Summary of Study ST000510

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000382. The data can be accessed directly via it's Project DOI: 10.21228/M8P597 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000510
Study TitleAssessing metabolic changes to maternal rat liver tissue during and post-pregnancy (part II)
Study SummaryAssessing metabolic changes to maternal rat liver tissue during and post-pregnancy. Liver tissue was harvested post-mortem from rats or mice without pregnancy (Nulliparous - NP), time course during pregnancy in days (P2-4, P11-13m P18-20), lactation day 10 (LD10), in during involution of the liver (I2, I4, I6, I8, I10) and 4-weeks regression after preganancy (R4).
Institute
University of Colorado Denver
Last NameNemkov
First NameTravis
Address12801 E 17th Avenue Mail Stop 8101
Emailtravis.nemkov@ucdenver.edu
Phone3037245798
Submit Date2016-12-05
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2017-01-30
Release Version1
Travis Nemkov Travis Nemkov
https://dx.doi.org/10.21228/M8P597
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000382
Project DOI:doi: 10.21228/M8P597
Project Title:Metabolic changes to maternal rat liver tissue during and post-pregnancy.
Project Type:global MS analysis
Project Summary:Assessing metabolic changes to maternal rat liver tissue during and post-pregnancy. Liver tissue was harvested post-mortem from rats or mice without pregnancy (Nulliparous - NP), time course during pregnancy in days (P2-4, P11-13m P18-20), lactation day 10 (LD10), in during involution of the liver (I2, I4, I6, I8, I10) and 4-weeks regression after preganancy (R4).
Institute:University of Colorado Denver
Last Name:Nemkov
First Name:Travis
Address:12801 E 17th Avenue, RC-1 South, Rm 9403G, Aurora, CO, 80045, USA
Email:travis.nemkov@ucdenver.edu
Phone:303-724-5798

Subject:

Subject ID:SU000532
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id time_point
SA026556I2_17I2
SA026557I2_224I2
SA026558I2_39I2
SA026559I2_34I2
SA026560I2_31I2
SA026561I2_229I2
SA026562I4_18I4
SA026563I4_215I4
SA026564I4_211I4
SA026565I4_202I4
SA026566I4_13I4
SA026567I4_15I4
SA026568I6_209I6
SA026569I6_218I6
SA026570I6_230I6
SA026571I6_232I6
SA026572I6_201I6
SA026573I6_216I6
SA026574I8_14I8
SA026575I8_99bI8
SA026576I8_98bI8
SA026577I8_101bI8
SA026578LD10_213LD10
SA026579LD10_236LD10
SA026580LD10_240LD10
SA026581LD10_237LD10
SA026582LD10_204LD10
SA026583LD10_200LD10
SA026584NP_226NP
SA026585NP_221NP
SA026586NP_227NP
SA026587NP_225NP
SA026588NP_220NP
SA026589NP_222NP
SA026590R4_243R4
SA026591R4_239R4
SA026592R4_214R4
SA026593R4_203R4
SA026594R4_232R4
Showing results 1 to 39 of 39

Collection:

Collection ID:CO000526
Collection Summary:tissue was harvested, flash frozen in LiN2, milled with LiN2, and weighed on dry ice for metabolomics analysis
Sample Type:Liver

Treatment:

Treatment ID:TR000546
Treatment Summary:Tissues were harvested at the appropriate time point during or post-pregnancy

Sample Preparation:

Sampleprep ID:SP000539
Sampleprep Summary:Frozen and milled liver tissue was suspended to 10 mg/ml in an extraction solution containing methanol/acetonitrile/water at a ratio of 5:3:2 (v/v/v). Suspensions were vortexed at 4C for 30 minutes and centrifuged at 10,000 g for 10 minutes, 4C. Supernatants were isolated and analyzed by LC-MS.

Combined analysis:

Analysis ID AN000782 AN000783
Chromatography ID CH000560 CH000560
MS ID MS000689 MS000690
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish
Column Kinetex C18-XB Kinetex C18-XB
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units arbitrary units arbitrary units

Chromatography:

Chromatography ID:CH000560
Instrument Name:Thermo Vanquish
Column Name:Kinetex C18-XB
Chromatography Type:Reversed phase

MS:

MS ID:MS000689
Analysis ID:AN000782
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS000690
Analysis ID:AN000783
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
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