Summary of Study ST002437

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001569. The data can be accessed directly via it's Project DOI: 10.21228/M8WD9H This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002437
Study TitleMetabolomic analysis in wildtype and Isg15 knockout mice after sham or transverse aortic constriction surgery
Study SummaryLeft ventricular tissues were isolated from wildtype and Isg15 knockout mice 8 weeks after sham or transverse aortic constriction and the metabolomic differences were compared.
Institute
St. Michael's Hospital
Last NameAdvani
First NameAndrew
Address6-151, 61 Queen Street East, Toronto, Ontario. M5C 2t2
Emailandrew.advani@unityhealth.to
Phone4163604000
Submit Date2023-01-07
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2023-07-26
Release Version1
Andrew Advani Andrew Advani
https://dx.doi.org/10.21228/M8WD9H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001569
Project DOI:doi: 10.21228/M8WD9H
Project Title:Metabolomic analysis in wildtype and Isg15 knockout mice after sham or transverse aortic constriction surgery
Project Type:Untargeted metabolomic analysis
Project Summary:Left ventricular tissue was isolated from wildtype and Isg15 knockout mice 8 weeks after sham or transverse aortic constriction surgery and the metabolomic differences across the samples were compared.
Institute:St. Michael's Hospital
Department:Keenan Research Centre for Biomedical Science
Laboratory:Advani Lab
Last Name:Advani
First Name:Andrew
Address:6-151, 61 Queen St. East, Toronto, Ontario. M5C 2T2
Email:andrew.advani@unityhealth.to
Phone:4163604000

Subject:

Subject ID:SU002526
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype Surgical condition
SA243600Sample 10ISG15 KO Sham
SA243601Sample 7ISG15 KO Sham
SA243602Sample 8ISG15 KO Sham
SA243603Sample 6ISG15 KO Sham
SA243604Sample 18ISG15 KO TAC
SA243605Sample 16ISG15 KO TAC
SA243606Sample 17ISG15 KO TAC
SA243607Sample 19ISG15 KO TAC
SA243608Sample 2Wild type Sham
SA243609Sample 4Wild type Sham
SA243610Sample 3Wild type Sham
SA243611Sample 11Wild type TAC
SA243612Sample 12Wild type TAC
SA243613Sample 13Wild type TAC
SA243614Sample 14Wild type TAC
Showing results 1 to 15 of 15

Collection:

Collection ID:CO002519
Collection Summary:Mouse tissue frozen samples were reconstituted in 500 uL 80% MeCN containing 1 uM caffeine-d9 internal standard. The samples were homogenized, vortexed and centrifuged 21000 x g for two minutes at room temperature (23°C).
Sample Type:Cardiac tissue

Treatment:

Treatment ID:TR002538
Treatment Summary:Wildtype and ISG15 knockout mice underwent sham or transverse aortic constriction surgery.

Sample Preparation:

Sampleprep ID:SP002532
Sampleprep Summary:200 uL of each sample supernatants were diluted with 200 uL H2O and 100 uL used for the LC-MS analysis. LC-MS analyses were performed using Vanquish UPLC with 0-100% 10 minutes gradient (0.1% FA - 0.1%FA/MeCN) at 200 uL/min flow rate on Phenomenex Luna Omega 3 um Polar C18 100A 50x2.1 mm (RP-LC-MS). 10 uL injection volumes were used for each run. Mass spectra were acquired using Thermo Orbitrap QE+ spectrometer in positive and negative modes in separate runs.

Combined analysis:

Analysis ID AN003970 AN003971
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish
Column Phenomenex Luna Omega Polar C18(50 x 2.1mm, 3um. 100A) Phenomenex Luna Omega Polar C18(50 x 2.1mm, 3um. 100A)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak Area Peak Area

Chromatography:

Chromatography ID:CH002936
Chromatography Summary:LC-MS analyses were performed using Vanquish UPLC with 0-100% 10 minutes gradient (0.1% FA - 0.1%FA/MeCN) at 200 uL/min flow rate on Phenomenex Luna Omega 3 um Polar C18 100A 50x2.1 mm (RP-LC-MS). 10 uL injection volumes were used for each run.
Instrument Name:Thermo Vanquish
Column Name:Phenomenex Luna Omega Polar C18(50 x 2.1mm, 3um. 100A)
Column Temperature:23
Flow Gradient:0-100% 10 minutes gradient
Flow Rate:200 uL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS003704
Analysis ID:AN003970
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Mass spectra were acquired using Thermo Orbitrap QE+ spectrometer in positive and negative modes in separate runs. Data for each LC/acquisition type were analyzed using Compound Discoverer 3.3 (Thermo).
Ion Mode:POSITIVE
  
MS ID:MS003705
Analysis ID:AN003971
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Mass spectra were acquired using Thermo Orbitrap QE+ spectrometer in positive and negative modes in separate runs. Data for each LC/acquisition type were analyzed using Compound Discoverer 3.3 (Thermo).
Ion Mode:NEGATIVE
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