Summary of Study ST000676
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000478. The data can be accessed directly via it's Project DOI: 10.21228/M88G7V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000676 |
| Study Title | Effect of high fat diet and streptozotocin treatment on neuropathy |
| Study Type | MS analysis |
| Study Summary | C57BL6 male mice were fed either a control 10% fat (RD D12540-B) or a high fat 60% fat (RD 12492) diet from 5 wk age. STZ was given to some groups at 12 wk age. Chow was reversed in some groups (DR) from 60% HF to 10 % HF at 16 wk age. Mice were euthanized at either 16 wk or 24 wk age. Neuropathy phenotyping occured prior to all harvests. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-05 |
| Num Groups | 11 |
| Total Subjects | 107 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000478 |
| Project DOI: | doi: 10.21228/M88G7V |
| Project Title: | Effect of high fat diet and streptozotocin treatment on neuropathy |
| Project Type: | MS analysis |
| Project Summary: | Ctrl,HF and DR mice: scn,drg,plasma for lipidomics |
| Institute: | University of Michigan |
| Department: | Neurology |
| Laboratory: | Feldman Lab |
| Last Name: | Feldman |
| First Name: | Eva |
| Address: | Ann Arbor, MI |
| Email: | efeldman@umich.edu |
| Phone: | 734-763-7274 |
Subject:
| Subject ID: | SU000699 |
| Subject Type: | Mouse |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | AGE |
|---|---|---|
| SA038687 | S00024314 | 16 week | DIET:Control | SAMPLE_TYPE:Dorsal root ganglia |
| SA038688 | S00024312 | 16 week | DIET:Control | SAMPLE_TYPE:Dorsal root ganglia |
| SA038689 | S00024320 | 16 week | DIET:Control | SAMPLE_TYPE:Dorsal root ganglia |
| SA038690 | S00024311 | 16 week | DIET:Control | SAMPLE_TYPE:Dorsal root ganglia |
| SA038691 | S00024315 | 16 week | DIET:Control | SAMPLE_TYPE:Dorsal root ganglia |
| SA038692 | S00024313 | 16 week | DIET:Control | SAMPLE_TYPE:Dorsal root ganglia |
| SA038693 | S00024316 | 16 week | DIET:Control | SAMPLE_TYPE:Dorsal root ganglia |
| SA038694 | S00024318 | 16 week | DIET:Control | SAMPLE_TYPE:Dorsal root ganglia |
| SA038695 | S00024319 | 16 week | DIET:Control | SAMPLE_TYPE:Dorsal root ganglia |
| SA038696 | S00024317 | 16 week | DIET:Control | SAMPLE_TYPE:Dorsal root ganglia |
| SA038697 | S00024395 | 16 week | DIET:Control | SAMPLE_TYPE:plasma |
| SA038698 | S00024394 | 16 week | DIET:Control | SAMPLE_TYPE:plasma |
| SA038699 | S00024392 | 16 week | DIET:Control | SAMPLE_TYPE:plasma |
| SA038700 | S00024396 | 16 week | DIET:Control | SAMPLE_TYPE:plasma |
| SA038701 | S00024393 | 16 week | DIET:Control | SAMPLE_TYPE:plasma |
| SA038702 | S00024391 | 16 week | DIET:Control | SAMPLE_TYPE:plasma |
| SA038703 | S00024400 | 16 week | DIET:Control | SAMPLE_TYPE:plasma |
| SA038704 | S00024399 | 16 week | DIET:Control | SAMPLE_TYPE:plasma |
| SA038705 | S00024398 | 16 week | DIET:Control | SAMPLE_TYPE:plasma |
| SA038706 | S00024397 | 16 week | DIET:Control | SAMPLE_TYPE:plasma |
| SA038766 | S00024235 | 16 week | DIET:control | SAMPLE_TYPE:Sciatic nerve |
| SA038767 | S00024234 | 16 week | DIET:control | SAMPLE_TYPE:Sciatic nerve |
| SA038768 | S00024232 | 16 week | DIET:control | SAMPLE_TYPE:Sciatic nerve |
| SA038769 | S00024236 | 16 week | DIET:control | SAMPLE_TYPE:Sciatic nerve |
| SA038770 | S00024233 | 16 week | DIET:control | SAMPLE_TYPE:Sciatic nerve |
