Summary of Study ST002081

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001321. The data can be accessed directly via it's Project DOI: 10.21228/M8ZM5P This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002081
Study TitleDynamic Lipidome Alterations Associated with Human Health, Disease, and Aging
Study SummaryLipids comprise a complex mixture of molecules that play central but undercharacterized roles across a wide range of functions such as cell membrane maintenance, energy management, and cell signaling. Here, we describe a comprehensive longitudinal lipidomic profiling approach aiming to provide new physiological insights into aging, diabetes, inflammation, and cytokine regulations. By profiling the plasma lipidome to a depth of more than 800 lipid species across 1,546 samples collected from 109 subjects spanning up to 9 years (3.2 average), we identified a myriad of dysregulated lipid species highly associated with transitions from health to disease. Our data suggest distinct physiological roles of complex lipid subclasses including large and small triacylglycerols (TAG), ester- and ether-linked phosphatidylethanolamines (PE), lysophosphatidylcholines (LPC), and lysophosphatidylethanolamine (LPE). The dynamic changes in the plasma lipidome under the conditions of respiratory viral infections, insulin resistance (IR), and aging indicate a putative role of these different lipids in regulating immune homeostasis in health as well as in acute and chronic inflammation. Moreover, metabolically unhealthy subjects diagnosed with IR show (1) disturbed immune homeostasis and differences in specific lipid-clinical measure associations, (2) altered dynamics for particular complex lipids including TAGs, LPCs PEs, and PCs in response to acute infections, and (3) elevated levels of complex lipids such as TAGs and CEs and accelerated aging, highlighting the importance of context specific interpretation of lipid profiles. Overall, our study exemplifies the power of deep and quantitative lipidomics profiling in conjunction with other omics measures to provide new insights into lipidome dynamics in health and disease.
Institute
Stanford University
Last NameHornburg
First NameDaniel
AddressDepartment of Genetics, Stanford University School of Medicine, Stanford, CA, 94305, USA
Emaildaniel.dh.hornburg@gmail.com
Phone650-736-8099
Submit Date2022-10-13
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailMS(Dir. Inf.)
Release Date2023-05-05
Release Version1
Daniel Hornburg Daniel Hornburg
https://dx.doi.org/10.21228/M8ZM5P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001321
Project DOI:doi: 10.21228/M8ZM5P
Project Title:Dynamic Lipidome Alterations Associated with Human Health, Disease, and Aging
Project Type:Multi-omics
Project Summary:Lipids comprise a complex mixture of molecules that play central but undercharacterized roles across a wide range of functions such as cell membrane maintenance, energy management, and cell signaling. Here, we describe a comprehensive longitudinal lipidomic profiling approach aiming to provide new physiological insights into aging, diabetes, inflammation, and cytokine regulations. By profiling the plasma lipidome to a depth of more than 800 lipid species across 1,546 samples collected from 109 subjects spanning up to 9 years (3.2 average), we identified a myriad of dysregulated lipid species highly associated with transitions from health to disease. Our data suggest distinct physiological roles of complex lipid subclasses including large and small triacylglycerols (TAG), ester- and ether-linked phosphatidylethanolamines (PE), lysophosphatidylcholines (LPC), and lysophosphatidylethanolamine (LPE). The dynamic changes in the plasma lipidome under the conditions of respiratory viral infections, insulin resistance (IR), and aging indicate a putative role of these different lipids in regulating immune homeostasis in health as well as in acute and chronic inflammation. Moreover, metabolically unhealthy subjects diagnosed with IR show (1) disturbed immune homeostasis and differences in specific lipid-clinical measure associations, (2) altered dynamics for particular complex lipids including TAGs, LPCs PEs, and PCs in response to acute infections, and (3) elevated levels of complex lipids such as TAGs and CEs and accelerated aging, highlighting the importance of context specific interpretation of lipid profiles. Overall, our study exemplifies the power of deep and quantitative lipidomics profiling in conjunction with other omics measures to provide new insights into lipidome dynamics in health and disease.
Institute:Stanford University
Department:Genetics
Laboratory:Snyder
Last Name:Hornburg
First Name:Daniel
Address:Department of Genetics, Stanford University School of Medicine, Stanford, CA, 94305, USA
Email:daniel.dh.hornburg@gmail.com
Phone:650-736-8099

