Summary of Study ST002626

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002626
Study TitleMoTrPAC: Endurance exercise training study in young adult rats, Tissue:White Adipose - Targeted Oxylipins
Study SummaryThe goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute
Emory University
DepartmentBiochemistry
LaboratoryIntegrated Lipidomics and Metabolomics Core
Last NameOrtlund
First NameEric
Address1510 Clifton Rd. NE, Room G235, Atlanta, GA 30322
Emaileortlun@emory.edu
Phone(404) 727-5014
Submit Date2023-04-25
Raw Data AvailableYes
Raw Data File Type(s)TBD
Analysis Type DetailLC-MS
Release Date2023-10-06
Release Version1
Eric Ortlund Eric Ortlund
https://dx.doi.org/10.21228/M8V97D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001020
Project DOI:doi: 10.21228/M8V97D
Project Title:MoTrPAC
Project Summary:MoTrPAC is a national research consortium designed to discover and perform preliminary characterization of the range of molecular transducers (the "molecular map") that underlie the effects of physical activity in humans. The program's goal is to study the molecular changes that occur during and after exercise and ultimately to advance the understanding of how physical activity improves and preserves health. Preclinical and clinical studies will examine the systemic effects of endurance and resistance exercise across a range of ages and fitness levels by molecular probing of multiple tissues before and after acute and chronic exercise. This program is the largest targeted NIH investment of funds into the mechanisms of how physical activity improves health and prevents disease. The MoTrPAC program is supported by the NIH Common Fund and is managed by a trans-agency working group representing multiple NIH institutes and centers, led by the NIH Office of Strategic Coordination, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute on Aging, and National Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee: Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac Bioinformatics / Data Coordination Center.
Institute:MoTrPAC
Last Name:Ashley
First Name:Euan
Address:Falk Building CV267, 870 Quarry Road, Stanford, California 94305
Email:motrpac-data-deposition@lists.stanford.edu
Phone:(650) 725-1846

Subject:

