Summary of Study ST002783
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001736. The data can be accessed directly via it's Project DOI: 10.21228/M89Q7K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002783 |
Study Title | Metabolic changes of the pig kidney during isolated normothermic perfusion with autologous whole blood - perfusate |
Study Type | Experimental |
Study Summary | This study investigates how glucose, lactate and 20 amino acids in the perfusate of normothermically perfused pig kidneys changes over time. It also studies how type and severity of ischemia before perfusion (cold or warm ischemia) change this profile. |
Institute | KU Leuven |
Department | Microbiology, Immunology and Transplantation |
Laboratory | Lab of Abdominal Transplantation |
Last Name | Jochmans |
First Name | Ina |
Address | Herestraat 49, Leuven, 3000, Belgium |
ina.jochmans@kuleuven.be | |
Phone | None |
Submit Date | 2023-07-17 |
Num Groups | 3 |
Total Subjects | 18 kidneys |
Num Males | 9 male pigs |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2023-08-07 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002896 |
Sampleprep Summary: | Samples were extracted in an 80% methanol (80:20 methanol:water) (Methanol ≥99.9%, HiPerSolv CHROMANORM®, ULTRA for LC-MS, suitable for UPLC/UHPLC-MS instruments, VWR, Belgium) extraction buffer containing 1 µM of deuterated D27 myristic acid, 5 µM D12 glucose, 3 µM 13C5-D5-15N Glutamic acid and 3 µM D7-15N4-Arginine as internal standards. 10 µl of sample was added to 990 µl of the extraction buffer and stored overnight at -80 °C. Insolubilities and precipitated proteins were removed by centrifugation at 20.000 g, for 15 min at 4 °C. 200 µL of the supernatant was transferred to an appropriate mass-spectrometry vial. 10 µl of sample was added to 990 µl of the extraction buffer and stored overnight at -80 °C. |
Extract Storage: | -80℃ |