Summary of Study ST002336

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001701. The data can be accessed directly via it's Project DOI: 10.21228/M8TT5H This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002336
Study TitleCharacterizing the intrauterine environment via untargeted metabolomics profiling of maternal blood collected during pregnancy (Project Viva)
Study TypeProspective cohort study
Study SummaryProject Viva: Pregnant women were enrolled in Project Viva between 1999 and 2002 at their first prenatal visit at one of 8 obstetric clinics of Atrius Harvard Vanguard Medical Associates, a multispecialty group practice in eastern Massachusetts. Eligible mothers were fluent in English, had singleton gestations, were <22 weeks gestation, and had no plans to move away from the study area. Research staff performed in-person study visits with participating mothers in the first (median gestational age 9.9 weeks) and second (median gestational age 28.1 weeks) trimesters of pregnancy, and with mothers and children during the first few days after delivery, during infancy (median age 6.3 months), in early childhood (median age 3.3 years), mid-childhood (median age 7.7 years), and adolescence (median age13 years). In this analysis, we will use data from 188 mother-child pairs in Project Viva with available information on prenatal PFAS concentrations, available umbilical cord serum samples at delivery, and outcomes of interest. Please contact MollyAn Killingbeck at mollyan.killingbeck@point32health.org for questions related to the subject characteristics and outcomes. This research was supported by the Environmental influences on Child Health Outcomes (ECHO) program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Project Viva is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375 ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382 North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539
Institute
Harvard Pilgrim Health Care Institute
DepartmentPopulation Medicine
LaboratoryChanning
Last NameOken
First NameEmily
Address401 Park Drive, Ste 401, Boston, MA 02215
Emailemily_oken@harvardpilgrim.org
Phone617-867-4835
Submit Date2022-10-21
Total Subjects1184
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Chear StudyYes
Analysis Type DetailLC-MS
Release Date2023-11-03
Release Version1
Emily Oken Emily Oken
https://dx.doi.org/10.21228/M8TT5H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001701
Project DOI:doi: 10.21228/M8TT5H
Project Title:Characterizing the intrauterine environment via untargeted metabolomics profiling of maternal blood collected during pregnancy (Project Viva)
Project Type:C18 Reversed-Phase Broad Spectrum Metabolomics
Project Summary:This project is funded by the NIH Environmental influences on Child Health Outcomes (ECHO) program, and uses data from five cohorts: Healthy Start, Project Viva, Atlanta ECHO, Paneth Cohort, and PETALS. This project will generate new untargeted metabolomics profiling data at two time-points in pregnancy for all women in order to identify trimester-specific metabolomics signatures and longitudinal metabolomics trajectories associated with offspring birth size; identify the contribution of maternal sociodemographic and perinatal characteristics to the metabolomics signatures.
Institute:NC HHEAR Hub
Department:Untargeted Analysis
Laboratory:Sumner Lab
Last Name:Li
First Name:Yuanyuan
Address:500 Laureate Way, Kannapolis, NC 28081
Email:yuanyli4@unc.edu
Phone:9843770693
Funding Source:This research was supported by the Environmental influences on Child Health Outcomes (ECHO) program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Project Viva is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375; ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382; North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857; Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539

Subject:

Subject ID:SU002425
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Male and female

