Summary of Study ST002022
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001284. The data can be accessed directly via it's Project DOI: 10.21228/M8R71H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002022 |
| Study Title | A Sentinel Serum Quality Management Program for NMR Metabolomics |
| Study Type | Pharmacometabolomics |
| Study Summary | Pooled human serum (Sentinel Serum Samples) was aliquoted for storage at -80C and subsequently had NMR spectra acquired annually for three years. This was to determine sentinel serum sample utility for a quality management program to monitor NMR reproducibility. Sentinel serum quality control samples allowed the quantification of variance between different profilers within a project. They also demonstrated declines in metabolite concentrations over time. A sentinel program is useful for tracking changes in QC serum samples from long-term storage. Separately, QC samples show negligible variance introduced by multiple profilers of NMR spectra within a project. |
| Institute | University of Michigan |
| Department | Clinical Pharmacy |
| Laboratory | UMich NMR Metabolomics Laboratory |
| Last Name | Stringer |
| First Name | Kathleen |
| Address | 428 Church Street |
| stringek@med.umich.edu; stringek@umich.edu | |
| Phone | 7346474775 |
| Submit Date | 2021-11-30 |
| Num Groups | 3 |
| Total Subjects | 1 |
| Study Comments | Samples were aliquots of a batch of pooled human serum, collected from multiple different individuals |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | NMR |
| Release Date | 2022-01-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001284 |
| Project DOI: | doi: 10.21228/M8R71H |
| Project Title: | A Sentinel Serum Quality Management Program for NMR Metabolomics |
| Project Type: | Metabolomics |
| Project Summary: | Pooled human serum (Sentinel Serum Samples) was aliquoted for storage at -80C and subsequently had NMR spectra acquired annually for three years. This was to determine sentinel serum sample utility for a quality management program to monitor NMR reproducibility. Sentinel serum quality control samples allowed the quantification of variance between different profilers within a project. They also demonstrated declines in metabolite concentrations over time. A sentinel program is useful for tracking changes in QC serum samples from long-term storage. Separately, QC samples show negligible variance introduced by multiple profilers of NMR spectra within a project. |
| Institute: | University of Michigan |
| Department: | Clinical Pharmacy |
| Laboratory: | UMich NMR Metabolomics Laboratory |
| Last Name: | Stringer |
| First Name: | Kathleen |
| Address: | 428 Church Street |
| Email: | stringek@med.umich.edu; stringek@umich.edu |
| Phone: | 7346474775 |
| Funding Source: | NIH/NIGMS |
| Contributors: | T. Flott, K Stringer |
Subject:
| Subject ID: | SU002104 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Year |
|---|---|---|
| SA189410 | 236_001 | 1 |
| SA189411 | 236_010 | 1 |
| SA189412 | 236_012 | 1 |
| SA189413 | 236_008 | 1 |
| SA189414 | 236_011 | 1 |
| SA189415 | 236_009 | 1 |
| SA189416 | 236_003 | 1 |
| SA189417 | 236_007 | 1 |
| SA189418 | 236_004 | 1 |
| SA189419 | 236_002 | 1 |
| SA189420 | 236_005 | 1 |
| SA189421 | 236_006 | 1 |
| SA189422 | 236_021 | 2 |
| SA189423 | 236_020 | 2 |
| SA189424 | 236_023 | 2 |
| SA189425 | 236_024 | 2 |
| SA189426 | 236_022 | 2 |
| SA189427 | 236_019 | 2 |
| SA189428 | 236_014 | 2 |
| SA189429 | 236_018 | 2 |
| SA189430 | 236_015 | 2 |
| SA189431 | 236_013 | 2 |
| SA189432 | 236_017 | 2 |
| SA189433 | 236_016 | 2 |
| SA189434 | 236_033 | 3 |
| SA189435 | 236_034 | 3 |
| SA189436 | 236_035 | 3 |
| SA189437 | 236_032 | 3 |
| SA189438 | 236_036 | 3 |
| SA189439 | 236_028 | 3 |
| SA189440 | 236_026 | 3 |
| SA189441 | 236_025 | 3 |
| SA189442 | 236_027 | 3 |
| SA189443 | 236_029 | 3 |
| SA189444 | 236_030 | 3 |
| SA189445 | 236_031 | 3 |
| Showing results 1 to 36 of 36 |
Collection:
| Collection ID: | CO002097 |
| Collection Summary: | Samples were aliquoted from pooled sera collected from multiple individuals. Samples were purchased from Innovative Research (Novi, MI). |
| Sample Type: | Blood (serum) |
| Storage Conditions: | -80℃ |
| Storage Vials: | Wheaton 2mL Cryovials |
| Additives: | Na Heparin |
Treatment:
| Treatment ID: | TR002116 |
| Treatment Summary: | Samples were not treated, beyond the initial aliquotting of the pooled human sera upon receipt at our laboratory before storage at -80C |
Sample Preparation:
| Sampleprep ID: | SP002110 |
| Sampleprep Summary: | Prior to spectral acquisition samples were methanol precipitated and ultrafiltered to remove macromolecules prior to spectra acquisition |
| Sampleprep Protocol Filename: | Serum_Precipitation_SOP.docx, Serum_Filtration_SOP.docx |
| Processing Storage Conditions: | On ice |
| Extraction Method: | Methanol Precipitation |
| Extract Storage: | -80℃ |
| Sample Resuspension: | Samples were resuspended in 50mM phosphate buffer in D2O |
| Sample Spiking: | Samples were spiked with a Formate solution in D2O of known concentration |
Analysis:
| Analysis ID: | AN003292 |
| Analysis Type: | NMR |
| Num Factors: | 3 |
| Num Metabolites: | 31 |
| Units: | uM |
NMR:
| NMR ID: | NM000226 |
| Analysis ID: | AN003292 |
| Instrument Name: | Varian 500 MHz |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Spectrometer Frequency: | 500 |
| NMR Solvent: | D2O |
| NMR Tube Size: | 5mm |
| Shimming Method: | Auto shim (gradient shimming), with manual shimming when necessary |
| Pulse Sequence: | 1D-NOESY |
| Water Suppression: | Saturation at 80Hz induced field strength |
| Temperature: | 25 |
| Number Of Scans: | 32 |