| SA038771 | S00024240 | 16 week | DIET:control | SAMPLE_TYPE:Sciatic nerve |
| SA038772 | S00024237 | 16 week | DIET:control | SAMPLE_TYPE:Sciatic nerve |
| SA038773 | S00024239 | 16 week | DIET:control | SAMPLE_TYPE:Sciatic nerve |
| SA038774 | S00024231 | 16 week | DIET:control | SAMPLE_TYPE:Sciatic nerve |
| SA038775 | S00024238 | 16 week | DIET:control | SAMPLE_TYPE:Sciatic nerve |
| SA038736 | S00024336 | 16 week | DIET:HF | SAMPLE_TYPE:Dorsal root ganglia |
| SA038737 | S00024337 | 16 week | DIET:HF | SAMPLE_TYPE:Dorsal root ganglia |
| SA038738 | S00024338 | 16 week | DIET:HF | SAMPLE_TYPE:Dorsal root ganglia |
| SA038739 | S00024335 | 16 week | DIET:HF | SAMPLE_TYPE:Dorsal root ganglia |
| SA038740 | S00024334 | 16 week | DIET:HF | SAMPLE_TYPE:Dorsal root ganglia |
| SA038741 | S00024331 | 16 week | DIET:HF | SAMPLE_TYPE:Dorsal root ganglia |
| SA038742 | S00024332 | 16 week | DIET:HF | SAMPLE_TYPE:Dorsal root ganglia |
| SA038743 | S00024333 | 16 week | DIET:HF | SAMPLE_TYPE:Dorsal root ganglia |
| SA038744 | S00024340 | 16 week | DIET:HF | SAMPLE_TYPE:Dorsal root ganglia |
| SA038745 | S00024339 | 16 week | DIET:HF | SAMPLE_TYPE:Dorsal root ganglia |
| SA038756 | S00024416 | 16 week | DIET:HF | SAMPLE_TYPE:plasma |
| SA038757 | S00024418 | 16 week | DIET:HF | SAMPLE_TYPE:plasma |
| SA038758 | S00024420 | 16 week | DIET:HF | SAMPLE_TYPE:plasma |
| SA038759 | S00024413 | 16 week | DIET:HF | SAMPLE_TYPE:plasma |
| SA038760 | S00024412 | 16 week | DIET:HF | SAMPLE_TYPE:plasma |
| SA038761 | S00024417 | 16 week | DIET:HF | SAMPLE_TYPE:plasma |
| SA038762 | S00024415 | 16 week | DIET:HF | SAMPLE_TYPE:plasma |
| SA038763 | S00024414 | 16 week | DIET:HF | SAMPLE_TYPE:plasma |
| SA038764 | S00024419 | 16 week | DIET:HF | SAMPLE_TYPE:plasma |
| SA038765 | S00024411 | 16 week | DIET:HF | SAMPLE_TYPE:plasma |
| SA038746 | S00024255 | 16 week | DIET:HF | SAMPLE_TYPE:Sciatic nerve |
| SA038747 | S00024254 | 16 week | DIET:HF | SAMPLE_TYPE:Sciatic nerve |
| SA038748 | S00024253 | 16 week | DIET:HF | SAMPLE_TYPE:Sciatic nerve |
| SA038749 | S00024256 | 16 week | DIET:HF | SAMPLE_TYPE:Sciatic nerve |
| SA038750 | S00024251 | 16 week | DIET:HF | SAMPLE_TYPE:Sciatic nerve |
| SA038751 | S00024260 | 16 week | DIET:HF | SAMPLE_TYPE:Sciatic nerve |
| SA038752 | S00024259 | 16 week | DIET:HF | SAMPLE_TYPE:Sciatic nerve |
| SA038753 | S00024258 | 16 week | DIET:HF | SAMPLE_TYPE:Sciatic nerve |
| SA038754 | S00024252 | 16 week | DIET:HF | SAMPLE_TYPE:Sciatic nerve |
| SA038755 | S00024257 | 16 week | DIET:HF | SAMPLE_TYPE:Sciatic nerve |
| SA038707 | S00024358 | 16 week | DIET:HF STZ | SAMPLE_TYPE:Dorsal root ganglia |
| SA038708 | S00024354 | 16 week | DIET:HF STZ | SAMPLE_TYPE:Dorsal root ganglia |
| SA038709 | S00024353 | 16 week | DIET:HF STZ | SAMPLE_TYPE:Dorsal root ganglia |
| SA038710 | S00024360 | 16 week | DIET:HF STZ | SAMPLE_TYPE:Dorsal root ganglia |
| SA038711 | S00024351 | 16 week | DIET:HF STZ | SAMPLE_TYPE:Dorsal root ganglia |
| SA038712 | S00024355 | 16 week | DIET:HF STZ | SAMPLE_TYPE:Dorsal root ganglia |
| SA038713 | S00024352 | 16 week | DIET:HF STZ | SAMPLE_TYPE:Dorsal root ganglia |
| SA038714 | S00024359 | 16 week | DIET:HF STZ | SAMPLE_TYPE:Dorsal root ganglia |
| SA038715 | S00024357 | 16 week | DIET:HF STZ | SAMPLE_TYPE:Dorsal root ganglia |
| SA038716 | S00024356 | 16 week | DIET:HF STZ | SAMPLE_TYPE:Dorsal root ganglia |
