Summary of Study ST001292
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000874. The data can be accessed directly via it's Project DOI: 10.21228/M8Q975 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001292 |
| Study Title | Metabolomics of blood plasma and kidney tissue from control (db/m) and diabetic (db/db) mice. |
| Study Type | MS/MSMS analysis |
| Study Summary | Plasma and kidney from control (db/m) and diabetic (db/db) mice on regular or eNOS -/- background. Some mice received treatment (20 mg/kg/d lisinopril + 30 mg/kg/d losartan) for 12 wk (starting at 12 wk age). Samples paired (plasma/tissue from individual mice). Samples snap frozen and stored at -80 until use. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 1000 wall St., Ann Arbor, MI |
| mkachman@med.umich.edu | |
| Phone | 734-232-0842 |
| Submit Date | 2019-12-16 |
| Study Comments | The wrong mouse number was recorded for P_29 and P_59 so they were excluded from analysis. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-12-18 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000874 |
| Project DOI: | doi: 10.21228/M8Q975 |
| Project Title: | Metabolomics of blood plasma and kidney tissue from control (db/m) and diabetic (db/db) mice. |
| Project Type: | MS/MSMS analysis |
| Project Summary: | Determine lipids in plasma and microvascular complication prone tissues in diabetic mouse models |
| Institute: | Michigan Center for Translational Pathology |
| Department: | UMich Internal Medicine |
| Laboratory: | Pennathur Lab |
| Last Name: | Pennathur |
| First Name: | Subramaniam |
| Address: | University Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105 |
| Email: | spennath@umich.edu |
| Phone: | 734 615 5757 |
Subject:
| Subject ID: | SU001366 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | ACE/ARB Inhibitor |
|---|---|---|
| SA093910 | S00030167 | No Treat | control/diabetic:db/db diabetic | eNOS background:eNOS -/- |
| SA093911 | S00030117 | No Treat | control/diabetic:db/db diabetic | eNOS background:eNOS -/- |
| SA093912 | S00030152 | No Treat | control/diabetic:db/db diabetic | eNOS background:eNOS -/- |
| SA093913 | S00030131 | No Treat | control/diabetic:db/db diabetic | eNOS background:eNOS -/- |
| SA093914 | S00030159 | No Treat | control/diabetic:db/db diabetic | eNOS background:eNOS -/- |
| SA093915 | S00030098 | No Treat | control/diabetic:db/db diabetic | eNOS background:eNOS -/- |
| SA093916 | S00030146 | No Treat | control/diabetic:db/db diabetic | eNOS background:eNOS -/- |
| SA093917 | S00030139 | No Treat | control/diabetic:db/db diabetic | eNOS background:eNOS -/- |
| SA093918 | S00030110 | No Treat | control/diabetic:db/db diabetic | eNOS background:eNOS -/- |
| SA093919 | S00030170 | No Treat | control/diabetic:db/db diabetic | eNOS background:eNOS -/- |
| SA093900 | S00030150 | No Treat | control/diabetic:db/db diabetic | eNOS background:eNOS +/+ |
| SA093901 | S00030144 | No Treat | control/diabetic:db/db diabetic | eNOS background:eNOS +/+ |
| SA093902 | S00030128 | No Treat | control/diabetic:db/db diabetic | eNOS background:eNOS +/+ |
| SA093903 | S00030157 | No Treat | control/diabetic:db/db diabetic | eNOS background:eNOS +/+ |
| SA093904 | S00030166 | No Treat | control/diabetic:db/db diabetic | eNOS background:eNOS +/+ |
| SA093905 | S00030173 | No Treat | control/diabetic:db/db diabetic | eNOS background:eNOS +/+ |
| SA093906 | S00030171 | No Treat | control/diabetic:db/db diabetic | eNOS background:eNOS +/+ |