Subject:

Subject ID:SU002802
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id CL4 IR_IS Ethnicity Sex
SA26717402252019_C5_batch05-__320__ZOZOW1T-7042_365Allergy IS C M
SA26717503012019_C5_batch08-__569__ZOZOW1T-7043_520Allergy IS C M
SA26718204032019_C5_batch18-New_1357_NewZTJ7L7Z-09Ant IR A M
SA26718303012019_C5_batch08-__696__ZTJ7L7Z-4011_498Ant IR A M
SA26718403012019_C5_batch08-__99__ZTJ7L7Z-4014_501Ant IR A M
SA26718503062019_C5_batch10-__229__ZX52KVK-4012_631Ant IR C F
SA26718604152019_C5_batch22-__551__ZX52KVK-4013_1413Ant IR C F
SA26718702252019_C5_batch05-__1226__ZX52KVK-4014_318Ant IR C F
SA26718803292019_C5_batch16-__66__ZJXC41N-4013_1020Ant IS B F
SA26718902252019_C5_batch05-__220__ZN3TBJM-4011_319Ant IS C F
SA26719002272019_C5_batch07-__699__ZN3TBJM-4013_414Ant IS C F
SA26719104052019_C5_batch19-__1412__ZN3TBJM-4012_1239Ant IS C F
SA26719204032019_C5_batch18-New_758_NewZOZOW1T-4025Ant IS C M
SA26719304152019_C5_batch22-__1366__ZOZOW1T-4024_1395Ant IS C M
SA26719404222019_C5_batch24-__943__ZOZOW1T-4012_1486Ant IS C M
SA26719503082019_C5_batch11-__466__ZOZOW1T-4021_708Ant IS C M
SA26719604172019_C5_batch23-__1037__ZOZOW1T-4011_1478Ant IS C M
SA26719704102019_C5_batch21-__592__ZOZOW1T-4022_1321Ant IS C M
SA26719802222019_C5_batch05-ZNED4XZ-4013_300Ant Unknown C F
SA26719904152019_C5_batch22-__740__ZNED4XZ-4012_1400Ant Unknown C F
SA26717604052019_C5_batch19-__170__ZX52KVK-4015_1231Ant_L IR C F
SA26717702132019_C5_batch02-ZJXC41N-4015_63Ant_L IS B F
SA26717803112019_C5_batch12-__137__ZN3TBJM-4014_792Ant_L IS C F
SA26717903042019_C5_batch09-__1449__ZN3TBJM-4015_573Ant_L IS C F
SA26718004052019_C5_batch19-__114__ZNED4XZ-4015_1248Ant_L Unknown C F
SA26718102272019_C5_batch07-__151__ZNED4XZ-4014_425Ant_L Unknown C F
SA26720004082019_C5_batch20-__555__ZK112BX-16_1255Cancer IR A F
SA26720103082019_C5_batch11-__325__ZK112BX-15_686Cancer IR A F
SA26720303292019_C5_batch16-__562__ZX52KVK-3013_1064Colonoscopy IR C F
SA26720403292019_C5_batch16-__622__ZX52KVK-3011_1060Colonoscopy IR C F
SA26720503012019_C5_batch08-__379__ZX52KVK-3012_505Colonoscopy IR C F
SA26720602182019_C5_batch03-ZPDABJR-3012_161Colonoscopy IS A M
SA26720702222019_C5_batch05-ZPDABJR-3013_291Colonoscopy IS A M
SA26720802122019_C5_batch01-ZY1ZKJY-10_25Colonoscopy IS C F
SA26720202122019_C5_batch01-ZPDABJR-3014_35Colonoscopy_L IS A M
SA26720903132019_C5_batch13-__1337__ZWCZHHY-E16_850Exercise IR A M
SA26721002272019_C5_batch07-__1142__ZWCZHHY-E17_394Exercise IR A M
SA26721102182019_C5_batch03-ZWCZHHY-E18_Exercise IR A M
SA26721203112019_C5_batch12-__868__ZM7JY3G-E11_801Exercise IR A M
SA26721303252019_C5_batch14-__716__ZWCZHHY-E11_951Exercise IR A M
SA26721403272019_C5_batch15-New_107_NewZVM4N7A-01-E14Exercise IR C F
SA26721503252019_C5_batch14-New_111_NewZVM4N7A-01-E17Exercise IR C F
SA26721604082019_C5_batch20-New_112_NewZVM4N7A-01-E18Exercise IR C F
SA26721703292019_C5_batch16-New_110_NewZVM4N7A-01-E16Exercise IR C F
SA26721804102019_C5_batch21-New_109_NewZVM4N7A-01-E15Exercise IR C F
SA26721903272019_C5_batch15-New_108_NewZVM4N7A-01-E13Exercise IR C F
SA26722004052019_C5_batch19-New_106_NewZVM4N7A-01-E12Exercise IR C F
SA26722104032019_C5_batch18-New_104_NewZVM4N7A-01-E11Exercise IR C F
SA26722204052019_C5_batch19-New_115_NewZY7IW45-02-E12Exercise IR C F
SA26722303062019_C5_batch10-__886__ZY7IW45-E11_623Exercise IR C F
SA26722404052019_C5_batch19-New_114_NewZY7IW45-02-E13Exercise IR C F
