Summary of Study ST001944
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001231. The data can be accessed directly via it's Project DOI: 10.21228/M8KT3K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001944 |
| Study Title | Growth-stage related diatom-bacteria interactions |
| Study Summary | Phytoplankton-derived metabolites fuel a large fraction of heterotrophic bacterial production in the global ocean, yet methodological challenges have limited our knowledge of organic molecules transferred between these two microbial groups. In an experimental bloom study in which the diatom Thalassiosira pseudonana was co-cultured with three heterotrophic marine bacteria, we concurrently measured diatom endometabolites (i.e., potential exometabolite supply) by nuclear magnetic resonance (NMR) spectroscopy and bacterial gene expression (i.e., potential exometabolite uptake) by metatranscriptomic sequencing. |
| Institute | University of Georgia |
| Department | Department of Marine Sciences; Complex Carbohydrate Research Center |
| Laboratory | Moran Lab, Edison Lab |
| Last Name | Mario |
| First Name | Uchimiya |
| Address | 315 Riverbend Rd, Athens, GA 30602 |
| mario.uchimiya@uga.edu | |
| Phone | (706) 542-8387 |
| Submit Date | 2021-08-31 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2021-11-04 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR002034 |
| Treatment Summary: | The diatom was grown in organic-carbon free L1 medium (Guillard and Hargraves 1993) prepared in acid washed glass containers at a salinity of 35 (Harrison et al. 1980) for one week prior to the start of the experiment. Cultures were grown with a 16:8 h light:dark cycle under 160 µmol photons m-2 s-1 at 18°C and checked for bacterial contamination by plating on rich medium (½ YTSS). On Day 0 of the experiment, diatoms were transferred into 1.9 L culture flasks containing 1000 ml of medium made with 13C-bicarbonate (final concentration of ~ 2x103 cells ml-1). One flask was kept as an L1 medium control without organisms. The three strains of heterotrophic bacteria were grown overnight in rich medium made with artificial seawater, either ½ YTSS medium (R. pomeroyi and Stenotrophomonas) or 1/10 YTSS medium (P. dokdonensis). Cells were harvested in exponential growth phase and washed five times in the same artificial sea water used for preparing the L1 medium. The bacteria were inoculated in equal proportions of OD600 into 15 flasks containing diatoms, with a final combined concentration of ca. 1x105 cells ml-1. One set of three flasks remained axenic. Three co-culture flasks were sacrificed at 4 pm days 3 and 15, and the axenic flasks day 15. |
| Treatment Protocol Filename: | 3_Treatment protocol_UGA_phytoplankton_ Aug2021.docx |
| Treatment Protocol Comments: | Details are in 3_Treatment protocol_UGA_phytoplankton_ Aug2021.docx. |
| Treatment: | Incubation of a diatom strain with and without bacterial strains |
