Summary of Study ST002522
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001624. The data can be accessed directly via it's Project DOI: 10.21228/M8S99J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002522 |
Study Title | Lipidomics study on the effect of LBP protein on hepatic lipid composition in mice |
Study Summary | Stress elevates the formation of ROS and lipid peroxidation, which induce lipid droplets (LDs) accumulation and adverse metabolic disturbance. Here, we explored the novel role of Lipopolysaccharide-binding protein (LBP) as an anti-oxidant, which can capture unsaturated triglyceride (TG) into LDs to avoid lipid peroxidation. Oxidative stress upregulates LBP level and promotes LDs growth via the LBP/TG phase transition. Upon N-Acetyl-L-cysteine (NAC) elimination of ROS, LBP is exported from LD along with PRDX4, resulting in an increase in phospholipid synthesis. Chronic stress causes LBP upregulation and leads to obesity, which can be rescued by NAC treatment in vivo. These results support that LBP maintains homeostasis by coupling lipid metabolism and redox signal, which provides insights into redox medicine that mitigate stress-induced metabolic dysfunction. Hepatic lipidomics in overexpressed LBP and WT mice treated with NAC after 24h fasting |
Institute | University of Science and Technology of China |
Department | Department of Endocrinology and Laboratory for Diabetes |
Laboratory | The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine |
Last Name | Zhang |
First Name | Qilun |
Address | Lujiang road no.17 |
zql66666@mail.ustc.edu.cn | |
Phone | +8618356507293 |
Submit Date | 2023-03-21 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2024-03-21 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004155 | AN004156 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | SHIMADZU UHPLC Nexera LC-30A | SHIMADZU UHPLC Nexera LC-30A |
Column | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Relative intensity | Relative intensity |
MS:
MS ID: | MS003902 |
Analysis ID: | AN004155 |
Instrument Name: | Thermo Q Exactive Plus Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | mass spectrometric analysis was performed using a Q Exactive mass spectrometer (Thermo Scientific). ESI source conditions were as follows: Heater Temp 300 °C, Sheath Gas Flow rate 45 arb, Aux Gas Flow Rate15 arb, Sweep Gas Flow Rate 1arb, Spray voltage 3.0KV, Capillary Temp 350 °C, S-Lens RF Level 50%, MS1scan ranges: 200-1800. The mass-charge ratio of lipid molecules and lipid fragments were obtained by collecting 10 fragment maps (MS 2scan, HCD) after each fullscan. The resolution of MS1 at M/Z 200 was 70,000 and that of MS2 at M/Z 200 was 17,500. LipidSearch was used for peak identification, peak extraction and lipid molecules identification (secondary identification). The main parameters were precursor tolerance: 5 ppm, Product Tolerance: 5 ppm, and Product Ion Threshold: 5%. The obtained data were subjected to quality control for subsequent data lipid difference analysis. |
Ion Mode: | POSITIVE |
MS ID: | MS003903 |
Analysis ID: | AN004156 |
Instrument Name: | Thermo Q Exactive Plus Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | mass spectrometric analysis was performed using a Q Exactive mass spectrometer (Thermo Scientific). ESI source conditions were as follows: Heater Temp 300 °C, Sheath Gas Flow rate 45 arb, Aux Gas Flow Rate15 arb, Sweep Gas Flow Rate 1arb, Spray voltage 3.0KV, Capillary Temp 350 °C, S-Lens RF Level 50%, MS1scan ranges: 200-1800. The mass-charge ratio of lipid molecules and lipid fragments were obtained by collecting 10 fragment maps (MS 2scan, HCD) after each fullscan. The resolution of MS1 at M/Z 200 was 70,000 and that of MS2 at M/Z 200 was 17,500. LipidSearch was used for peak identification, peak extraction and lipid molecules identification (secondary identification). The main parameters were precursor tolerance: 5 ppm, Product Tolerance: 5 ppm, and Product Ion Threshold: 5%. The obtained data were subjected to quality control for subsequent data lipid difference analysis. |
Ion Mode: | NEGATIVE |