Summary of Study ST001774

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001128. The data can be accessed directly via it's Project DOI: 10.21228/M8WQ4Q This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001774
Study TitleTimecourse on primary human highly differentiated airway epithelial cells infected with HRV-C15
Study SummaryAt each time point (4, 12, 24 h), ALI basolateral media was collected and incubated 1:1 in ice-cold 100% methanol for 30 minutes on ice, vortexing every 10 minutes. Basolateral media was centrifuged at 20,000 × 𝑔 for 10 minutes at 4°C, and further diluted in 50% methanol prior to mass spectrometry plate loading.
Institute
McGill University
Last NameLopes
First NameFernando
Address21111 Lakeshore Rd
Emailfernando.lopes@mcgill.ca
Phone5143987607
Submit Date2021-05-03
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2021-05-12
Release Version1
Fernando Lopes Fernando Lopes
https://dx.doi.org/10.21228/M8WQ4Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001128
Project DOI:doi: 10.21228/M8WQ4Q
Project Title:PGC-1 alpha mediates a metabolic host defense response in human airway epithelium during rhinovirus infections
Project Summary:Quantitative Metabolomics studies on samples from human airway epithelial cell cultures
Institute:McGill University
Last Name:Fernando Lopes
First Name:Fernando
Address:21111 Lakeshore Rd, Ste-Anne-de-Bellevue, Quebec, H9X 3V9, Canada
Email:fernando.lopes@mcgill.ca
Phone:5143987607

Subject:

Subject ID:SU001851
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Not applicable
Species Group:Mammals

Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Experimental variable
SA1645232020_04_15_AM_HLICNeg15_HBE84_12_CControl 12h
SA1645242020_04_15_AM_HLICNeg15_HBE85_12_CControl 12h
SA1645252020_04_15_AM_HLICNeg15_HBE91_4_CControl 12h
SA1645262020_04_15_AM_HLICNeg15_HBE82_12_CControl 12h
SA1645272020_04_15_AM_HLICNeg15_HBE90_12_CControl 12h
SA1645282020_04_15_AM_HLICNeg15_HBE58_12_CControl 12h
SA1645292020_04_15_AM_HLICNeg15_HBE85_24_CControl 24h
SA1645302020_04_15_AM_HLICNeg15_HBE91_12_CControl 24h
SA1645312020_04_15_AM_HLICNeg15_HBE58_24_CControl 24h
SA1645322020_04_15_AM_HLICNeg15_HBE90_24_CControl 24h
SA1645332020_04_15_AM_HLICNeg15_HBE84_24_CControl 24h
SA1645342020_04_15_AM_HLICNeg15_HBE82_24_CControl 24h
SA1645352020_04_15_AM_HLICNeg15_HBE82_4_CControl 4h
SA1645362020_04_15_AM_HLICNeg15_HBE91_24_CControl 4h
SA1645372020_04_15_AM_HLICNeg15_HBE90_4_CControl 4h
SA1645382020_04_15_AM_HLICNeg15_HBE84_4_CControl 4h
SA1645392020_04_15_AM_HLICNeg15_HBE58_4_CControl 4h
SA1645402020_04_15_AM_HLICNeg15_HBE85_4_CControl 4h
SA1645412020_04_15_AM_HLICNeg15_HBE84_12_VHRV-C15 infection 12h
SA1645422020_04_15_AM_HLICNeg15_HBE90_12_VHRV-C15 infection 12h
SA1645432020_04_15_AM_HLICNeg15_Media_12_VHRV-C15 infection 12h
SA1645442020_04_15_AM_HLICNeg15_HBE58_12_VHRV-C15 infection 12h
SA1645452020_04_15_AM_HLICNeg15_HBE85_12_VHRV-C15 infection 12h
SA1645462020_04_15_AM_HLICNeg15_HBE91_12_VHRV-C15 infection 12h
SA1645472020_04_15_AM_HLICNeg15_HBE82_12_VHRV-C15 infection 12h
SA1645482020_04_15_AM_HLICNeg15_HBE85_24_VHRV-C15 infection 24h
SA1645492020_04_15_AM_HLICNeg15_HBE82_24_VHRV-C15 infection 24h
SA1645502020_04_15_AM_HLICNeg15_HBE84_24_VHRV-C15 infection 24h
SA1645512020_04_15_AM_HLICNeg15_HBE91_24_VHRV-C15 infection 24h
SA1645522020_04_15_AM_HLICNeg15_Media_24_VHRV-C15 infection 24h
SA1645532020_04_15_AM_HLICNeg15_HBE58_24_VHRV-C15 infection 24h
SA1645542020_04_15_AM_HLICNeg15_HBE90_24_VHRV-C15 infection 24h
SA1645552020_04_15_AM_HLICNeg15_HBE91_4_VHRV-C15 infection 4h
SA1645562020_04_15_AM_HLICNeg15_HBE90_4_VHRV-C15 infection 4h
SA1645572020_04_15_AM_HLICNeg15_HBE58_4_VHRV-C15 infection 4h
SA1645582020_04_15_AM_HLICNeg15_HBE85_4_VHRV-C15 infection 4h
SA1645592020_04_15_AM_HLICNeg15_Media_4_VHRV-C15 infection 4h
SA1645602020_04_15_AM_HLICNeg15_HBE82_4_VHRV-C15 infection 4h
SA1645612020_04_15_AM_HLICNeg15_HBE84_4_VHRV-C15 infection 4h
SA1645622020_04_15_AM_HLICNeg15_Media_FMedia
SA1645632020_04_15_AM_HLICNeg15_Media_12Media 12h in the incubator
SA1645642020_04_15_AM_HLICNeg15_Media_24Media 24h in the incubator
SA1645652020_04_15_AM_HLICNeg15_Media_4Media 4h in the incubator
SA1645662020_04_15_AM_HLICNeg15_Midblank1Midblank
SA1645672020_04_15_AM_HLICNeg15_Postplank1Postplan
SA1645682020_04_15_AM_HLICNeg15_Postplank3Postplan
SA1645692020_04_15_AM_HLICNeg15_Postplank2Postplan
SA1645702020_04_15_AM_HLICNeg15_Preblank1Preblank
SA1645712020_04_15_AM_HLICNeg15_Preblank2Preblank
SA1645722020_04_15_AM_HLICNeg15_Preblank3Preblank
Showing results 1 to 50 of 50