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample Type
SA232419PZOk_HHEAR_81HHEAR Pool
SA232420PZOk_HHEAR_82HHEAR Pool
SA232421PZOk_HHEAR_80HHEAR Pool
SA232422PZOk_HHEAR_78HHEAR Pool
SA232423PZOk_HHEAR_77HHEAR Pool
SA232424PZOk_HHEAR_83HHEAR Pool
SA232425PZOk_HHEAR_79HHEAR Pool
SA232426PZOk_HHEAR_84HHEAR Pool
SA232427PZOk_HHEAR_88HHEAR Pool
SA232428PZOk_HHEAR_89HHEAR Pool
SA232429PZOk_HHEAR_87HHEAR Pool
SA232430PZOk_HHEAR_86HHEAR Pool
SA232431PZOk_HHEAR_85HHEAR Pool
SA232432PZOk_HHEAR_76HHEAR Pool
SA232433PZOk_HHEAR_74HHEAR Pool
SA232434PZOk_HHEAR_66HHEAR Pool
SA232435PZOk_HHEAR_67HHEAR Pool
SA232436PZOk_HHEAR_65HHEAR Pool
SA232437PZOk_HHEAR_64HHEAR Pool
SA232438PZOk_HHEAR_62HHEAR Pool
SA232439PZOk_HHEAR_63HHEAR Pool
SA232440PZOk_HHEAR_68HHEAR Pool
SA232441PZOk_HHEAR_69HHEAR Pool
SA232442PZOk_HHEAR_73HHEAR Pool
SA232443PZOk_HHEAR_90HHEAR Pool
SA232444PZOk_HHEAR_72HHEAR Pool
SA232445PZOk_HHEAR_71HHEAR Pool
SA232446PZOk_HHEAR_70HHEAR Pool
SA232447PZOk_HHEAR_75HHEAR Pool
SA232448PZOk_HHEAR_92HHEAR Pool
SA232449PZOk_HHEAR_111HHEAR Pool
SA232450PZOk_HHEAR_113HHEAR Pool
SA232451PZOk_HHEAR_110HHEAR Pool
SA232452PZOk_HHEAR_109HHEAR Pool
SA232453PZOk_HHEAR_107HHEAR Pool
SA232454PZOk_HHEAR_108HHEAR Pool
SA232455PZOk_HHEAR_114HHEAR Pool
SA232456PZOk_HHEAR_115HHEAR Pool
SA232457PZOk_HHEAR_119HHEAR Pool
SA232458PZOk_HHEAR_1HHEAR Pool
SA232459PZOk_HHEAR_118HHEAR Pool
SA232460PZOk_HHEAR_117HHEAR Pool
SA232461PZOk_HHEAR_116HHEAR Pool
SA232462PZOk_HHEAR_106HHEAR Pool
SA232463PZOk_HHEAR_105HHEAR Pool
SA232464PZOk_HHEAR_96HHEAR Pool
SA232465PZOk_HHEAR_97HHEAR Pool
SA232466PZOk_HHEAR_95HHEAR Pool
SA232467PZOk_HHEAR_94HHEAR Pool
SA232468PZOk_HHEAR_61HHEAR Pool
SA232469PZOk_HHEAR_93HHEAR Pool
SA232470PZOk_HHEAR_98HHEAR Pool
SA232471PZOk_HHEAR_99HHEAR Pool
SA232472PZOk_HHEAR_103HHEAR Pool
SA232473PZOk_HHEAR_104HHEAR Pool
SA232474PZOk_HHEAR_102HHEAR Pool
SA232475PZOk_HHEAR_101HHEAR Pool
SA232476PZOk_HHEAR_100HHEAR Pool
SA232477PZOk_HHEAR_91HHEAR Pool
SA232478PZOk_HHEAR_112HHEAR Pool
SA232479PZOk_HHEAR_60HHEAR Pool
SA232480PZOk_HHEAR_22HHEAR Pool
SA232481PZOk_HHEAR_20HHEAR Pool
SA232482PZOk_HHEAR_19HHEAR Pool
SA232483PZOk_HHEAR_17HHEAR Pool
SA232484PZOk_HHEAR_18HHEAR Pool
SA232485PZOk_HHEAR_23HHEAR Pool
SA232486PZOk_HHEAR_24HHEAR Pool
SA232487PZOk_HHEAR_28HHEAR Pool
SA232488PZOk_HHEAR_29HHEAR Pool
SA232489PZOk_HHEAR_27HHEAR Pool
SA232490PZOk_HHEAR_26HHEAR Pool
SA232491PZOk_HHEAR_25HHEAR Pool
SA232492PZOk_HHEAR_16HHEAR Pool
SA232493PZOk_HHEAR_15HHEAR Pool
SA232494PZOk_HHEAR_7HHEAR Pool
SA232495PZOk_HHEAR_8HHEAR Pool
SA232496PZOk_HHEAR_6HHEAR Pool
SA232497PZOk_HHEAR_5HHEAR Pool
SA232498PZOk_HHEAR_3HHEAR Pool
SA232499PZOk_HHEAR_4HHEAR Pool
SA232500PZOk_HHEAR_9HHEAR Pool
SA232501PZOk_HHEAR_10HHEAR Pool
SA232502PZOk_HHEAR_13HHEAR Pool
SA232503PZOk_HHEAR_14HHEAR Pool
SA232504PZOk_HHEAR_12HHEAR Pool
SA232505PZOk_HHEAR_11HHEAR Pool
SA232506PZOk_HHEAR_2HHEAR Pool
SA232507PZOk_HHEAR_30HHEAR Pool
SA232508PZOk_HHEAR_21HHEAR Pool
SA232509PZOk_HHEAR_51HHEAR Pool
SA232510PZOk_HHEAR_52HHEAR Pool
SA232511PZOk_HHEAR_50HHEAR Pool
SA232512PZOk_HHEAR_49HHEAR Pool
SA232513PZOk_HHEAR_47HHEAR Pool
SA232514PZOk_HHEAR_48HHEAR Pool
SA232515PZOk_HHEAR_53HHEAR Pool
SA232516PZOk_HHEAR_54HHEAR Pool
SA232517PZOk_HHEAR_58HHEAR Pool
SA232518PZOk_HHEAR_59HHEAR Pool
Showing page 1 of 15     Results:    1  2  3  4  5  Next  Last     Showing results 1 to 100 of 1467

