Summary of Study ST002049

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001296. The data can be accessed directly via it's Project DOI: 10.21228/M86697 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002049
Study TitlePerfluoroalkyl Compounds and Child Metabolic Health - Healthy Start Cohort
Study TypeUntargeted LC-MS Metabolomics Study
Study SummaryHealthy Start is a prospective, pre-birth cohort study that recruited pregnant participants from outpatient prenatal clinics at the University of Colorado Hospital between 2009 and 2014. Eligible participants were 16 years or older with singleton pregnancies, no history of stillbirth or extremely preterm birth (<25 weeks of gestation) and no serious medical conditions, and had not yet completed 24 weeks of gestation at the time of enrollment. Mothers completed two study visits during pregnancy (median gestational ages 17 and 27 weeks). Mother-child pairs were thereafter assessed at birth, and for the child’s follow up, in mid-childhood (median age 4.8 years). For the present project, we will use data from 523 mother-child pairs of the Healthy Start cohort with available information on prenatal PFAS concentrations, available cord plasma samples at delivery, and outcomes of interest. Please contact Wei Perng at wei.perng@cuanschutz.edu for questions related to the subject characteristics and outcomes. This research was supported by the Environmental influences on Child Health Outcomes (ECHO) program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Healthy Start is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375 ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Parker CB; U24 OD023382 North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539
Institute
University of Colorado Anschutz Medical Campus
DepartmentAnschutz Medical Campus
Last NamePerng
First NameWei
Address12474 East 19th Ave, Room 208, Aurora, CO 80045
EmailWei.Perng@CUAnschutz.edu
Phone734-717-0982
Submit Date2021-12-22
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-12-22
Release Version1
Wei Perng Wei Perng
https://dx.doi.org/10.21228/M86697
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001296
Project DOI:doi: 10.21228/M86697
Project Title:Perfluoroalkyl Compounds and Child Metabolic Health (Healthy Start Cohort)
Project Type:Metabolomics
Project Summary:The project Perfluoroalkyl Compounds and Child Metabolic Health is funded by the NIH Environmental influences on Child Health Outcomes (ECHO) program, and uses data from the Healthy Start and Project Viva ECHO cohorts. This project will examine associations of untargeted metabolomics data acquired from cord blood with prenatal PFAS exposure and the effect on infant/child metabolic health.
Institute:NC HHEAR Hub
Department:Untargeted Analysis
Laboratory:Sumner/Li Lab
Last Name:Sumner, Li
First Name:Susan, Yuan
Address:500 Laureate Way, Kannapolis, NC 28081
Email:susan_sumner@unc.edu, yuanyli4@unc.edu
Phone:9196224456
Funding Source:This research was supported by the Environmental influences on Child Health Outcomes (ECHO) program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Healthy Start is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375 ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Parker CB; U24 OD023382 North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539

Subject:

Subject ID:SU002131
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Male and female

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Stuy type
SA192633HHEAR_24HHEAR reference material
SA192634HHEAR_25HHEAR reference material
SA192635HHEAR_26HHEAR reference material
SA192636HHEAR_23HHEAR reference material
SA192637HHEAR_22HHEAR reference material
SA192638HHEAR_20HHEAR reference material
SA192639HHEAR_21HHEAR reference material
SA192640HHEAR_27HHEAR reference material
SA192641HHEAR_29HHEAR reference material
SA192642HHEAR_33HHEAR reference material
SA192643HHEAR_34HHEAR reference material
SA192644HHEAR_35HHEAR reference material
SA192645HHEAR_32HHEAR reference material
SA192646HHEAR_31HHEAR reference material
SA192647HHEAR_19HHEAR reference material
SA192648HHEAR_30HHEAR reference material
SA192649HHEAR_28HHEAR reference material
SA192650HHEAR_17HHEAR reference material
SA192651HHEAR_5HHEAR reference material
SA192652HHEAR_6HHEAR reference material
SA192653HHEAR_7HHEAR reference material
SA192654HHEAR_4HHEAR reference material
SA192655HHEAR_3HHEAR reference material
SA192656HHEAR_1HHEAR reference material
SA192657HHEAR_2HHEAR reference material
SA192658HHEAR_8HHEAR reference material
SA192659HHEAR_9HHEAR reference material
SA192660HHEAR_15HHEAR reference material
SA192661HHEAR_16HHEAR reference material
SA192662HHEAR_36HHEAR reference material
SA192663HHEAR_14HHEAR reference material
SA192664HHEAR_13HHEAR reference material
SA192665HHEAR_11HHEAR reference material
SA192666HHEAR_12HHEAR reference material
SA192667HHEAR_18HHEAR reference material
SA192668HHEAR_10HHEAR reference material
SA192669HHEAR_50HHEAR reference material
SA192670HHEAR_48HHEAR reference material
SA192671HHEAR_47HHEAR reference material
SA192672HHEAR_51HHEAR reference material
SA192673HHEAR_52HHEAR reference material
SA192674HHEAR_37HHEAR reference material
SA192675HHEAR_53HHEAR reference material
SA192676HHEAR_46HHEAR reference material
SA192677HHEAR_49HHEAR reference material
SA192678HHEAR_40HHEAR reference material
SA192679HHEAR_45HHEAR reference material
SA192680HHEAR_38HHEAR reference material
SA192681HHEAR_41HHEAR reference material
SA192682HHEAR_39HHEAR reference material
SA192683HHEAR_42HHEAR reference material
SA192684HHEAR_43HHEAR reference material
SA192685HHEAR_44HHEAR reference material
SA192686NIST_13NIST reference material
SA192687NIST_11NIST reference material
SA192688NIST_12NIST reference material
SA192689NIST_15NIST reference material
SA192690NIST_17NIST reference material
SA192691NIST_10NIST reference material
SA192692NIST_18NIST reference material
SA192693NIST_16NIST reference material
SA192694NIST_14NIST reference material
SA192695NIST_3NIST reference material
SA192696NIST_2NIST reference material
SA192697NIST_1NIST reference material
SA192698NIST_5NIST reference material
SA192699NIST_4NIST reference material
SA192700NIST_9NIST reference material
SA192701NIST_7NIST reference material
SA192702NIST_8NIST reference material
SA192703NIST_6NIST reference material
SA192704S_379Study sample
SA192705S_380Study sample
SA192706S_378Study sample
SA192707S_376Study sample
SA192708S_377Study sample
SA192709S_381Study sample
SA192710S_386Study sample
SA192711S_387Study sample
SA192712S_388Study sample
SA192713S_374Study sample
SA192714S_385Study sample
SA192715S_383Study sample
SA192716S_384Study sample
SA192717S_382Study sample
SA192718S_370Study sample
SA192719S_364Study sample
SA192720S_365Study sample
SA192721S_363Study sample
SA192722S_362Study sample
SA192723S_389Study sample
SA192724S_366Study sample
SA192725S_367Study sample
SA192726S_371Study sample
SA192727S_372Study sample
SA192728S_361Study sample
SA192729S_369Study sample
SA192730S_368Study sample
SA192731S_373Study sample
SA192732S_408Study sample
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Collection:

Collection ID:CO002124
Collection Summary:Healthy Start is a prospective, pre-birth cohort study that recruited pregnant participants from outpatient prenatal clinics at the University of Colorado Hospital between 2009 and 2014. Eligible participants were 16 years or older with singleton pregnancies, no history of stillbirth or extremely preterm birth (<25 weeks of gestation) and no serious medical conditions, and had not yet completed 24 weeks of gestation at the time of enrollment. Mothers completed two study visits during pregnancy (median gestational ages 17 and 27 weeks). Mother-child pairs were thereafter assessed at birth, and for the child’s follow up, in mid-childhood (median age 4.8 years). For the present project, we will use data from 523 mother-child pairs of the Healthy Start cohort with available information on prenatal PFAS concentrations, available cord plasma samples at delivery, and outcomes of interest.
Sample Type:Blood (serum)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002143
Treatment Summary:N/A

Sample Preparation:

Sampleprep ID:SP002137
Sampleprep Summary:Study serum samples (50 µL each) and total study pool samples (50 µL each) were prepared and shipped from the LEAD Center at the University of Colorado Denver Anschutz Medical Campus to the NC HHEAR Hub on dry ice. Aliquots of HHEAR reference plasma (50 µL each) and NIST serum (909C) reference material (50 µL each) were provided by the NC HHEAR Hub. All samples were stored under -80° C until lab analysis. All samples were thawed at 4 °C overnight a day before sample preparation. The same volume (50 µL) of the LC-MS grade water was used as blank. Samples, including study samples, total study pool samples, HHEAR reference material, NIST reference material, and blanks were mixed with 400 µL methanol containing 500 ng/mL tryptophan-d5 as internal standard and vortexed by the multiple tube vortex mixer for 2 min at 5000 rpm in room temperature. All samples were centrifuged at 16, 000 rcf for 10 min at 4°C. The supernatant (350 µL) was transferred into a pre-labeled 2.0 mL Lo-bind Eppendorf tube, and then dried by a SpeedVac overnight. For immediate analysis, 100 µL of water-methanol solution (95:5, v/v) was used to reconstitute the dried extracts, and the samples were thoroughly mixed on the multiple tube vortex mixer for 10 min at 5000 rpm in room temperature. All samples were centrifuged at 4°C for 10 min at 16,000 rcf. The supernatant was transferred to pre-labeled autosampler vials for data acquisition by LC-MS.
Processing Storage Conditions:On ice
Extract Storage:Described in summary

Combined analysis:

Analysis ID AN003336
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Scientific™ Vanquish™ UPHPLC
Column Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF hybrid Orbitrap
Ion Mode POSITIVE
Units Normalized intensity

Chromatography:

Chromatography ID:CH002470
Chromatography Summary:Reverse phase
Instrument Name:Thermo Scientific™ Vanquish™ UPHPLC
Column Name:Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um)
Column Pressure:6000-10000
Column Temperature:50
Flow Gradient:Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 6. 22.00 0.4 99.0 1.0 5
Flow Rate:0.4 ml/min
Injection Temperature:8
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Randomization Order:Randomized
Chromatography Type:Reversed phase

MS:

MS ID:MS003105
Analysis ID:AN003336
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Instrument: Thermo Q Exactive HFx Software: Xcalibur 4.1.31.9 for data acquisition; Progenesis QI 2.1 for data preprocessing
Ion Mode:POSITIVE
Capillary Temperature:320 °C
Capillary Voltage:3.5 KV
Collision Energy:20-45, ramp
Collision Gas:N2
Dry Gas Flow:55
Dry Gas Temp:400°C
Fragmentation Method:CID
Desolvation Gas Flow:55
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