Summary of Study ST002300

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001473. The data can be accessed directly via it's Project DOI: 10.21228/M8B40C This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002300
Study TitleCharacterizing the intrauterine environment via untargeted metabolomics profiling of maternal blood collected during pregnancy (Healthy Start Cohort)-Part 2
Study TypeProspective cohort study
Study SummaryHealthy Start is a prospective, pre-birth cohort study that recruited pregnant participants from outpatient prenatal clinics at the University of Colorado Hospital between 2009 and 2014. Eligible participants were 16 years or older with singleton pregnancies, no history of stillbirth or extremely preterm birth (<25 weeks of gestation) and no serious medical conditions, and had not yet completed 24 weeks of gestation at the time of enrollment. Mothers completed two study visits during pregnancy (median gestational ages 17 and 27 weeks). Mother-child pairs were assessed at birth for neonatal outcomes, and are currently still being followed through 8-10 years postpartum (NB: outcomes for this proposal are at birth). For the present project, we will use data from 1,297 mother-child pairs with adequate maternal plasma at 17 and 27 gestational weeks for untargeted metabolomics profiling. Please contact Wei Perng at wei.perng@cuanschutz.edu for questions related to the subject characteristics and outcomes. This research was supported by the Environmental influences on Child Health Outcomes (ECHO) program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Healthy Start is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375 ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382 North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539
Institute
University of Colorado Anschutz Medical Campus
DepartmentEpidemiology; Lifecourse Epidemiology of Adiposity and Diabetes (LEAD) Center
Last NamePerng
First NameWei
Address1890 North Revere Court, Campus Box F426, Rm 1014, Aurora, CO 80010
EmailWei.Perng@CUAnschutz.edu
Phone734-717-0982
Submit Date2022-09-12
Total Subjects2202
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Chear StudyYes
Analysis Type DetailLC-MS
Release Date2023-10-02
Release Version1
Wei Perng Wei Perng
https://dx.doi.org/10.21228/M8B40C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001473
Project DOI:doi: 10.21228/M8B40C
Project Title:Characterizing the intrauterine environment via untargeted metabolomics profiling of maternal blood collected during pregnancy (Healthy Start Cohort)
Project Type:C18 Reversed-Phase Broad Spectrum Metabolomics
Project Summary:This project is funded by the NIH Environmental influences on Child Health Outcomes (ECHO) program, and uses data from five cohorts: Healthy Start, Project Viva, Atlanta ECHO, Paneth Cohort, and PETALS. This project will generate new untargeted metabolomics profiling data at two time-points in pregnancy for all women in order to identify trimester-specific metabolomics signatures and longitudinal metabolomics trajectories associated with offspring birth size; identify the contribution of maternal sociodemographic and perinatal characteristics to the metabolomics signatures.
Institute:NC HHEAR Hub
Department:Untargeted Analysis
Laboratory:Sumner Lab
Last Name:Li
First Name:Yuanyuan
Address:500 Laureate Way, Kannapolis, NC 28081
Email:yuanyli4@unc.edu
Phone:9843770693
Funding Source:This research was supported by the Environmental influences on Child Health Outcomes (ECHO) program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Healthy Start is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375; ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382; North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857; Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539

Subject:

Subject ID:SU002386
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Female

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample Type
SA223686PZDa_HHEAR_149HHEAR Pool
SA223687PZDa_HHEAR_148HHEAR Pool
SA223688PZDa_HHEAR_150HHEAR Pool
SA223689PZDa_HHEAR_151HHEAR Pool
SA223690PZDa_HHEAR_152HHEAR Pool
SA223691PZDa_HHEAR_147HHEAR Pool
SA223692PZDa_HHEAR_145HHEAR Pool
SA223693PZDa_HHEAR_142HHEAR Pool
SA223694PZDa_HHEAR_141HHEAR Pool
SA223695PZDa_HHEAR_143HHEAR Pool
SA223696PZDa_HHEAR_144HHEAR Pool
SA223697PZDa_HHEAR_153HHEAR Pool
SA223698PZDa_HHEAR_146HHEAR Pool
SA223699PZDa_HHEAR_155HHEAR Pool
SA223700PZDa_HHEAR_163HHEAR Pool
SA223701PZDa_HHEAR_162HHEAR Pool
SA223702PZDa_HHEAR_164HHEAR Pool
SA223703PZDa_HHEAR_165HHEAR Pool
SA223704PZDa_HHEAR_166HHEAR Pool
SA223705PZDa_HHEAR_161HHEAR Pool
SA223706PZDa_HHEAR_160HHEAR Pool
SA223707PZDa_HHEAR_156HHEAR Pool
SA223708PZDa_HHEAR_140HHEAR Pool
SA223709PZDa_HHEAR_157HHEAR Pool
SA223710PZDa_HHEAR_158HHEAR Pool
SA223711PZDa_HHEAR_159HHEAR Pool
SA223712PZDa_HHEAR_154HHEAR Pool
SA223713PZDa_HHEAR_138HHEAR Pool
SA223714PZDa_HHEAR_121HHEAR Pool
SA223715PZDa_HHEAR_120HHEAR Pool
SA223716PZDa_HHEAR_122HHEAR Pool
SA223717PZDa_HHEAR_123HHEAR Pool
SA223718PZDa_HHEAR_124HHEAR Pool
SA223719PZDa_HHEAR_119HHEAR Pool
SA223720PZDa_HHEAR_118HHEAR Pool
SA223721PZDa_HHEAR_114HHEAR Pool
SA223722PZDa_HHEAR_113HHEAR Pool
SA223723PZDa_HHEAR_115HHEAR Pool
SA223724PZDa_HHEAR_116HHEAR Pool
SA223725PZDa_HHEAR_117HHEAR Pool
SA223726PZDa_HHEAR_125HHEAR Pool
SA223727PZDa_HHEAR_126HHEAR Pool
SA223728PZDa_HHEAR_135HHEAR Pool
SA223729PZDa_HHEAR_134HHEAR Pool
SA223730PZDa_HHEAR_136HHEAR Pool
SA223731PZDa_HHEAR_137HHEAR Pool
SA223732PZDa_HHEAR_167HHEAR Pool
SA223733PZDa_HHEAR_133HHEAR Pool
SA223734PZDa_HHEAR_132HHEAR Pool
SA223735PZDa_HHEAR_128HHEAR Pool
SA223736PZDa_HHEAR_127HHEAR Pool
SA223737PZDa_HHEAR_129HHEAR Pool
SA223738PZDa_HHEAR_130HHEAR Pool
SA223739PZDa_HHEAR_131HHEAR Pool
SA223740PZDa_HHEAR_139HHEAR Pool
SA223741PZDa_HHEAR_168HHEAR Pool
SA223742PZDa_HHEAR_204HHEAR Pool
SA223743PZDa_HHEAR_203HHEAR Pool
SA223744PZDa_HHEAR_205HHEAR Pool
SA223745PZDa_HHEAR_206HHEAR Pool
SA223746PZDa_HHEAR_207HHEAR Pool
SA223747PZDa_HHEAR_202HHEAR Pool
SA223748PZDa_HHEAR_201HHEAR Pool
SA223749PZDa_HHEAR_197HHEAR Pool
SA223750PZDa_HHEAR_196HHEAR Pool
SA223751PZDa_HHEAR_198HHEAR Pool
SA223752PZDa_HHEAR_199HHEAR Pool
SA223753PZDa_HHEAR_200HHEAR Pool
SA223754PZDa_HHEAR_209HHEAR Pool
SA223755PZDa_HHEAR_210HHEAR Pool
SA223756PZDa_HHEAR_219HHEAR Pool
SA223757PZDa_HHEAR_218HHEAR Pool
SA223758PZDa_HHEAR_220HHEAR Pool
SA223759PZDa_HHEAR_221HHEAR Pool
SA223760PZDa_HHEAR_1HHEAR Pool
SA223761PZDa_HHEAR_217HHEAR Pool
SA223762PZDa_HHEAR_216HHEAR Pool
SA223763PZDa_HHEAR_212HHEAR Pool
SA223764PZDa_HHEAR_211HHEAR Pool
SA223765PZDa_HHEAR_213HHEAR Pool
SA223766PZDa_HHEAR_214HHEAR Pool
SA223767PZDa_HHEAR_215HHEAR Pool
SA223768PZDa_HHEAR_195HHEAR Pool
SA223769PZDa_HHEAR_194HHEAR Pool
SA223770PZDa_HHEAR_177HHEAR Pool
SA223771PZDa_HHEAR_176HHEAR Pool
SA223772PZDa_HHEAR_178HHEAR Pool
SA223773PZDa_HHEAR_179HHEAR Pool
SA223774PZDa_HHEAR_180HHEAR Pool
SA223775PZDa_HHEAR_175HHEAR Pool
SA223776PZDa_HHEAR_174HHEAR Pool
SA223777PZDa_HHEAR_170HHEAR Pool
SA223778PZDa_HHEAR_169HHEAR Pool
SA223779PZDa_HHEAR_171HHEAR Pool
SA223780PZDa_HHEAR_172HHEAR Pool
SA223781PZDa_HHEAR_173HHEAR Pool
SA223782PZDa_HHEAR_181HHEAR Pool
SA223783PZDa_HHEAR_182HHEAR Pool
SA223784PZDa_HHEAR_190HHEAR Pool
SA223785PZDa_HHEAR_189HHEAR Pool
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Collection:

Collection ID:CO002379
Collection Summary:Healthy Start is a prospective, pre-birth cohort study that recruited pregnant participants from outpatient prenatal clinics at the University of Colorado Hospital between 2009 and 2014. Eligible participants were 16 years or older with singleton pregnancies, no history of stillbirth or extremely preterm birth (<25 weeks of gestation) and no serious medical conditions, and had not yet completed 24 weeks of gestation at the time of enrollment. Mothers completed two study visits during pregnancy (median gestational ages 17 and 27 weeks). Mother-child pairs were assessed at birth for neonatal outcomes, and are currently still being followed through 8-10 years postpartum (NB: outcomes for this proposal are at birth). For the present project, we will use data from 1,297 mother-child pairs with adequate maternal plasma at 17 and 27 gestational weeks for untargeted metabolomics profiling.
Sample Type:Blood (serum)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002398
Treatment Summary:N/A

Sample Preparation:

Sampleprep ID:SP002392
Sampleprep Summary:The LEAD Center of the University of Colorado Denver Anschutz Medical Campus aliquoted the study samples (50 µL) and made the total study pool sample according to the statement of work provided by UNC. These samples were shipped to the NC HHEAR Hub on dry ice. Before sample preparation, the NC HHEAR Hub thawed and mixed the total study pool samples and distributed them with 50 µL per aliquot, which were used as QC study pools throughout the whole study. HHEAR reference plasma (50 µL each) and NIST serum (909C) reference material (50 µL each) were provided by the NC HHEAR Hub. The LC-MS grade water (50 µL) was used as blank. Sample preparation was conducted by batch and all samples were thawed at 4°C overnight before the preparation. Samples, including study samples, study pool samples, HHEAR reference plasma, NIST reference serum, and blanks were mixed with 400 µL methanol containing 500 ng/mL tryptophan-d5 as internal standard and vortexed by a multiple tube vortex mixer for 2 min at 5000 rpm in room temperature. All samples were centrifuged at 16,000 rcf for 10 min at 4°C. The supernatant (350 µL) was transferred into a pre-labeled 2.0 mL Lo-bind Eppendorf tube, dried by a SpeedVac overnight, and stored at -80° C. For immediate analysis, 100 µL of water-methanol solution (95:5, v/v) was used to reconstitute the dried extracts. Samples were thoroughly mixed on a multiple tube vortex mixer for 10 min at 5000 rpm at room temperature and then centrifuged at 4°C for 10 min at 16,000 rcf. The supernatant was transferred to pre-labeled autosampler vials for data acquisition by LC-MS.
Processing Storage Conditions:On ice
Extract Storage:Described in summary

Combined analysis:

Analysis ID AN003756
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Scientific™ Vanquish™ UPHPLC
Column Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF-X Orbitrap
Ion Mode POSITIVE
Units m/z ratio

Chromatography:

Chromatography ID:CH002779
Chromatography Summary:Reverse phase
Instrument Name:Thermo Scientific™ Vanquish™ UPHPLC
Column Name:Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um)
Column Pressure:6000-10000
Column Temperature:50
Flow Gradient:Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 6. 22.00 0.4 99.0 1.0 5
Flow Rate:0.4 ml/min
Injection Temperature:8
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Randomization Order:Randomized
Chromatography Type:Reversed phase

MS:

MS ID:MS003499
Analysis ID:AN003756
Instrument Name:Thermo Q Exactive HF-X Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Instrument: Thermo Q Exactive HFx Software: Xcalibur 4.1.31.9 for data acquisition; Progenesis QI 2.4 for data preprocessing
Ion Mode:POSITIVE
Capillary Temperature:320 °C
Capillary Voltage:3.5 KV
Collision Energy:20-45, ramp
Collision Gas:N2
Dry Gas Flow:55
Dry Gas Temp:400°C
Fragmentation Method:CID
Desolvation Gas Flow:55
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