Summary of Study ST001944
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001231. The data can be accessed directly via it's Project DOI: 10.21228/M8KT3K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001944 |
Study Title | Growth-stage related diatom-bacteria interactions |
Study Summary | Phytoplankton-derived metabolites fuel a large fraction of heterotrophic bacterial production in the global ocean, yet methodological challenges have limited our knowledge of organic molecules transferred between these two microbial groups. In an experimental bloom study in which the diatom Thalassiosira pseudonana was co-cultured with three heterotrophic marine bacteria, we concurrently measured diatom endometabolites (i.e., potential exometabolite supply) by nuclear magnetic resonance (NMR) spectroscopy and bacterial gene expression (i.e., potential exometabolite uptake) by metatranscriptomic sequencing. |
Institute | University of Georgia |
Department | Department of Marine Sciences; Complex Carbohydrate Research Center |
Laboratory | Moran Lab, Edison Lab |
Last Name | Mario |
First Name | Uchimiya |
Address | 315 Riverbend Rd, Athens, GA 30602 |
mario.uchimiya@uga.edu | |
Phone | (706) 542-8387 |
Submit Date | 2021-08-31 |
Raw Data Available | Yes |
Raw Data File Type(s) | fid |
Analysis Type Detail | NMR |
Release Date | 2021-11-04 |
Release Version | 1 |
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Collection:
Collection ID: | CO002015 |
Collection Summary: | During this synthetic bloom experiment, axenic cultures of the diatom Thalssiosira pseudonana CCMP1335 (National Center for Marine Algae) were inoculated with equal cell numbers of the heterotrophic bacteria Ruegeria pomeroyi DSS-3 (Rhodobacterales), Stenotrophomonas sp. SKA-14 (Xanthomonadales), and Polaribacter dokdonensis MED-152 (Flavobacteriales) and co-cultured for 20 d. Co-cultured diatoms were harvested at day 3 and day 15, as well as axenic cultures (diatom only) day 15. Subsamples of 700-1000 ml were filtered onto 2.0 µm pore-size Isopore (Millipore, Burlington, MA) filters. Filters were stored in 50 ml falcon tubes at -80°C until processing. |
Collection Protocol Filename: | 2_Collection protocol_UGA_phytoplankton_ Aug2021.docx |
Collection Protocol Comments: | Details are in 2_Collection protocol_UGA_phytoplankton_ Aug2021.docx. |
Sample Type: | Algae |
Storage Conditions: | -80℃ |