Collection:

Collection ID:CO002418
Collection Summary:Project Viva: Pregnant women were enrolled in Project Viva between 1999 and 2002 at their first prenatal visit at one of 8 obstetric clinics of Atrius Harvard Vanguard Medical Associates, a multispecialty group practice in eastern Massachusetts. Eligible mothers were fluent in English, had singleton gestations, were <22 weeks gestation, and had no plans to move away from the study area. Research staff performed in-person study visits with participating mothers in the first (median gestational age 9.9 weeks) and second (median gestational age 28.1 weeks) trimesters of pregnancy, and with mothers and children during the first few days after delivery, during infancy (median age 6.3 months), in early childhood (median age 3.3 years), mid-childhood (median age 7.7 years), and adolescence (median age13 years). In this analysis, we will use data from 188 mother-child pairs in Project Viva with available information on prenatal PFAS concentrations, available umbilical cord serum samples at delivery, and outcomes of interest. Please contact MollyAn Killingbeck at mollyan.killingbeck@point32health.org for questions related to the subject characteristics and outcomes.
Sample Type:Blood (plasma)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002437
Treatment Summary:N/A

Sample Preparation:

Sampleprep ID:SP002431
Sampleprep Summary:Brigham and Women’s Hospital Channing aliquoted the study samples (50 µL) and made the total study pool sample according to the statement of work provided by UNC. These samples were shipped to the NC HHEAR Hub on dry ice. Before sample preparation, the NC HHEAR Hub thawed and mixed the total study pool samples and distributed it with 50 µL per aliquot, which were used as QC study pools throughout the whole study. HHEAR reference plasma (50 µL each) and NIST plasma (1950) reference material (50 µL each) were provided by the NC HHEAR Hub. The LC-MS grade water (50 µL) was used as blank. Sample preparation was conducted by batch and all samples were thawed at 4°C overnight before the preparation. Samples, including study samples, study pool samples, HHEAR reference plasma, NIST reference plasma, and blanks were mixed with 400 µL methanol containing 500 ng/mL tryptophan-d5 as internal standard and vortexed by a multiple tube vortex mixer for 2 min at 5000 rpm at room temperature. All samples were centrifuged at 16,000 rcf for 10 min at 4°C. The supernatant (350 µL) was transferred into a pre-labeled 2.0 mL Lo-bind Eppendorf tube, dried by a SpeedVac overnight, and stored at -80° C. For immediate analysis, 100 µL of water-methanol solution (95:5, v/v) was used to reconstitute the dried extracts. Samples were thoroughly mixed on a multiple tube vortex mixer for 10 min at 5000 rpm at room temperature and then centrifuged at 4°C for 10 min at 16,000 rcf. The supernatant was transferred to pre-labeled autosampler vials for data acquisition by LC-MS.
Processing Storage Conditions:On ice
Extract Storage:Described in summary

Combined analysis:

Analysis ID AN003818
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Scientific™ Vanquish™ UPHPLC
Column Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF-X Orbitrap
Ion Mode POSITIVE
Units Normalized intensity

Chromatography:

Chromatography ID:CH002825
Chromatography Summary:Reverse phase
Instrument Name:Thermo Scientific™ Vanquish™ UPHPLC
Column Name:Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um)
Column Pressure:6000-10000
Column Temperature:50
Flow Gradient:Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 6. 22.00 0.4 99.0 1.0 5
Flow Rate:0.4 ml/min
Injection Temperature:8
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Randomization Order:Randomized
Chromatography Type:Reversed phase

MS:

MS ID:MS003560
Analysis ID:AN003818
Instrument Name:Thermo Q Exactive HF-X Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Instrument: Thermo Q Exactive HFx Software: Xcalibur 4.1.31.9 for data acquisition; Progenesis QI 2.4 for data preprocessing
Ion Mode:POSITIVE
Capillary Temperature:320 °C
Capillary Voltage:3.5 KV
Collision Energy:20-45, ramp
Collision Gas:N2
Dry Gas Flow:55
Dry Gas Temp:400°C
Fragmentation Method:CID
Desolvation Gas Flow:55
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