piperaceaea | Location:Campinas | Season:Summer
SA033484R1763/IAC_47512Piper amalago L. | Botanical Family:Piperaceaea | Location:Mococa | Season:Winter
SA033485R1740/IAC_7267Piper amplum Kunth. | Botanical Family:Piperaceaea | Location:Pariquera-Açu | Season:Winter
SA033486R1741/IAC_7268Piper cernuum Vell. | Botanical Family:Piperaceaea | Location:Pariquera-Açu | Season:Winter
SA033487L51904/ IAC_7068Piper cernuum Vell. | Botanical Family:Piperaceaea | Location:Ubatuba | Season:Autumn
SA033488R1764/IAC_7513Piper crassinervium Kunth. | Botanical Family:Piperaceaea | Location:Mococa | Season:Winter
SA033489R1738/IAC_7265Piper gaudichaudianum Kunth. | Botanical Family:Piperaceaea | Location:Pariquera-Açu | Season:Winter
SA033490Lp052903/ IAC_7085Piper leptorum Kunth. | Botanical Family:Piperaceaea | Location:Monte Alegre do Sul | Season:Autumn
SA033491L52007/ IAC_47078Piper rivinoides Kunth. | Botanical Family:Piperaceaea | Location:Ubatuba | Season:Winter
SA033492R1633/ IAC_46832Piper solmsianum C.DC. | Botanical Family:Piperaceaea | Location:Ubatuba | Season:Summer
SA033493R4169/IAC_46978Piper umbellatum (L.) | Botanical Family:Piperaceaea | Location:Campinas | Season:Autumn
SA033494L52004/IAC_47075Piper xylosteoides (Kunth.) Steud. | Botanical Family:Piperaceaea | Location:Ubatuba | Season:Autumn
SA033495R1772/IAC_47521Schinus terebinthifolius Raddi | Botanical Family:Anacardiaceae | Location:Ribeirão Preto | Season:Spring
SA033496Lp6101709/IAC_47961Trichilia elegans A.Juss. | Botanical Family:Meliaceae | Location:Votuporanga | Season:Spring
SA033497Lp52003/ IAC_49465Virola bicuhyba (Schott ex Spreng.) Warb. | Botanical Family:Myristicaceae | Location:Ubatuba | Season:Autumn
SA033498Lp6101712/ IAC_47969Xylopia aromatica (Lam.) Mart. | Botanical Family:Annonaceae | Location:Votuporanga | Season:Spring
SA033499R1739/ IAC_47266Xylopia brasiliensis Spreng. | Botanical Family:Annonaceae | Location:Pariquera-Açu | Season:Winter
SA033500Lp6101710/ IAC_47962Zanthoxylum petiolare A.St.-Hil. & Tul. | Botanical Family:Rutaceae | Location:Votuporanga | Season:Spring
Showing results 1 to 63 of 63

Collection:

Collection ID:CO000623
Collection Summary:Leaves were detached from woody stalks and air dried at room temperature, in the absence of direct light. EOs were extracted from 54 to 1870 g of dry material depending on availability, using a Clevenger-type apparatus until total recovery. Oils were transferred to hermetically closed vials, and stored at -20oC. Yield was is presented as oil weight (g) per plant material weight (g). Extractions were performed independently for each collection, and averaged for two collection years.
Collection Protocol Comments:Plants failing to be located or in poor condition were excluded from analyses
Collection Method:Aerial parts harvesting
Collection Location:Rainforest locations in the state of São Paulo, Brazil (Adamantina, Campinas, Jundiai, Mococa, Monte Alegre do Sul, Pariquera-Açu, Ribeirão Preto, Ubatuba, Votuporanga)
Collection Frequency:Once a season, every two years
Storage Conditions:Detached leaves were air dried at room temperature, in the absence of light. Isolated essential oils were stored in hermetically closed vials at -20C.
Storage Vials:Hermetically closed glass vials, kept at -20C

Treatment:

Treatment ID:TR000643
Treatment Summary:Native plants were sampled from nine Atlantic rainforest locations in Brazil (Adamantina, Campinas, Jundiaí, Mococa, Monte Alegre do Sul, Pariquera-Açú, Ribeirão Preto, Ubatuba, Votuporanga) for botanical identification, herbarium mounts and chemical analyses. Plants were marked and the coordinate reference determined by Global Positioning System (GPS). Botanical names are as presented in the list of species of Brazilian flora (Lista de Espécies da Flora do Brasil, Jardim Botânico do Rio de Janeiro, 2015). Voucher specimens were deposited at the Herbarium of Instituto Agronômico de Campinas (IAC) (http://herbario.iac.sp.gov.br/), under the given accession numbers (Study Design tab). Marked plants were resampled in two subsequent years and those failing to be located or in poor condition were excluded from analyses.
Plant Growth Support:Native soil
Plant Growth Location:Nine Rainforest Locations in the state of São Paulo (Brazil) : Adamantina, Campinas, Jundiai, Mococa, Monte Alegre do Sul, Pariquera-Açu, Ribeirão Preto, Ubatuba, Votuporanga
Plant Plot Design:Native plants were identified, marked and coordinate reference locations were determined by Global Positioning System (GPS). Marked plants were resampled, throughout the year, in two subsequent years. The individuals failing to be located or in poor condition were excluded from the analyses.
Plant Light Period:Natural daylight conditions
Plant Humidity:Natural humidity conditions
Plant Temp:Natural temperature conditions
Plant Watering Regime:Natural rainfall watering
Plant Nutritional Regime:Natural soil nutrition
Plant Growth Stage:Mature
Plant Metab Quench Method:Air drying
Plant Harvest Method:Cuttings from aerial parts

Sample Preparation:

Sampleprep ID:SP000636
Sampleprep Summary:EOs were extracted from 54 to 1870 g of dry material depending on availability, using a Clevenger-type apparatus until total recovery.
Processing Method:Detached leaves were air dried at room temperature in the absence of light
Extraction Method:Hydrodistillation with Clevenger-type apparatus
Organ:Aerial parts (leaves and petioles)

Combined analysis:

Analysis ID AN000927
Analysis type MS
Chromatography type GC
Chromatography system Shimadzu GC-2010
Column fused silica capillary column OV - 5, dimensions 30m x 0.25mm x 0.25μm (Ohio Valley Specialty Chemical, Inc., USA)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Shimadzu QP-5000
Ion Mode POSITIVE
Units % peak area

Chromatography:

Chromatography ID:CH000662
Chromatography Summary:Targeted GC-MS and GC-FID. GC-MS using a Shimadzu QP-5000, equipped with fused silica capillary column OV - 5 (30m x 0.25mm x 0.25μm, Ohio Valley Specialty Chemical, Inc., USA), operating with electron impact (70 eV), Helium (1.0 mL/min.), injector at 220 °C, detector at 230 °C, split ratio 1/30 and the following temperature program: 60 0C- 240 0C, 3 0C/min. GC–FID at a Shimadzu GC-2010 equipped with fused silica capillary column OV - 5 (30m x 0.25mm x 0.25μm, Ohio Valley Specialty Chemical, Inc., USA), operating with electron impact (70 eV), Helium (1.0 mL/min.), injector at 220 °C, detector at 230 °C, split ratio 1/30 and the following temperature program: 60°C- 240 0C, 3 0C/min.
Instrument Name:Shimadzu GC-2010
Column Name:fused silica capillary column OV - 5, dimensions 30m x 0.25mm x 0.25μm (Ohio Valley Specialty Chemical, Inc., USA)
Flow Rate:1.0 mL/min (Helium)
Injection Temperature:220 °C
Retention Index:obtained from the injection of a mixture of n-alcanes (C9H20–C25H52, Sigma Aldrich, 99%)
Oven Temperature:60 °C- 240 °C, 3 0C/min
Chromatography Type:GC

MS:

MS ID:MS000824
Analysis ID:AN000927
Instrument Name:Shimadzu QP-5000
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:EI/qradupole/Electron multiplier
Ion Mode:POSITIVE
Fragmentation Method:electron impact
Helium Flow:1.0 mL/min
Ionization:EI
Ionization Energy:70 eV
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