Summary of Study ST000176
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000148. The data can be accessed directly via it's Project DOI: 10.21228/M8V011 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000176 |
| Study Title | cell and liver metabolomics |
| Study Summary | (Pending) Liver samples were harvested from wt and ko mice. Quadruplicates and triplicates are sent for cells and liver, respectively. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@umich.edu | |
| Submit Date | 2014-05-12 |
| Num Groups | 4 |
| Total Subjects | 14 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Uploaded File Size | 3.1 G |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2015-06-28 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000148 |
| Project DOI: | doi: 10.21228/M8V011 |
| Project Title: | trcp |
| Project Summary: | metabolic phenotypes of a clock mutant mouse |
| Institute: | Florida State University |
| Department: | Biomedical Sciences |
| Laboratory: | Lee Lab |
| Last Name: | Lee |
| First Name: | Choogon |
| Address: | Tallahassee, FL |
| Email: | choogon.lee@med.fsu.edu |
| Phone: | 850-645-1478 |
Subject:
| Subject ID: | SU000195 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | genotype | Type |
|---|---|---|---|
| SA009475 | S00014601 | background | liver |
| SA009476 | S00014600 | background | liver |
| SA009477 | S00014602 | background | liver |
| SA009471 | S00014608 | background | MFE Cells |
| SA009472 | S00014609 | background | MFE Cells |
| SA009473 | S00014607 | background | MFE Cells |
| SA009474 | S00014606 | background | MFE Cells |
| SA009482 | S00014604 | knock-out | liver |
| SA009483 | S00014605 | knock-out | liver |
| SA009484 | S00014603 | knock-out | liver |
| SA009478 | S00014612 | knock-out | MFE Cells |
| SA009479 | S00014613 | knock-out | MFE Cells |
| SA009480 | S00014611 | knock-out | MFE Cells |
| SA009481 | S00014610 | knock-out | MFE Cells |
| Showing results 1 to 14 of 14 |
Collection:
| Collection ID: | CO000182 |
| Collection Summary: | - |
| Sample Type: | Cells |
Treatment:
| Treatment ID: | TR000202 |
Sample Preparation:
| Sampleprep ID: | SP000196 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx |
Combined analysis:
| Analysis ID | AN000270 | AN000271 |
|---|---|---|
| Chromatography ID | CH000193 | CH001303 |
| MS ID | MS000219 | MS000220 |
| Analysis type | MS | MS |
| Chromatography type | HILIC | GC |
| Chromatography system | Agilent 1260 | Agilent 7890A |
| Column | Phenomenex Luna NH2 (150 x 1mm,3um) | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| MS Type | ESI | EI |
| MS instrument type | QTOF | Single quadrupole |
| MS instrument name | Agilent 6530 QTOF | Agilent 5975C |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | pmol/mg protein | SUC |
Chromatography:
| Chromatography ID: | CH000193 |
| Methods ID: | AQM020 |
| Methods Filename: | QTOF-002-HILIC-35min-1mm-No_Insert.m.zip |
| Instrument Name: | Agilent 1260 |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001303 |
| Methods ID: | AQM011 |
| Methods Filename: | ALPHA_KETO_ACIDS-FULL.M.zip |
| Instrument Name: | Agilent 7890A |
| Column Name: | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000219 |
| Analysis ID: | AN000270 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Acquisition Parameters File: | QTOF-002-HILIC-35min-1mm-No_Insert.m.zip |
| Processing Parameters File: | EX00296-MassHunterQuant-GlyTCA-DataAnalysis-Method.m.zip |
| MS ID: | MS000220 |
| Analysis ID: | AN000271 |
| Instrument Name: | Agilent 5975C |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
| Acquisition Parameters File: | ALPHA_KETO_ACIDS-FULL.M.zip |