Subject ID:SU002728
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Timepoint Sex
SA26156590266017010Control 8 weeks of training or control time Female
SA26156690245017010Control 8 weeks of training or control time Female
SA26156790265017010Control 8 weeks of training or control time Female
SA26156890252017010Control 8 weeks of training or control time Female
SA26156990248017010Control 8 weeks of training or control time Female
SA26157090217017010Control 8 weeks of training or control time Male
SA26157190239017010Control 8 weeks of training or control time Male
SA26157290232017010Control 8 weeks of training or control time Male
SA26157390229017010Control 8 weeks of training or control time Male
SA26157490237017010Control 8 weeks of training or control time Male
SA261575QC_blank_65QC-Blank 0 hour -
SA261576QC_blank_68QC-Blank 0 hour -
SA261577QC_blank_78QC-Blank 0 hour -
SA261578QC_blank_5QC-Blank 0 hour -
SA261579QC_blank_79QC-Blank 0 hour -
SA261580QC_blank_69QC-Blank 0 hour -
SA261581QC_Standard_70QC-ExternalStandard 0 hour -
SA261582QC_Standard_73QC-ExternalStandard 0 hour -
SA261583QC_Standard_74QC-ExternalStandard 0 hour -
SA261584QC_Standard_76QC-ExternalStandard 0 hour -
SA261585QC_Standard_72QC-ExternalStandard 0 hour -
SA261586QC_Standard_77QC-ExternalStandard 0 hour -
SA261587QC_Standard_71QC-ExternalStandard 0 hour -
SA261588QC_Standard_75QC-ExternalStandard 0 hour -
SA261589QC_62QC-Pooled 0 hour -
SA261590QC_67QC-Pooled 0 hour -
SA261591QC_51QC-Pooled 0 hour -
SA261592QC_40QC-Pooled 0 hour -
SA261593QC_18QC-Pooled 0 hour -
SA261594QC_7QC-Pooled 0 hour -
SA261595QC_29QC-Pooled 0 hour -
SA261596QC_prerun_1QC-PreRun 0 hour -
SA261597QC_prerun_3QC-PreRun 0 hour -
SA261598QC_prerun_2QC-PreRun 0 hour -
SA261599QC_SRMQC-Reference 0 hour -
SA261600G_QC_2_64QC-Reference 0 hour -
SA261601G_QC_2_6QC-Reference 0 hour -
SA261602G_QC_1_4QC-Reference 0 hour -
SA261603G_QC_1_63QC-Reference 0 hour -
SA26160490571017010Training 1 week of training or control time Female
SA26160590559017010Training 1 week of training or control time Female
SA26160690567017010Training 1 week of training or control time Female
SA26160790564017010Training 1 week of training or control time Female
SA26160890560017010Training 1 week of training or control time Female
SA26160990423017010Training 1 week of training or control time Male
SA26161090421017010Training 1 week of training or control time Male
SA26161190430017010Training 1 week of training or control time Male
SA26161290422017010Training 1 week of training or control time Male
SA26161390426017010Training 1 week of training or control time Male
SA26161490581017010Training 2 weeks of training Female
SA26161590587017010Training 2 weeks of training Female
SA26161690578017010Training 2 weeks of training Female
SA26161790576017010Training 2 weeks of training Female
SA26161890585017010Training 2 weeks of training Female
SA26161990450017010Training 2 weeks of training Male
SA26162090444017010Training 2 weeks of training Male
SA26162190439017010Training 2 weeks of training Male
SA26162290441017010Training 2 weeks of training Male
SA26162390449017010Training 2 weeks of training Male
SA26162490412017010Training 4 weeks of training Female
SA26162590420017010Training 4 weeks of training Female
SA26162690406017010Training 4 weeks of training Female
SA26162790410017010Training 4 weeks of training Female
SA26162890416017010Training 4 weeks of training Female
SA26162990281017010Training 4 weeks of training Male
SA26163090283017010Training 4 weeks of training Male
SA26163190289017010Training 4 weeks of training Male
SA26163290280017010Training 4 weeks of training Male
SA26163390292017010Training 4 weeks of training Male
SA26163490251017010Training 8 weeks of training or control time Female
SA26163590254017010Training 8 weeks of training or control time Female
SA26163690267017010Training 8 weeks of training or control time Female
SA26163790259017010Training 8 weeks of training or control time Female
SA26163890258017010Training 8 weeks of training or control time Female
SA26163990223017010Training 8 weeks of training or control time Male
SA26164090222017010Training 8 weeks of training or control time Male
SA26164190218017010Training 8 weeks of training or control time Male
SA26164290225017010Training 8 weeks of training or control time Male
SA26164390227017010Training 8 weeks of training or control time Male
Showing results 1 to 79 of 79

Collection:

Collection ID:CO002721
Collection Summary:-
Sample Type:White adipose

Treatment:

Treatment ID:TR002737
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP002734
Sampleprep Summary:purification of oxylipids with SPE column
Sampleprep Protocol Filename:pass1b_experimental_design_metabolomics.pdf

Combined analysis:

Analysis ID AN004298
Analysis type MS
Chromatography type Reversed phase
Chromatography system ExionLC AC
Column ThermoScientific Accucore C18 (100 x 4.6mm, 2.6um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 5500 Qtrap
Ion Mode NEGATIVE
Units ng/uL

Chromatography:

Chromatography ID:CH003204
Chromatography Summary:Lipids were separated by liquid chromatography with 20 minutes gradient
Methods Filename:pass1b_oxylipneg_etamidpos_methods.pdf
Instrument Name:ExionLC AC
Column Name:ThermoScientific Accucore C18 (100 x 4.6mm, 2.6um)
Column Temperature:50C
Flow Gradient:t=0 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=0.5 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=1 min: 50%/50% (%A/%B) @ 0.5 ml/min | t=2 min: 50%/50% (%A/%B) @ 0.5 ml/min | t=2.1 min: 25%/75% (%A/%B) @ 0.5 ml/min | t=5 min: 25%/75% (%A/%B) @ 0.5 ml/min | t=7 min: 15%/85% (%A/%B) @ 0.5 ml/min | t=12 min: 15%/85% (%A/%B) @ 0.5 ml/min | t=12.1 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=18 min: 90%/10% (%A/%B) @ 0.5 ml/min
Flow Rate:0.5 ml/min
Solvent A:100% water; 10 mM ammonium acetate
Solvent B:100% acetonitrile; 10 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS004045
Analysis ID:AN004298
Instrument Name:ABI Sciex 5500 Qtrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Multiple reaction monitoring mode (MRM)
Ion Mode:NEGATIVE
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