Summary of Study ST003142

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001953. The data can be accessed directly via it's Project DOI: 10.21228/M88M77 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003142
Study TitleEffect of liver Acox1 knockout on serum lipidome in mice
Study SummaryThe liver gene expression of the peroxisomal β-oxidation enzyme acyl-coenzyme A oxidase 1 (ACOX1), which catabolizes very long chain fatty acids (VLCFA), increases in the context of obesity. To check if liver peroxisomal fatty acids beta-oxidation deficiency will affect whole body metabolic homeostasis through circulating lipids. We analyzed serum samples from 5 WT and 5 Acox1-LKO mice.
Institute
Washington University in St. Louis
Last NameLu
First NameDongliang
Address660 S. Euclid Ave.
Emailludong-liang@wustl.edu
Phone3147476766
Submit Date2024-03-19
Analysis Type DetailLC-MS
Release Date2024-03-27
Release Version1
Dongliang Lu Dongliang Lu
https://dx.doi.org/10.21228/M88M77
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001953
Project DOI:doi: 10.21228/M88M77
Project Title:Global Lipidomics of Serum Samples from control and ACOX1-LKO Mouse
Project Summary:In this study, we use a Acox1 liver specific knock mouse to explore the role of liver peroxisomal fatty acids beta-oxidation in whole body metabolic homeostasis. As the key enzyme of peroxisomal fatty acid beta-oxidation, knock out Acox1 in liver will affect the very long chain fatty acid accoumaltion in liver and their secretion into circulating.
Institute:Washington University School of Medicine
Last Name:Lu
First Name:Dongliang
Address:660 S. Euclid Ave., St. Louis, Missouri, 63110, USA
Email:ludong-liang@wustl.edu
Phone:3147476766

Subject:

Subject ID:SU003259
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample_ID Sample source
SA340618Sample8Acox1-LKO mouse serum
SA340619Sample9Acox1-LKO mouse serum
SA340620Sample10Acox1-LKO mouse serum
SA340621Sample7Acox1-LKO mouse serum
SA340622Sample6Acox1-LKO mouse serum
SA340623Sample2Wild-type mouse serum
SA340624Sample3Wild-type mouse serum
SA340625Sample4Wild-type mouse serum
SA340626Sample5Wild-type mouse serum
SA340627Sample1Wild-type mouse serum
Showing results 1 to 10 of 10

Collection:

Collection ID:CO003252
Collection Summary:After collecting the whole blood of mice, allow the blood to clot by leaving at room temperature for 30 minutes. Then serum were collected by centrifugation at 2000 g for 10 minutes, and save at -80C
Sample Type:Blood (serum)

Treatment:

Treatment ID:TR003268
Treatment Summary:WT and Acox1-LKO mice were maintained under constant temperature (24°C), circulating air and humidity (45-65%) with 12h:12h light/dark cycle. All mice had free access to chew diet and water. 10 weeks age mice were used for analysis.

Sample Preparation:

Sampleprep ID:SP003266
Sampleprep Summary:The lipids extraction was performed strictly following the SOP established based on a modified Folch liquid-liquid extraction protocol. Briefly, an aliquot of 4.5 μL of each sample was vortexed with 1.5 μL of internal standard solution and methanol, followed by adding dichloromethane and vortexing for another 20 s. A clean-up step was performed with water and 10 seconds of vortex. Samples were allowed to equilibrate at room temperature for 10 min and centrifuged at 16,000 g for 10 min at 4°C. An aliquot of the organic layer was evaporated to dryness with a nitrogen blowdown evaporator. The residue was re-suspended in 4.5 μL of NovaMT MixB, vortexed for 1 min, and diluted with 40.5 μL of NovaMT MixA

Combined analysis:

Analysis ID AN005155 AN005156
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Bruker Impact HD Bruker Impact HD
Ion Mode POSITIVE NEGATIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH003903
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um)
Column Temperature:42 °C
Flow Gradient:t = 0 min, 5% B; t = 10 min, 40% B; t = 18.8 min, 98% B; t = 20.5 min, 98% B.
Flow Rate:250 μL/min.
Solvent A:50% Methanol/40% Acetonitrile/10% Water; 10 mM Ammonium formate
Solvent B:95% Isopropyl alcohol/5% water; 10 mM Ammonium formate
Chromatography Type:Reversed phase

MS:

MS ID:MS004891
Analysis ID:AN005155
Instrument Name:Bruker Impact HD
Instrument Type:QTOF
MS Type:ESI
MS Comments:The acquisition rate was 1.44 Hz for MS acquisition and 4 – 10 Hz for MS/MS spectra acquisition, with an m/z range from 150 to 1500. For both positive and negative ionization. Intensity threshold:2500 cts for positive ionization. Lipid features were extracted and aligned using software LipidScreener 1.1.0
Ion Mode:POSITIVE
Capillary Voltage:4200 V
Collision Energy:10-70 eV
Dry Gas Flow:5.0 L/min
Dry Gas Temp:240°C
Nebulizer:1.2 Bar
  
MS ID:MS004892
Analysis ID:AN005156
Instrument Name:Bruker Impact HD
Instrument Type:QTOF
MS Type:ESI
MS Comments:The acquisition rate was 1.44 Hz for MS acquisition and 4 – 10 Hz for MS/MS spectra acquisition, with an m/z range from 150 to 1500. For both positive and negative ionization. Intensity threshold:1500 cts for negative ionization. Lipid features were extracted and aligned using software LipidScreener 1.1.0
Ion Mode:NEGATIVE
Capillary Voltage:4200 V
Collision Energy:10-70 eV
Dry Gas Flow:5.0 L/min
Dry Gas Temp:240°C
Nebulizer:1.2 Bar
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