Summary of Study ST000005

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000004. The data can be accessed directly via it's Project DOI: 10.21228/M8MW26 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000005
Study TitleTimecourse on RAW 264.7 cells treated with Kdo2-Lipid A and compactin
Study TypeTimecourse experiment
Study SummaryLipidomics studies on macrophages - RAW 264.7 cells treated with Kdo2-Lipid A and compactin. Experiments were conducted with RAW264.7 cells fed 10% fetal calf serum. 8-timepoint study: Measurements were taken at 0, 0.5,1,2,4,8, 12, and 24hrs for: (i) compactin, (ii) Kdo2-Lipid A, (iii) compactin + Kdo2-Lipid A. and (iv) control
Institute
LIPID MAPS
DepartmentMultiple
LaboratoryMultiple
Last NameFahy
First NameEoin
Emailefahy@ucsd.edu
Submit Date2013-02-20
PublicationsDennis, E.A. et al A mouse macrophage lipidome. J. Biol. Chem., 285, 39976-39985 (2010).
Raw Data AvailableNo
Analysis Type DetailGC-MS/LC-MS
Release Date2013-03-22
Release Version1
Eoin Fahy Eoin Fahy
https://dx.doi.org/10.21228/M8MW26
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000004
Project DOI:doi: 10.21228/M8MW26
Project Title:LIPID MAPS Lipidomics studies
Project Type:MS quantitative analysis
Project Summary:Multi-center quantitative lipidomics studies on samples from human and murine sources (LIPIDMAPS)
Institute:LIPID MAPS
Department:Multiple
Laboratory:Multiple
Last Name:Fahy
First Name:Eoin
Address:UCSD
Email:efahy@ucsd.edu
Funding Source:NIGMS

Subject:

Subject ID:SU000006
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:RAW 264.7
Species Group:Mammals

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Hours Compactin KLA
SA054136KS0130.5 50uM 100ng/ml
SA054137KS0140.5 50uM 100ng/ml
SA054138KS0150.5 50uM 100ng/ml
SA054139KS0160.5 50uM 100ng/ml
SA054128KS0050.5 50uM None
SA054129KS0060.5 50uM None
SA054130KS0070.5 50uM None
SA054131KS0080.5 50uM None
SA054132KS0090.5 None 100ng/ml
SA054133KS0100.5 None 100ng/ml
SA054134KS0110.5 None 100ng/ml
SA054135KS0120.5 None 100ng/ml
SA054124KS0010.5 None None
SA054125KS0020.5 None None
SA054126KS0030.5 None None
SA054127KS0040.5 None None
SA054140KS0170 None None
SA054141KS0180 None None
SA054142KS0190 None None
SA054143KS0200 None None
SA054172KS04912 50uM 100ng/ml
SA054173KS05012 50uM 100ng/ml
SA054174KS05112 50uM 100ng/ml
SA054175KS05212 50uM 100ng/ml
SA054164KS04112 50uM None
SA054165KS04212 50uM None
SA054166KS04312 50uM None
SA054167KS04412 50uM None
SA054168KS04512 None 100ng/ml
SA054169KS04612 None 100ng/ml
SA054170KS04712 None 100ng/ml
SA054171KS04812 None 100ng/ml
SA054160KS03712 None None
SA054161KS03812 None None
SA054162KS03912 None None
SA054163KS04012 None None
SA054156KS0331 50uM 100ng/ml
SA054157KS0341 50uM 100ng/ml
SA054158KS0351 50uM 100ng/ml
SA054159KS0361 50uM 100ng/ml
SA054148KS0251 50uM None
SA054149KS0261 50uM None
SA054150KS0271 50uM None
SA054151KS0281 50uM None
SA054152KS0291 None 100ng/ml
SA054153KS0301 None 100ng/ml
SA054154KS0311 None 100ng/ml
SA054155KS0321 None 100ng/ml
SA054144KS0211 None None
SA054145KS0221 None None
SA054146KS0231 None None
SA054147KS0241 None None
SA054204KS08124 50uM 100ng/ml
SA054205KS08224 50uM 100ng/ml
SA054206KS08324 50uM 100ng/ml
SA054207KS08424 50uM 100ng/ml
SA054196KS07324 50uM None
SA054197KS07424 50uM None
SA054198KS07524 50uM None
SA054199KS07624 50uM None
SA054200KS07724 None 100ng/ml
SA054201KS07824 None 100ng/ml
SA054202KS07924 None 100ng/ml
SA054203KS08024 None 100ng/ml
SA054192KS06924 None None
SA054193KS07024 None None
SA054194KS07124 None None
SA054195KS07224 None None
SA054188KS0652 50uM 100ng/ml
SA054189KS0662 50uM 100ng/ml
SA054190KS0672 50uM 100ng/ml
SA054191KS0682 50uM 100ng/ml
SA054180KS0572 50uM None
SA054181KS0582 50uM None
SA054182KS0592 50uM None
SA054183KS0602 50uM None
SA054184KS0612 None 100ng/ml
SA054185KS0622 None 100ng/ml
SA054186KS0632 None 100ng/ml
SA054187KS0642 None 100ng/ml
SA054176KS0532 None None
SA054177KS0542 None None
SA054178KS0552 None None
SA054179KS0562 None None
SA054220KS0974 50uM 100ng/ml
SA054221KS0984 50uM 100ng/ml
SA054222KS0994 50uM 100ng/ml
SA054223KS1004 50uM 100ng/ml
SA054212KS0894 50uM None
SA054213KS0904 50uM None
SA054214KS0914 50uM None
SA054215KS0924 50uM None
SA054216KS0934 None 100ng/ml
SA054217KS0944 None 100ng/ml
SA054218KS0954 None 100ng/ml
SA054219KS0964 None 100ng/ml
SA054208KS0854 None None
SA054209KS0864 None None
SA054210KS0874 None None
SA054211KS0884 None None
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Collection:

Collection ID:CO000005
Collection Summary:-
Sample Type:Macrophages

Treatment:

Treatment ID:TR000007

Sample Preparation:

Sampleprep ID:SP000010
Sampleprep Summary:-

Combined analysis:

Analysis ID AN000012
Chromatography ID CH000005
MS ID MS000012
Analysis type MS
Chromatography type
Chromatography system Multiple
Column Multiple
MS Type Multiple
MS instrument type Multiple
MS instrument name Multiple
Ion Mode UNSPECIFIED
Units pmol/ug DNA

Chromatography:

Chromatography ID:CH000005
Instrument Name:Multiple
Column Name:Multiple

MS:

MS ID:MS000012
Analysis ID:AN000012
Instrument Name:Multiple
Instrument Type:Multiple
MS Type:Multiple
Ion Mode:UNSPECIFIED
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