Summary of Study ST002783
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001736. The data can be accessed directly via it's Project DOI: 10.21228/M89Q7K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002783 |
Study Title | Metabolic changes of the pig kidney during isolated normothermic perfusion with autologous whole blood - perfusate |
Study Type | Experimental |
Study Summary | This study investigates how glucose, lactate and 20 amino acids in the perfusate of normothermically perfused pig kidneys changes over time. It also studies how type and severity of ischemia before perfusion (cold or warm ischemia) change this profile. |
Institute | KU Leuven |
Department | Microbiology, Immunology and Transplantation |
Laboratory | Lab of Abdominal Transplantation |
Last Name | Jochmans |
First Name | Ina |
Address | Herestraat 49, Leuven, 3000, Belgium |
ina.jochmans@kuleuven.be | |
Phone | None |
Submit Date | 2023-07-17 |
Num Groups | 3 |
Total Subjects | 18 kidneys |
Num Males | 9 male pigs |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2023-08-07 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004530 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Agilent 1290 Infinity |
Column | Agilent InfinityLab Poroshell 120 (2.1 x 150 mm, 2.7 um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Focus |
Ion Mode | NEGATIVE |
Units | Peak area |
Chromatography:
Chromatography ID: | CH003403 |
Chromatography Summary: | 10 µl was loaded onto an Ultra Performance Liquid Chromatograph (UPLC) equipped with a Hydrophilic Interaction Liquid Chromatography (HILIC) column (InfinityLab Poroshell 120 HILIC-Z PEEK-lined 2.1 x 150 mm, 2.7 µm column; Agilent, Santa Clara, USA)) and connected in-line to a Q-exactive Orbitrap Focus (Thermo Fisher Scientific, Massachusetts, USA) mass spectrometer. A linear gradient was built up starting with 90% solvent A (LC-MS grade acetonitrile, Acetonitrile hypergrade for LC-MS LiChrosolv, Supelco (Merck), Germany) and 10% solvent B (10 mM ammonium acetate (LiChropur™, eluent additive for LC-MS, (Merck), Germany), pH 9.3). At 2 min the gradient increased to 60% of solvent B and maintained at 60% until 15 min. The gradient returned to 10% solvent B at 16 min and remained until 25 min. The flow rate was 250 µl/min and the column was kept at 25°C throughout the analysis. The MS operated in negative ion mode, with a spray voltage of 2.9 kV and a temperature of the capillary of 325 °C. Gas settings were as follows: sheath gas 40 and auxiliary gas 15. The vaporizer temperature was set at 300 °C. A full scan (resolution of 70.000 and scan range of m/z 70-1050) was applied. XCalibur version to operate the LC-MS was 4.2.47. |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Agilent InfinityLab Poroshell 120 (2.1 x 150 mm, 2.7 um) |
Column Temperature: | 25°C |
Flow Gradient: | A linear gradient was built up starting with 90% solvent A and 10% solvent B. At 2 min the gradient increased to 60% of solvent B and maintained at 60% until 15 min. |
Flow Rate: | 250 µl/min |
Solvent A: | 100% acetonitrile |
Solvent B: | 100% water; 10 mM ammonium acetate, pH 9.3 |
Chromatography Type: | HILIC |