Collection:

Collection ID:CO001844
Collection Summary:At each time point (4, 12, 24 h), ALI basolateral media was collected and incubated 1:1 in ice-cold 100% methanol for 30 minutes on ice, vortexing every 10 minutes. Basolateral media was centrifuged at 20,000 × 𝑔 for 10 minutes at 4°C, and further diluted in 50% methanol prior to mass spectrometry plate loading.
Sample Type:Cultured cells

Treatment:

Treatment ID:TR001864
Treatment Summary:Highly differentiated primary human airway epithelial were infected or not with HRV-C15 for 4, 12 or 24 h.
Treatment Compound:Human rhinovirus C15

Sample Preparation:

Sampleprep ID:SP001857
Sampleprep Summary:media was collected and incubated 1:1 in ice-cold 100% methanol for 30 minutes on ice, vortexing every 10 minutes. Basolateral media was centrifuged at 20,000 × 𝑔 for 10 minutes at 4°C, and further diluted in 50% methanol prior to mass spectrometry plate loading.

Combined analysis:

Analysis ID AN002881
Chromatography ID CH002136
MS ID MS002674
Analysis type MS
Chromatography type HILIC
Chromatography system Thermo Vanquish
Column ThermoFisher Syncronis HILIC UHPLC (100 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Single quadrupole
MS instrument name Thermo Q Exactive HF hybrid Orbitrap
Ion Mode NEGATIVE
Units pmoles/l

Chromatography:

Chromatography ID:CH002136
Chromatography Summary:General metabolomic run were performed on a Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ Mass Spectrometer (ThermoFisher) coupled to a Vanquish™ UHPLC System (ThermoFisher). Chromatographic separation was achieved on a Syncronis HILIC UHPLC column (2.1 mm x 100 mm x 1.7 mm, ThermoFisher) using a binary solvent system at a flow rate of 600 mL/min. Solvent A, 20 mM ammonium formate pH 3.0 in mass spectrometry grade H2O; Solvent B, mass spectrometry grade acetonitrile with 0.1% formic acid (% v/v). A sample injection volume of 2 mL was used.
Instrument Name:Thermo Vanquish
Column Name:ThermoFisher Syncronis HILIC UHPLC (100 x 2.1mm,1.7um)
Solvent A:100% water; 20 mM ammonium formate, pH 3
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:HILIC

MS:

MS ID:MS002674
Analysis ID:AN002881
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Single quadrupole
MS Type:ESI
MS Comments:The mass spectrometer was run in negative full scan mode at a resolution of 240,000 scanning from 50-750 m/z.
Ion Mode:NEGATIVE
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