Summary of Study ST000732

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000529. The data can be accessed directly via it's Project DOI: 10.21228/M89101 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000732
Study TitleAcylcarnitine profile in fast-refed livers
Study TypeMS analysis
Study SummaryThe aim of this study is to determine whether changes in CoA degradation affect the composition of the acylcarntine pool in the liver. 7 wild-type and 7 Nudt7 knockout male mice (10-16 weeks of age) on a mixed background were fasted for 24h and allowed to refeed for 12-14hours before removing the liver. The liver samples were flash-frozen and stored at -80C until used.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups4
Total Subjects4
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M89101
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000529
Project DOI:doi: 10.21228/M89101
Project Title:Determining the role of Nudt7 in liver metabolism
Project Type:MS analysis
Project Summary:Determining the role of Nudt7 in liver metabolism
Institute:West Virginia University
Department:Biochemistry and Molecular Biology
Laboratory:Leonardi Lab
Last Name:Leonardi
First Name:Roberta
Address:Morgantown, WV
Email:roleonardi@hsc.wvu.edu
Phone:304-293-7591

Subject:

Subject ID:SU000755
Subject Type:MOUSE
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: MOUSE; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Hours after start of re-feeding
SA040936S0002868312h | Nudt7 Genotype:KO
SA040937S0002868412h | Nudt7 Genotype:KO
SA040938S0002868212h | Nudt7 Genotype:WT
SA040939S0002868112h | Nudt7 Genotype:WT
SA040940S0002869214h | Nudt7 Genotype:KO
SA040941S0002869014h | Nudt7 Genotype:KO
SA040942S0002869414h | Nudt7 Genotype:KO
SA040943S0002869114h | Nudt7 Genotype:KO
SA040944S0002869314h | Nudt7 Genotype:KO
SA040945S0002868814h | Nudt7 Genotype:WT
SA040946S0002868514h | Nudt7 Genotype:WT
SA040947S0002868614h | Nudt7 Genotype:WT
SA040948S0002868714h | Nudt7 Genotype:WT
SA040949S0002868914h | Nudt7 Genotype:WT
Showing results 1 to 14 of 14

Collection:

Collection ID:CO000749
Collection Summary:-
Sample Type:Liver

Treatment:

Treatment ID:TR000769
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000762
Sampleprep Summary:-
Sampleprep Protocol Filename:A008_-_Acyl_Carnitines.pdf

Combined analysis:

Analysis ID AN001146
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490A QQQ
Ion Mode POSITIVE
Units nmol/mg

Chromatography:

Chromatography ID:CH000806
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Column Temperature:40
Flow Rate:0.250 mL/min
Solvent A:100% water; 5 mM ammonium acetate, pH 9.9
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS001041
Analysis ID:AN001146
Instrument Name:Agilent 6490A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A008_-_Acyl_Carnitines.pdf
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