| SA093907 | S00030121 | No Treat | control/diabetic:db/db diabetic | eNOS background:eNOS +/+ |
| SA093908 | S00030164 | No Treat | control/diabetic:db/db diabetic | eNOS background:eNOS +/+ |
| SA093909 | S00030136 | No Treat | control/diabetic:db/db diabetic | eNOS background:eNOS +/+ |
| SA093930 | S00030119 | No Treat | control/diabetic:db/m control | eNOS background:eNOS -/- |
| SA093931 | S00030099 | No Treat | control/diabetic:db/m control | eNOS background:eNOS -/- |
| SA093932 | S00030172 | No Treat | control/diabetic:db/m control | eNOS background:eNOS -/- |
| SA093933 | S00030112 | No Treat | control/diabetic:db/m control | eNOS background:eNOS -/- |
| SA093934 | S00030154 | No Treat | control/diabetic:db/m control | eNOS background:eNOS -/- |
| SA093935 | S00030141 | No Treat | control/diabetic:db/m control | eNOS background:eNOS -/- |
| SA093936 | S00030161 | No Treat | control/diabetic:db/m control | eNOS background:eNOS -/- |
| SA093937 | S00030133 | No Treat | control/diabetic:db/m control | eNOS background:eNOS -/- |
| SA093938 | S00030125 | No Treat | control/diabetic:db/m control | eNOS background:eNOS -/- |
| SA093939 | S00030105 | No Treat | control/diabetic:db/m control | eNOS background:eNOS -/- |
| SA093920 | S00030129 | No Treat | control/diabetic:db/m control | eNOS background:eNOS +/+ |
| SA093921 | S00030158 | No Treat | control/diabetic:db/m control | eNOS background:eNOS +/+ |
| SA093922 | S00030151 | No Treat | control/diabetic:db/m control | eNOS background:eNOS +/+ |
| SA093923 | S00030137 | No Treat | control/diabetic:db/m control | eNOS background:eNOS +/+ |
| SA093924 | S00030145 | No Treat | control/diabetic:db/m control | eNOS background:eNOS +/+ |
| SA093925 | S00030103 | No Treat | control/diabetic:db/m control | eNOS background:eNOS +/+ |
| SA093926 | S00030108 | No Treat | control/diabetic:db/m control | eNOS background:eNOS +/+ |
| SA093927 | S00030115 | No Treat | control/diabetic:db/m control | eNOS background:eNOS +/+ |
| SA093928 | S00030165 | No Treat | control/diabetic:db/m control | eNOS background:eNOS +/+ |
| SA093929 | S00030122 | No Treat | control/diabetic:db/m control | eNOS background:eNOS +/+ |
| SA093950 | S00030116 | Treat | control/diabetic:db/db diabetic | eNOS background:eNOS -/- |
| SA093951 | S00030130 | Treat | control/diabetic:db/db diabetic | eNOS background:eNOS -/- |
| SA093952 | S00030174 | Treat | control/diabetic:db/db diabetic | eNOS background:eNOS -/- |
| SA093953 | S00030169 | Treat | control/diabetic:db/db diabetic | eNOS background:eNOS -/- |
| SA093954 | S00030138 | Treat | control/diabetic:db/db diabetic | eNOS background:eNOS -/- |
| SA093955 | S00030109 | Treat | control/diabetic:db/db diabetic | eNOS background:eNOS -/- |
| SA093956 | S00030123 | Treat | control/diabetic:db/db diabetic | eNOS background:eNOS -/- |
| SA093940 | S00030148 | Treat | control/diabetic:db/db diabetic | eNOS background:eNOS +/+ |
| SA093941 | S00030162 | Treat | control/diabetic:db/db diabetic | eNOS background:eNOS +/+ |
| SA093942 | S00030168 | Treat | control/diabetic:db/db diabetic | eNOS background:eNOS +/+ |
| SA093943 | S00030102 | Treat | control/diabetic:db/db diabetic | eNOS background:eNOS +/+ |
| SA093944 | S00030155 | Treat | control/diabetic:db/db diabetic | eNOS background:eNOS +/+ |
| SA093945 | S00030142 | Treat | control/diabetic:db/db diabetic | eNOS background:eNOS +/+ |
| SA093946 | S00030126 | Treat | control/diabetic:db/db diabetic | eNOS background:eNOS +/+ |
| SA093947 | S00030134 | Treat | control/diabetic:db/db diabetic | eNOS background:eNOS +/+ |
