Summary of Study ST002783

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001736. The data can be accessed directly via it's Project DOI: 10.21228/M89Q7K This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002783
Study TitleMetabolic changes of the pig kidney during isolated normothermic perfusion with autologous whole blood - perfusate
Study TypeExperimental
Study SummaryThis study investigates how glucose, lactate and 20 amino acids in the perfusate of normothermically perfused pig kidneys changes over time. It also studies how type and severity of ischemia before perfusion (cold or warm ischemia) change this profile.
Institute
KU Leuven
DepartmentMicrobiology, Immunology and Transplantation
LaboratoryLab of Abdominal Transplantation
Last NameJochmans
First NameIna
AddressHerestraat 49, Leuven, 3000, Belgium
Emailina.jochmans@kuleuven.be
PhoneNone
Submit Date2023-07-17
Num Groups3
Total Subjects18 kidneys
Num Males9 male pigs
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2023-08-07
Release Version1
Ina Jochmans Ina Jochmans
https://dx.doi.org/10.21228/M89Q7K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO002883
Collection Summary:Perfusate samples were collected in ethylenediaminetetraacetic acid (EDTA) tubes, at baseline (before the kidney was mounted on the normothermic perfusion device), every 15 minutes during the first hour and hourly thereafter. One milliliter perfusate aliquots (centrifuged 1000g, 10 minutes, 4°C) were snap frozen in liquid nitrogen and stored at -80°C until analysis.
Sample Type:Perfusate
Collection Frequency:Baseline (T0) and during perfusion at 15 min (T0.25), 30 min (T0.50), 45 min (T0.75), 1 hour (T1), 2 hours (T2), 3 hours (T3), 4 hours (T4)
Storage Conditions:-80℃
Additives:EDTA
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