Summary of Study ST002171
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001381. The data can be accessed directly via it's Project DOI: 10.21228/M86T35 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002171 |
| Study Title | Serum metabolome of specific-pathogen-free mice fed with five distinct rodent diets with varying fiber content and source. |
| Study Summary | In an effort to discern the effects of diverse fibers on host immunity, we employed five distinct rodent diets with varying fiber content and source in specific-pathogen-free. |
| Institute | Luxembourg Institute of Health |
| Department | Department of Infection and Immunity |
| Last Name | Desai |
| First Name | Mahesh S |
| Address | 4354 Esch-sur-Alzette, Luxembourg |
| mahesh.desai@lih.lu | |
| Phone | +352 26970-389 |
| Submit Date | 2022-05-19 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-05-18 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001381 |
| Project DOI: | doi: 10.21228/M86T35 |
| Project Title: | Cecal and serum metabolome of specific-pathogen-free, gnotobiotic (containing a 14-member synthetic human gut microbiota), and germ-free mice fed with five distinct rodent diets with varying fiber content and source. |
| Project Summary: | In an effort to discern the effects of diverse fibers on host immunity, we employed five distinct rodent diets with varying fiber content and source in specific-pathogen-free, gnotobiotic (containing a 14-member synthetic human gut microbiota), and germ-free mice. |
| Institute: | Luxembourg Institute of Health |
| Department: | Department of Infection and Immunity |
| Last Name: | Desai |
| First Name: | Mahesh S |
| Address: | 4354 Esch-sur-Alzette, Luxembourg |
| Email: | mahesh.desai@lih.lu |
| Phone: | +352 26970-389 |
Subject:
| Subject ID: | SU002257 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male and female |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Organ | Treatment | Diet |
|---|---|---|---|---|
| SA208888 | Serum_566_FF1 | Serum | SPF | Fiber-free |
| SA208889 | Serum_567_FF2 | Serum | SPF | Fiber-free |
| SA208890 | Serum_568_FF3 | Serum | SPF | Fiber-free |
| SA208891 | Serum_569_FF4 | Serum | SPF | Fiber-free |
| SA208892 | Serum_561_FR2 | Serum | SPF | Fiber-rich |
| SA208893 | Serum_563_FR4 | Serum | SPF | Fiber-rich |
| SA208894 | Serum_560_FR1 | Serum | SPF | Fiber-rich |
| SA208895 | Serum_562_FR3 | Serum | SPF | Fiber-rich |
| SA208896 | Serum_FS_2 | Serum | SPF | Fiber-supplemented |
| SA208897 | Serum_FS_7 | Serum | SPF | Fiber-supplemented |
| SA208898 | Serum_FS_5 | Serum | SPF | Fiber-supplemented |
| SA208899 | Serum_FS_1 | Serum | SPF | Fiber-supplemented |
| SA208900 | Serum_In6 | Serum | SPF | Inulin-supplemented |
| SA208901 | Serum_In4 | Serum | SPF | Inulin-supplemented |
| SA208902 | Serum_In3 | Serum | SPF | Inulin-supplemented |
| SA208903 | Serum_In1 | Serum | SPF | Inulin-supplemented |
| SA208904 | Serum_LD_5 | Serum | SPF | LabDiet |
| SA208905 | Serum_LD_6 | Serum | SPF | LabDiet |
| SA208906 | Serum_LD_4 | Serum | SPF | LabDiet |
| SA208907 | Serum_LD_8 | Serum | SPF | LabDiet |
| Showing results 1 to 20 of 20 |
Collection:
| Collection ID: | CO002250 |
| Collection Summary: | Serum metabolome of specific-pathogen-free mice fed with five distinct rodent diets with varying fiber content and source. |
| Sample Type: | Serum |
Treatment:
| Treatment ID: | TR002269 |
| Treatment Summary: | Experiments were performed with 6-week old female BALB/c mice (Charles River Laboratories, France), housed in the specific-pathogen-free (SPF) facility of the Luxembourg Institute of Health. Mice were fed on their respective diets for 40 days and then sacrificed by cervical dislocation. |
Sample Preparation:
| Sampleprep ID: | SP002263 |
| Sampleprep Summary: | Serum metabolites were extracted using the methanol:chloroform:water extraction protocol. |
Combined analysis:
| Analysis ID | AN003557 | AN003558 |
|---|---|---|
| Chromatography ID | CH002628 | CH002629 |
| MS ID | MS003314 | MS003315 |
| Analysis type | MS | MS |
| Chromatography type | CE | CE |
| Chromatography system | Agilent CE | Agilent CE |
| Column | Fused-silica,50um(cation) | COSMO(+),50um(anion |
| MS Type | ESI | ESI |
| MS instrument type | TOF | TOF |
| MS instrument name | Agilent CE-TOFMS | Agilent CE-TOFMS |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | μM | μM |
Chromatography:
| Chromatography ID: | CH002628 |
| Instrument Name: | Agilent CE |
| Column Name: | Fused-silica,50um(cation) |
| Chromatography Type: | CE |
| Chromatography ID: | CH002629 |
| Instrument Name: | Agilent CE |
| Column Name: | COSMO(+),50um(anion |
| Chromatography Type: | CE |
MS:
| MS ID: | MS003314 |
| Analysis ID: | AN003557 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | TOF |
| MS Type: | ESI |
| MS Comments: | In-house software (MasterHands) was used for peak area integration and annotation. |
| Ion Mode: | POSITIVE |
| MS ID: | MS003315 |
| Analysis ID: | AN003558 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | TOF |
| MS Type: | ESI |
| MS Comments: | In-house software (MasterHands) was used for peak area integration and annotation. |
| Ion Mode: | NEGATIVE |
