Summary of Study ST000699
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000496. The data can be accessed directly via it's Project DOI: 10.21228/M8Z32C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000699 |
| Study Title | Human vitreous amino acid & lipid analysis |
| Study Type | MS analysis |
| Study Summary | Human vitreous was collected in the OR at time of indicated surgery. No treatments were involved. Diagnoses are: epiretinal membranes (ERM); proliferative diabetic retinopathy (PDR); retinal detachment (RD) |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-19 |
| Num Groups | 3 |
| Total Subjects | 3 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000496 |
| Project DOI: | doi: 10.21228/M8Z32C |
| Project Title: | Comparative Vitreous Analysis |
| Project Type: | MS analysis |
| Project Summary: | Human vitreous collected in OR |
| Institute: | University of Michigan |
| Department: | Ophthalmology |
| Laboratory: | Gardner |
| Last Name: | Gardner |
| First Name: | Thomas |
| Address: | Ann Arbor, MI |
| Email: | tomwgard@umich.edu |
| Phone: | 734-232-8283 |
Subject:
| Subject ID: | SU000722 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Diagnosis |
|---|---|---|
| SA039482 | S00018825 | ERM |
| SA039483 | S00018821 | ERM |
| SA039484 | S00018824 | ERM |
| SA039485 | S00018823 | ERM |
| SA039486 | S00018822 | ERM |
| SA039487 | S00018829 | PDR |
| SA039488 | S00018828 | PDR |
| SA039489 | S00018827 | PDR |
| SA039490 | S00018826 | PDR |
| SA039491 | S00018833 | RD |
| SA039492 | S00018834 | RD |
| SA039493 | S00018832 | RD |
| SA039494 | S00018830 | RD |
| SA039495 | S00018831 | RD |
| Showing results 1 to 14 of 14 |
Collection:
| Collection ID: | CO000716 |
| Collection Summary: | - |
| Sample Type: | Vitreous |
Treatment:
| Treatment ID: | TR000736 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000729 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A004_-_Shotgun_Lipidomics.pdf |
Combined analysis:
| Analysis ID | AN001085 | AN001086 | AN001087 |
|---|---|---|---|
| Chromatography ID | CH000763 | CH000763 | CH000764 |
| MS ID | MS000980 | MS000981 | MS000982 |
| Analysis type | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | GC |
| Chromatography system | Shimadzu CTO-20A Nexera X2 | Shimadzu CTO-20A Nexera X2 | Agilent 7890N |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Phenomenex Zebron ZB-AAA Amino Acid Analysis 10m x 0.25mm |
| MS Type | ESI | ESI | EI |
| MS instrument type | Triple TOF | Triple TOF | Single quadrupole |
| MS instrument name | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF | Agilent 5975 |
| Ion Mode | NEGATIVE | POSITIVE | POSITIVE |
| Units | peak area normalized | peak area normalized | uM |
Chromatography:
| Chromatography ID: | CH000763 |
| Instrument Name: | Shimadzu CTO-20A Nexera X2 |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.400uL/min |
| Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000764 |
| Instrument Name: | Agilent 7890N |
| Column Name: | Phenomenex Zebron ZB-AAA Amino Acid Analysis 10m x 0.25mm |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000980 |
| Analysis ID: | AN001085 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| MS ID: | MS000981 |
| Analysis ID: | AN001086 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| MS ID: | MS000982 |
| Analysis ID: | AN001087 |
| Instrument Name: | Agilent 5975 |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A005_Amino_Acids.pdf |