SA26722503252019_C5_batch14-New_113_NewZY7IW45-02-E11Exercise IR C F
SA26722604082019_C5_batch20-New_116_NewZY7IW45-02-E14Exercise IR C F
SA26722704102019_C5_batch21-New_118_NewZY7IW45-02-E17Exercise IR C F
SA26722803292019_C5_batch16-New_119_NewZY7IW45-02-E16Exercise IR C F
SA26722904082019_C5_batch20-New_38_NewZL63I8R-E12Exercise IR C M
SA26723004012019_C5_batch17-New_97_NewZPF36E2-8021Exercise IR C M
SA26723104012019_C5_batch17-New_36_NewZL63I8R-E13Exercise IR C M
SA26723204152019_C5_batch22-New_42_NewZL63I8R-E15Exercise IR C M
SA26723303292019_C5_batch16-New_41_NewZL63I8R-E17Exercise IR C M
SA26723404082019_C5_batch20-New_43_NewZL63I8R-E14Exercise IR C M
SA26723504012019_C5_batch17-New_91_NewZPF36E2-01-E14Exercise IR C M
SA26723604102019_C5_batch21-New_89_NewZPF36E2-01-E13Exercise IR C M
SA26723703292019_C5_batch16-New_88_NewZPF36E2-01-E12Exercise IR C M
SA26723803292019_C5_batch16-New_40_NewZL63I8R-E18Exercise IR C M
SA26723904102019_C5_batch21-New_90_NewZPF36E2-01-E15Exercise IR C M
SA26724004052019_C5_batch19-New_92_NewZPF36E2-01-E16Exercise IR C M
SA26724103292019_C5_batch16-New_93_NewZPF36E2-01-E17Exercise IR C M
SA26724203252019_C5_batch14-New_39_NewZL63I8R-E16Exercise IR C M
SA26724302252019_C5_batch05-__276__ZVBQY1N-E11_353Exercise IR C M
SA26724404152019_C5_batch22-New_66_NewZLZQMEV-02-E18Exercise IR H F
SA26724504032019_C5_batch18-New_27_NewZRB0F6P-02-E12Exercise IR H F
SA26724604172019_C5_batch23-New_67_NewZLZQMEV-02-E17Exercise IR H F
SA26724704052019_C5_batch19-New_70_NewZLZQMEV-02-E15Exercise IR H F
SA26724804102019_C5_batch21-New_69_NewZLZQMEV-02-E14Exercise IR H F
SA26724904152019_C5_batch22-New_29_NewZRB0F6P-02-E13Exercise IR H F
SA26725003252019_C5_batch14-New_71_NewZLZQMEV-02-E13Exercise IR H F
SA26725104012019_C5_batch17-New_32_NewZRB0F6P-02-E17Exercise IR H F
SA26725203292019_C5_batch16-New_31_NewZRB0F6P-02-E16Exercise IR H F
SA26725303292019_C5_batch16-New_30_NewZRB0F6P-02-E15Exercise IR H F
SA26725404102019_C5_batch21-New_28_NewZRB0F6P-02-E14Exercise IR H F
SA26725504172019_C5_batch23-New_33_NewZRB0F6P-02-E18Exercise IR H F
SA26725604012019_C5_batch17-New_68_NewZLZQMEV-02-E16Exercise IR H F
SA26725704032019_C5_batch18-New_64_NewZLZQMEV-02-E11Exercise IR H F
SA26725804102019_C5_batch21-New_65_NewZLZQMEV-02-E12Exercise IR H F
SA26725904102019_C5_batch21-New_83_NewZMBVNFM-02-E17Exercise IS A M
SA26726004032019_C5_batch18-New_77_NewZMBVNFM-02-E14Exercise IS A M
SA26726104152019_C5_batch22-New_80_NewZMBVNFM-02-E15Exercise IS A M
SA26726204172019_C5_batch23-New_84_NewZMBVNFM-02-E18Exercise IS A M
SA26726303292019_C5_batch16-New_82_NewZMBVNFM-02-E11Exercise IS A M
SA26726404082019_C5_batch20-New_81_NewZMBVNFM-02-E16Exercise IS A M
SA26726503292019_C5_batch16-New_79_NewZMBVNFM-02-E12Exercise IS A M
SA26726603252019_C5_batch14-New_78_NewZMBVNFM-02-E13Exercise IS A M
SA26726704172019_C5_batch23-New_58_NewZPEL6L3-02-E16Exercise IS C F
SA26726804082019_C5_batch20-New_55_NewZPEL6L3-02-E12Exercise IS C F
SA26726903272019_C5_batch15-New_60_NewZPEL6L3-02-E18Exercise IS C F
SA26727004152019_C5_batch22-New_57_NewZPEL6L3-02-E15Exercise IS C F
SA26727104032019_C5_batch18-New_612_NewZQFL1P3-03Exercise IS C F
SA26727204032019_C5_batch18-New_571_NewZS2DMX7-02Exercise IS C F
SA26727303252019_C5_batch14-New_59_NewZPEL6L3-02-E17Exercise IS C F
Showing page 1 of 17     Results:    1  2  3  4  5  Next  Last     Showing results 1 to 100 of 1643

