Summary of Study ST002443

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001574. The data can be accessed directly via it's Project DOI: 10.21228/M87Q6K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002443
Study TitleCharacterizing the intrauterine environment via untargeted metabolomics profiling of maternal blood collected during pregnancy (ARCH Cohort)
Study TypeProspective Cohort Study
Study SummaryThe ARCH Cohort is a pregnancy cohort of approximately 1,000 women recruited at the first prenatal visit largely in Lansing, MI between 2008 and 2016. Blood was collected when possible at the onset of prenatal care and at the time of the glucose tolerance test (late 2nd, early 3rd trimester). Please contact Jean Kerver at kerverje@msu.edu for questions related to the subject characteristics and outcomes. This research was supported by the Environmental influences on Child Health Outcomes (ECHO) OIF program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. ARCH is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375 ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382 North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539
Institute
Michigan State University
DepartmentDepartment of Epidemiology and Biostatistics
Last NameKerver
First NameJean
Address909 Wilson Road, East Lansing, MI, 48824
Emailkerverje@msu.edu
Phone517-290-5062
Submit Date2023-01-17
Total Subjects240
Study CommentsHHEAR Project EM19-0009, ECHO Project EC0374
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-01-16
Release Version1
Jean Kerver Jean Kerver
https://dx.doi.org/10.21228/M87Q6K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001574
Project DOI:doi: 10.21228/M87Q6K
Project Title:Characterizing the intrauterine environment via untargeted metabolomics profiling of maternal blood collected during pregnancy (ARCH Cohort)
Project Type:C18 Reversed-Phase Broad Spectrum Metabolomics
Project Summary:This project is funded by the NIH Environmental influences on Child Health Outcomes (ECHO) program, and uses data from five cohorts: Healthy Start, Project Viva, Atlanta ECHO, ARCH Cohort, and PETALS. This project will generate new untargeted metabolomics profiling data at two time-points in pregnancy for all women in order to identify trimester-specific metabolomics signatures and longitudinal metabolomics trajectories associated with offspring birth size; identify the contribution of maternal sociodemographic and perinatal characteristics to the metabolomics signatures.
Institute:NC HHEAR Hub
Department:Untargeted Analysis
Laboratory:Sumner Lab
Last Name:Li
First Name:Yuanyuan
Address:Nutrition Research Institute, UNC-CH, 500 Laureate Way, Kannapolis, NC 28081
Email:yuanyli4@unc.edu
Phone:984-377-0693
Funding Source:This research was supported by the Environmental influences on Child Health Outcomes (ECHO) program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The ARCH ECHO Cohort is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375; ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382; North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857; Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539

Subject:

Subject ID:SU002532
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample Type
SA244277PZPa_HHEAR_18HHEAR Pool
SA244278PZPa_HHEAR_17HHEAR Pool
SA244279PZPa_HHEAR_16HHEAR Pool
SA244280PZPa_HHEAR_19HHEAR Pool
SA244281PZPa_HHEAR_15HHEAR Pool
SA244282PZPa_HHEAR_21HHEAR Pool
SA244283PZPa_HHEAR_1HHEAR Pool
SA244284PZPa_HHEAR_24HHEAR Pool
SA244285PZPa_HHEAR_22HHEAR Pool
SA244286PZPa_HHEAR_14HHEAR Pool
SA244287PZPa_HHEAR_20HHEAR Pool
SA244288PZPa_HHEAR_23HHEAR Pool
SA244289PZPa_HHEAR_2HHEAR Pool
SA244290PZPa_HHEAR_5HHEAR Pool
SA244291PZPa_HHEAR_4HHEAR Pool
SA244292PZPa_HHEAR_3HHEAR Pool
SA244293PZPa_HHEAR_13HHEAR Pool
SA244294PZPa_HHEAR_7HHEAR Pool
SA244295PZPa_HHEAR_6HHEAR Pool
SA244296PZPa_HHEAR_12HHEAR Pool
SA244297PZPa_HHEAR_10HHEAR Pool
SA244298PZPa_HHEAR_11HHEAR Pool
SA244299PZPa_HHEAR_9HHEAR Pool
SA244300PZPa_HHEAR_8HHEAR Pool
SA244301PZPa_NIST_7NIST Pool
SA244302PZPa_NIST_9NIST Pool
SA244303PZPa_NIST_6NIST Pool
SA244304PZPa_NIST_8NIST Pool
SA244305PZPa_NIST_5NIST Pool
SA244306PZPa_NIST_4NIST Pool
SA244307PZPa_NIST_1NIST Pool
SA244308PZPa_NIST_2NIST Pool
SA244309PZPa_NIST_3NIST Pool
SA244310PZPa_SP_8Study Pool
SA244311PZPa_SP_9Study Pool
SA244312PZPa_SP_11Study Pool
SA244313PZPa_SP_7Study Pool
SA244314PZPa_SP_10Study Pool
SA244315PZPa_SP_4Study Pool
SA244316PZPa_SP_12Study Pool
SA244317PZPa_SP_1Study Pool
SA244318PZPa_SP_2Study Pool
SA244319PZPa_SP_5Study Pool
SA244320PZPa_SP_6Study Pool
SA244321PZPa_SP_3Study Pool
SA244322PZPa_SP_21Study Pool
SA244323PZPa_SP_20Study Pool
SA244324PZPa_SP_22Study Pool
SA244325PZPa_SP_13Study Pool
SA244326PZPa_SP_24Study Pool
SA244327PZPa_SP_19Study Pool
SA244328PZPa_SP_23Study Pool
SA244329PZPa_SP_18Study Pool
SA244330PZPa_SP_16Study Pool
SA244331PZPa_SP_15Study Pool
SA244332PZPa_SP_14Study Pool
SA244333PZPa_SP_17Study Pool
SA244334PZPa_S_169Study Sample
SA244335PZPa_S_171Study Sample
SA244336PZPa_S_172Study Sample
SA244337PZPa_S_168Study Sample
SA244338PZPa_S_170Study Sample
SA244339PZPa_S_166Study Sample
SA244340PZPa_S_173Study Sample
SA244341PZPa_S_162Study Sample
SA244342PZPa_S_163Study Sample
SA244343PZPa_S_164Study Sample
SA244344PZPa_S_165Study Sample
SA244345PZPa_S_167Study Sample
SA244346PZPa_S_179Study Sample
SA244347PZPa_S_183Study Sample
SA244348PZPa_S_182Study Sample
SA244349PZPa_S_184Study Sample
SA244350PZPa_S_185Study Sample
SA244351PZPa_S_186Study Sample
SA244352PZPa_S_181Study Sample
SA244353PZPa_S_180Study Sample
SA244354PZPa_S_176Study Sample
SA244355PZPa_S_175Study Sample
SA244356PZPa_S_177Study Sample
SA244357PZPa_S_178Study Sample
SA244358PZPa_S_161Study Sample
SA244359PZPa_S_174Study Sample
SA244360PZPa_S_154Study Sample
SA244361PZPa_S_142Study Sample
SA244362PZPa_S_141Study Sample
SA244363PZPa_S_143Study Sample
SA244364PZPa_S_144Study Sample
SA244365PZPa_S_145Study Sample
SA244366PZPa_S_140Study Sample
SA244367PZPa_S_139Study Sample
SA244368PZPa_S_135Study Sample
SA244369PZPa_S_187Study Sample
SA244370PZPa_S_136Study Sample
SA244371PZPa_S_137Study Sample
SA244372PZPa_S_138Study Sample
SA244373PZPa_S_146Study Sample
SA244374PZPa_S_147Study Sample
SA244375PZPa_S_156Study Sample
SA244376PZPa_S_155Study Sample
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Collection:

Collection ID:CO002525
Collection Summary:The ARCH Cohort is a pregnancy cohort of approximately 1,000 women recruited at the first prenatal visit largely in Lansing, MI between 2008 and 2016. Blood was collected when possible at the onset of prenatal care and at the time of the glucose tolerance test (late 2nd, early 3rd trimester). Please contact Jean Kerver at kerverje@msu.edu for questions related to the subject characteristics and outcomes.
Sample Type:Blood (plasma)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002544
Treatment Summary:N/A

Sample Preparation:

Sampleprep ID:SP002538
Sampleprep Summary:Study samples and study pools (50 µL aliquots) were shipped from Michigan State University to the NC HHEAR Hub on dry ice. HHEAR reference plasma (50 µL each) and NIST plasma (1950) reference material (50 µL each) were provided by the NC HHEAR Hub. LC-MS grade water (50 µL) was used as blank. All samples were thawed at 4°C overnight before the preparation. Samples, including study samples, study pool samples, HHEAR reference plasma, NIST reference plasma, and blanks were mixed with 400 µL methanol containing 500 ng/mL tryptophan-d5 as internal standard and vortexed by a multiple tube vortex mixer for 2 min at 5000 rpm at room temperature. All samples were centrifuged at 16,000 rcf for 10 min at 4°C. The supernatant (350 µL) was transferred into a pre-labeled 2.0 mL Lo-bind Eppendorf tube, dried by a SpeedVac overnight, and stored at -80° C. For immediate analysis, 100 µL of water-methanol solution (95:5, v/v) was used to reconstitute the dried extracts. Samples were thoroughly mixed on a multiple tube vortex mixer for 10 min at 5000 rpm at room temperature and then centrifuged at 4°C for 10 min at 16,000 rcf. The supernatant was transferred to pre-labeled autosampler vials for data acquisition by LC-MS.
Processing Storage Conditions:On ice
Extraction Method:Vortex with methanol containing 500ng/ml tryptophan-d5 as internal standard
Extract Storage:-80℃
Sample Resuspension:Water-Methanol (95:5, v/v)
Sample Spiking:Tryptophan-d5 stock solution at 500 ng/mL

Combined analysis:

Analysis ID AN003980
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF-X Orbitrap
Ion Mode POSITIVE
Units Peak area

Chromatography:

Chromatography ID:CH002943
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
Column Pressure:6000-10000 psi
Column Temperature:50
Flow Gradient:Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 6. 22.00 0.4 99.0 1.0 5
Flow Rate:0.4 mL/min
Injection Temperature:8
Internal Standard:Tryptophan-d5
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Analytical Time:22 min
Weak Wash Solvent Name:10% methanol/90% water; 0.1% formic acid
Strong Wash Solvent Name:75% 2-Propanol/25% Water; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS003714
Analysis ID:AN003980
Instrument Name:Thermo Q Exactive HF-X Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Xcalibur 4.1.31.9 for data acquisition; Progenesis QI 2.4 for data preprocessing
Ion Mode:POSITIVE
Capillary Temperature:320 °C
Capillary Voltage:3.5 kV
Collision Energy:20-45, ramp
Collision Gas:N2
Dry Gas Temp:400°C
Fragmentation Method:CID
Desolvation Gas Flow:55
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