Summary of Study ST002522

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001624. The data can be accessed directly via it's Project DOI: 10.21228/M8S99J This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002522
Study Title	Lipidomics study on the effect of LBP protein on hepatic lipid composition in mice
Study Summary	Stress elevates the formation of ROS and lipid peroxidation, which induce lipid droplets (LDs) accumulation and adverse metabolic disturbance. Here, we explored the novel role of Lipopolysaccharide-binding protein (LBP) as an anti-oxidant, which can capture unsaturated triglyceride (TG) into LDs to avoid lipid peroxidation. Oxidative stress upregulates LBP level and promotes LDs growth via the LBP/TG phase transition. Upon N-Acetyl-L-cysteine (NAC) elimination of ROS, LBP is exported from LD along with PRDX4, resulting in an increase in phospholipid synthesis. Chronic stress causes LBP upregulation and leads to obesity, which can be rescued by NAC treatment in vivo. These results support that LBP maintains homeostasis by coupling lipid metabolism and redox signal, which provides insights into redox medicine that mitigate stress-induced metabolic dysfunction. Hepatic lipidomics in overexpressed LBP and WT mice treated with NAC after 24h fasting
Institute	University of Science and Technology of China
Department	Department of Endocrinology and Laboratory for Diabetes
Laboratory	The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine
Last Name	Zhang
First Name	Qilun
Address	Lujiang road no.17
Email	zql66666@mail.ustc.edu.cn
Phone	+8618356507293
Submit Date	2023-03-21
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2024-03-21
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002628
Sampleprep Summary:	Lipids were extracted according to MTBE method. Briefly, a 200-µL volume of water was added to 30 mg sample and vortexed for 5 s. Subsequently, 240 µL of precooling methanol was added and the mixture vortexed for 30 s. After that, 800 µL of MTBE was added and the mixture was ultrasound 20 min at 4℃ followed by sitting still for 30 min at room temperature. The solution was centrifuged at 14000g for 15min at 10℃ and the upper organic solvent layer was obtained and dried under nitrogen.

Sampleprep ID:

SP002628

Sampleprep Summary:

Lipids were extracted according to MTBE method. Briefly, a 200-µL volume of water was added to 30 mg sample and vortexed for 5 s. Subsequently, 240 µL of precooling methanol was added and the mixture vortexed for 30 s. After that, 800 µL of MTBE was added and the mixture was ultrasound 20 min at 4℃ followed by sitting still for 30 min at room temperature. The solution was centrifuged at 14000g for 15min at 10℃ and the upper organic solvent layer was obtained and dried under nitrogen.