Collection:

Collection ID:CO002795
Collection Summary:Standard blood plasma collection
Sample Type:Blood (plasma)

Treatment:

Treatment ID:TR002811
Treatment Summary:Not applicable

Sample Preparation:

Sampleprep ID:SP002808
Sampleprep Summary:Plasma samples were prepared and analyzed in a randomized order. Plasma was thawed on ice and lipids were extracted using a biphasic separation (ice-cold methanol, methyl tert-butyl ether (MTBE) and water). 260 µl of methanol and 40 ul spike-in standard (cat# 5040156, Sciex, Redwood City, CA, USA) were added to 40 µl of plasma and vortexed for 20 s. Lipids were extracted by adding 1,000 µl of MTBE and incubated under agitation for 30 min at 4°C. Phase separation was induced by adding 250 µl of ice-cold water, followed by vortexing for 1 min and centrifugation at 14,000 g for 15 min at 4°C. The upper phase containing the lipids was collected, dried down under nitrogen, stored at -20°C in 200 ul MeOH. The day of the mass spectrometry acquisition, lipids were dried down under nitrogen and reconstituted with 300 µl of 10 mM ammonium acetate in 9:1 methanol:toluene.
Processing Storage Conditions:-80℃
Extraction Method:MTBE
Extract Storage:-20℃

Combined analysis:

Analysis ID AN003790
Analysis type MS
Chromatography type None (Direct infusion)
Chromatography system none
Column none
MS Type ESI
MS instrument type QTRAP
MS instrument name ABI Sciex 5500 QTrap
Ion Mode UNSPECIFIED
Units nmol/ml

Chromatography:

Chromatography ID:CH003279
Instrument Name:none
Column Name:none
Chromatography Type:None (Direct infusion)

MS:

MS ID:MS004122
Analysis ID:AN003790
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:QTRAP
MS Type:ESI
MS Comments:Lipidyzer raw data exported with MSConvert
Ion Mode:UNSPECIFIED
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