Summary of Study ST000509

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000382. The data can be accessed directly via it's Project DOI: 10.21228/M8P597 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000509
Study TitleMetabolic changes to maternal rat liver tissue during and post-pregnancy (part I)
Study SummaryAssessing metabolic changes to maternal rat liver tissue during and post-pregnancy. Liver tissue was harvested post-mortem from rats or mice without pregnancy (Nulliparous - NP), time course during pregnancy in days (P2-4, P11-13m P18-20), lactation day 10 (LD10), in during involution of the liver (I2, I4, I6, I8, I10) and 4-weeks regression after preganancy (R4).
Institute
University of Colorado Denver
Last NameNemkov
First NameTravis
Address12801 E 17th Avenue RC-1 South, Mail stop 8101
Emailtravis.nemkov@ucdenver.edu
Phone303-724-5798
Submit Date2016-12-04
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2017-01-30
Release Version1
Travis Nemkov Travis Nemkov
https://dx.doi.org/10.21228/M8P597
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000382
Project DOI:doi: 10.21228/M8P597
Project Title:Metabolic changes to maternal rat liver tissue during and post-pregnancy.
Project Type:global MS analysis
Project Summary:Assessing metabolic changes to maternal rat liver tissue during and post-pregnancy. Liver tissue was harvested post-mortem from rats or mice without pregnancy (Nulliparous - NP), time course during pregnancy in days (P2-4, P11-13m P18-20), lactation day 10 (LD10), in during involution of the liver (I2, I4, I6, I8, I10) and 4-weeks regression after preganancy (R4).
Institute:University of Colorado Denver
Last Name:Nemkov
First Name:Travis
Address:12801 E 17th Avenue, RC-1 South, Rm 9403G, Aurora, CO, 80045, USA
Email:travis.nemkov@ucdenver.edu
Phone:303-724-5798

Subject:

Subject ID:SU000531
Subject Type:Animal
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Species Group:Mammals

Factors:

Subject type: Animal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id time_point
SA026500I10_60I10
SA026501I10_70I10
SA026502I10_64I10
SA026503I10_73I10
SA026504I2_90I2
SA026505I2_93I2
SA026506I2_104I2
SA026507I2_79I2
SA026508I2_92I2
SA026509I4_72I4
SA026510I4_101I4
SA026511I4_71I4
SA026512I4_96I4
SA026513I6_81I6
SA026514I6_94I6
SA026515I6_95I6
SA026516I6_77I6
SA026517I6_66I6
SA026518I8_75I8
SA026519I8_98I8
SA026520I8_97I8
SA026521I8_63I8
SA026522I8_67I8
SA026523I8_74I8
SA026524LD10_99LD10
SA026525LD10_9LD10
SA026526LD10_22LD10
SA026527LD10_31LD10
SA026528LD10_103LD10
SA026529NP_91NP
SA026530NP_58NP
SA026531NP_57NP
SA026532NP_102NP
SA026533NP_3NP
SA026534NP_2NP
SA026535P11-13_25P11-13
SA026536P11-13_17P11-13
SA026537P11-13_16P11-13
SA026538P11-13_18P11-13
SA026539P18-20_23P18-20
SA026540P18-20_76P18-20
SA026541P18-20_61P18-20
SA026542P18-20_69P18-20
SA026543P18-20_7P18-20
SA026544P18-20_15P18-20
SA026545P2-4_13P2-4
SA026546P2-4_12P2-4
SA026547P2-4_11P2-4
SA026548P2-4_14P2-4
SA026549P2-4_10P2-4
SA026550R4_83R4
SA026551R4_62R4
SA026552R4_30R4
SA026553R4_24R4
SA026554R4_26R4
SA026555R4_32R4
Showing results 1 to 56 of 56

Collection:

Collection ID:CO000525
Collection Summary:tissue was harvested, flash frozen in LiN2, milled with LiN2, and weighed on dry ice for metabolomics analysis
Sample Type:Liver

Treatment:

Treatment ID:TR000545
Treatment Summary:Tissues were harvested at the appropriate time point during or post-pregnancy

Sample Preparation:

Sampleprep ID:SP000538
Sampleprep Summary:Frozen and milled liver tissue was suspended to 10 mg/ml in an extraction solution containing methanol/acetonitrile/water at a ratio of 5:3:2 (v/v/v). Suspensions were vortexed at 4C for 30 minutes and centrifuged at 10,000 g for 10 minutes, 4C. Supernatants were isolated and analyzed by LC-MS.

Combined analysis:

Analysis ID AN000780 AN000781
Chromatography ID CH000559 CH000559
MS ID MS000687 MS000688
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish
Column Kinetex XB-C18 Kinetex XB-C18
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units arbitrary units arbitrary units

Chromatography:

Chromatography ID:CH000559
Instrument Name:Thermo Vanquish
Column Name:Kinetex XB-C18
Chromatography Type:Reversed phase

MS:

MS ID:MS000687
Analysis ID:AN000780
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS000688
Analysis ID:AN000781
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
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