Summary of Study ST001944
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001231. The data can be accessed directly via it's Project DOI: 10.21228/M8KT3K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001944 |
Study Title | Growth-stage related diatom-bacteria interactions |
Study Summary | Phytoplankton-derived metabolites fuel a large fraction of heterotrophic bacterial production in the global ocean, yet methodological challenges have limited our knowledge of organic molecules transferred between these two microbial groups. In an experimental bloom study in which the diatom Thalassiosira pseudonana was co-cultured with three heterotrophic marine bacteria, we concurrently measured diatom endometabolites (i.e., potential exometabolite supply) by nuclear magnetic resonance (NMR) spectroscopy and bacterial gene expression (i.e., potential exometabolite uptake) by metatranscriptomic sequencing. |
Institute | University of Georgia |
Department | Department of Marine Sciences; Complex Carbohydrate Research Center |
Laboratory | Moran Lab, Edison Lab |
Last Name | Mario |
First Name | Uchimiya |
Address | 315 Riverbend Rd, Athens, GA 30602 |
mario.uchimiya@uga.edu | |
Phone | (706) 542-8387 |
Submit Date | 2021-08-31 |
Raw Data Available | Yes |
Raw Data File Type(s) | fid |
Analysis Type Detail | NMR |
Release Date | 2021-11-04 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
NMR:
NMR ID: | NM000216 |
Analysis ID: | AN003166 |
Instrument Name: | AVANCE Ⅲ |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 2D-1H-13C |
NMR Comments: | Details are in 5_Analysis protocol_UGA_phytoplankton_ Aug2021.docx. All the experiment and processing parameters are in 6_Acquisition and processing parameters_UGA_phytoplankton_ Aug2021.xlsx. |
Standard Concentration: | 1 mmol L-1 |
Spectrometer Frequency: | 600 MHz |
NMR Probe: | 5 mm TXI |
NMR Solvent: | Sodium phosphate buffer |
NMR Tube Size: | 5 mm |
Shimming Method: | topshim |
Pulse Sequence: | hsqcetgpprsisp2.2; hsqcdietgpsisp.2 |
Water Suppression: | Yes |
Chemical Shift Ref Cpd: | 2,2-dimethyl-2-silapentane-5-sulfonate-d6 |
Temperature: | 25 |