| SA093948 | S00030113 | Treat | control/diabetic:db/db diabetic | eNOS background:eNOS +/+ |
| SA093949 | S00030106 | Treat | control/diabetic:db/db diabetic | eNOS background:eNOS +/+ |
| SA093967 | S00030111 | Treat | control/diabetic:db/m control | eNOS background:eNOS -/- |
| SA093968 | S00030100 | Treat | control/diabetic:db/m control | eNOS background:eNOS -/- |
| SA093969 | S00030160 | Treat | control/diabetic:db/m control | eNOS background:eNOS -/- |
| SA093970 | S00030140 | Treat | control/diabetic:db/m control | eNOS background:eNOS -/- |
| SA093971 | S00030147 | Treat | control/diabetic:db/m control | eNOS background:eNOS -/- |
| SA093972 | S00030153 | Treat | control/diabetic:db/m control | eNOS background:eNOS -/- |
| SA093973 | S00030132 | Treat | control/diabetic:db/m control | eNOS background:eNOS -/- |
| SA093974 | S00030124 | Treat | control/diabetic:db/m control | eNOS background:eNOS -/- |
| SA093975 | S00030104 | Treat | control/diabetic:db/m control | eNOS background:eNOS -/- |
| SA093976 | S00030118 | Treat | control/diabetic:db/m control | eNOS background:eNOS -/- |
| SA093957 | S00030127 | Treat | control/diabetic:db/m control | eNOS background:eNOS +/+ |
| SA093958 | S00030114 | Treat | control/diabetic:db/m control | eNOS background:eNOS +/+ |
| SA093959 | S00030120 | Treat | control/diabetic:db/m control | eNOS background:eNOS +/+ |
| SA093960 | S00030163 | Treat | control/diabetic:db/m control | eNOS background:eNOS +/+ |
| SA093961 | S00030156 | Treat | control/diabetic:db/m control | eNOS background:eNOS +/+ |
| SA093962 | S00030149 | Treat | control/diabetic:db/m control | eNOS background:eNOS +/+ |
| SA093963 | S00030143 | Treat | control/diabetic:db/m control | eNOS background:eNOS +/+ |
| SA093964 | S00030107 | Treat | control/diabetic:db/m control | eNOS background:eNOS +/+ |
| SA093965 | S00030135 | Treat | control/diabetic:db/m control | eNOS background:eNOS +/+ |
| SA093966 | S00030101 | Treat | control/diabetic:db/m control | eNOS background:eNOS +/+ |
| Showing results 1 to 77 of 77 |
Collection:
| Collection ID: | CO001361 |
| Collection Summary: | Kidney samples snap-frozen and stored at -80 until use. |
| Sample Type: | Kidney |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001381 |
| Treatment Summary: | Treatment group mice received 20 mg/kg/d lisinopril + 30 mg/kg/d losartan) for 12 wk (starting at 12 wk age), controls didn't receive any drugs. |
Sample Preparation:
| Sampleprep ID: | SP001374 |
| Sampleprep Summary: | Standard tissue lipidomics protocol |
| Sampleprep Protocol Filename: | A004_-_Shotgun_Lipidomics.pdf |
Combined analysis:
| Analysis ID | AN002149 | AN002150 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Shimadzu Nexera X2 | Shimadzu Nexera X2 |
| Column | Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH001572 |
| Instrument Name: | Shimadzu Nexera X2 |
| Column Name: | Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001573 |
| Instrument Name: | Shimadzu Nexera X2 |
| Column Name: | Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002000 |
| Analysis ID: | AN002149 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | MSMS acquisition followed by LipidBLAST identification and targeted data extraction from MS1 trace for identified lipids |
| Ion Mode: | POSITIVE |
| MS ID: | MS002001 |
| Analysis ID: | AN002150 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | MSMS acquisition followed by LipidBLAST identification and targeted data extraction from MS1 trace for identified lipids |
| Ion Mode: | NEGATIVE |
