Summary of Study ST000300
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000241. The data can be accessed directly via it's Project DOI: 10.21228/M8H88B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000300 |
Study Title | Chicken/C. jejuni Bile Acid Study |
Study Summary | Cecal samples were isolated directly from the ceca of dissected chickens that were either experimentally infected with C. jejuni DRH212 or mock-infected with PBS. Cecal samples were re-suspended in Life Technologies 1X PBS based on weight of sample (1 ml/100 mg = 10-1 dilution) and stored at -80C. |
Institute | University of Michigan |
Department | Biomedical Research Core Facilities |
Laboratory | Metabolomics core |
Last Name | Kachman |
First Name | Maureen |
Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
mkachman@med.umich.edu | |
Phone | (734) 232-8175 |
Submit Date | 2015-03-17 |
Num Groups | 2 |
Total Subjects | 4 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2016-06-18 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000241 |
Project DOI: | doi: 10.21228/M8H88B |
Project Title: | Identifiying Colonization Factors of Campylobacter jejuni |
Project Summary: | Using next-generation approaches to identify both bacterial and host factors that influence colonization of the chicken gastrointestinal tract by C. jejuni |
Institute: | University of Michigan |
Department: | Microbiology and Immunology |
Laboratory: | DiRita Lab |
Last Name: | DiRita |
First Name: | Victor |
Address: | Ann Arbor, MI |
Email: | vdirita@umich.edu |
Phone: | 734-936-3804 |
Subject:
Subject ID: | SU000320 |
Subject Type: | Animal |
Subject Species: | Gallus gallus |
Taxonomy ID: | 9031 |
Species Group: | Bird |
Factors:
Subject type: Animal; Subject species: Gallus gallus (Factor headings shown in green)
mb_sample_id | local_sample_id | C. jejuni Infection |
---|---|---|
SA013604 | S00017777 | no |
SA013605 | S00017776 | no |
SA013606 | S00017775 | yes |
SA013607 | S00017774 | yes |
Showing results 1 to 4 of 4 |
Collection:
Collection ID: | CO000314 |
Collection Summary: | - |
Sample Type: | Cecum |
Treatment:
Treatment ID: | TR000334 |
Treatment Summary: | - |
Sample Preparation:
Sampleprep ID: | SP000328 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | Bile Acids-20150305.docx |
Combined analysis:
Analysis ID | AN000478 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 1260 |
Column | Waters Acquity BEH C18 (50 x 2mm,1.7um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 6410 QQQ |
Ion Mode | NEGATIVE |
Units | uM |
Chromatography:
Chromatography ID: | CH000341 |
Methods Filename: | 2014-07-30-Bile_acid-full-BEH-C18-I.m.zip |
Instrument Name: | Agilent 1260 |
Column Name: | Waters Acquity BEH C18 (50 x 2mm,1.7um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000418 |
Analysis ID: | AN000478 |
Instrument Name: | Agilent 6410 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | Bile_Acids-20150305.docx |