| SA038727 | S00024434 | 16 week | DIET:HF STZ | SAMPLE_TYPE:plasma |
| SA038728 | S00024435 | 16 week | DIET:HF STZ | SAMPLE_TYPE:plasma |
| SA038729 | S00024436 | 16 week | DIET:HF STZ | SAMPLE_TYPE:plasma |
| SA038730 | S00024433 | 16 week | DIET:HF STZ | SAMPLE_TYPE:plasma |
| SA038731 | S00024431 | 16 week | DIET:HF STZ | SAMPLE_TYPE:plasma |
| SA038732 | S00024430 | 16 week | DIET:HF STZ | SAMPLE_TYPE:plasma |
| SA038733 | S00024429 | 16 week | DIET:HF STZ | SAMPLE_TYPE:plasma |
| SA038734 | S00024437 | 16 week | DIET:HF STZ | SAMPLE_TYPE:plasma |
| SA038735 | S00024432 | 16 week | DIET:HF STZ | SAMPLE_TYPE:plasma |
| SA038717 | S00024274 | 16 week | DIET:HF STZ | SAMPLE_TYPE:Sciatic nerve |
| SA038718 | S00024272 | 16 week | DIET:HF STZ | SAMPLE_TYPE:Sciatic nerve |
| SA038719 | S00024275 | 16 week | DIET:HF STZ | SAMPLE_TYPE:Sciatic nerve |
| SA038720 | S00024273 | 16 week | DIET:HF STZ | SAMPLE_TYPE:Sciatic nerve |
| SA038721 | S00024278 | 16 week | DIET:HF STZ | SAMPLE_TYPE:Sciatic nerve |
| SA038722 | S00024271 | 16 week | DIET:HF STZ | SAMPLE_TYPE:Sciatic nerve |
| SA038723 | S00024280 | 16 week | DIET:HF STZ | SAMPLE_TYPE:Sciatic nerve |
| SA038724 | S00024279 | 16 week | DIET:HF STZ | SAMPLE_TYPE:Sciatic nerve |
| SA038725 | S00024276 | 16 week | DIET:HF STZ | SAMPLE_TYPE:Sciatic nerve |
| SA038726 | S00024277 | 16 week | DIET:HF STZ | SAMPLE_TYPE:Sciatic nerve |
| SA038776 | S00024327 | 24 week | DIET:Control | SAMPLE_TYPE:Dorsal root ganglia |
| SA038777 | S00024328 | 24 week | DIET:Control | SAMPLE_TYPE:Dorsal root ganglia |
| SA038778 | S00024329 | 24 week | DIET:Control | SAMPLE_TYPE:Dorsal root ganglia |
| SA038779 | S00024326 | 24 week | DIET:Control | SAMPLE_TYPE:Dorsal root ganglia |
| SA038780 | S00024330 | 24 week | DIET:Control | SAMPLE_TYPE:Dorsal root ganglia |
| SA038781 | S00024325 | 24 week | DIET:Control | SAMPLE_TYPE:Dorsal root ganglia |
| SA038782 | S00024322 | 24 week | DIET:Control | SAMPLE_TYPE:Dorsal root ganglia |
| SA038783 | S00024321 | 24 week | DIET:Control | SAMPLE_TYPE:Dorsal root ganglia |
| SA038784 | S00024323 | 24 week | DIET:Control | SAMPLE_TYPE:Dorsal root ganglia |
| SA038785 | S00024324 | 24 week | DIET:Control | SAMPLE_TYPE:Dorsal root ganglia |
| SA038786 | S00024403 | 24 week | DIET:Control | SAMPLE_TYPE:plasma |
Collection:
| Collection ID: | CO000693 |
| Collection Summary: | - |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR000713 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000706 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A004_-_Shotgun_Lipidomics.pdf |
Combined analysis:
| Analysis ID | AN001038 | AN001039 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Shimadzu CTO-20A Nexera X2 | Shimadzu CTO-20A Nexera X2 |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple TOF | Triple TOF |
| MS instrument name | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area normalized | peak area normalized |
Chromatography:
| Chromatography ID: | CH000739 |
| Instrument Name: | Shimadzu CTO-20A Nexera X2 |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.400uL/min |
| Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000933 |
| Analysis ID: | AN001038 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| MS ID: | MS000934 |
| Analysis ID: | AN001039 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
