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MB Sample ID: SA008211
| Local Sample ID: | P1T |
| Subject ID: | SU000169 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 63|27|23|43 |
| Gender: | Male|Female|Female|Male |
| Human Race: | African American|African American|White, non-hispanic|African American |
| Human Ethnicity: | Cerebral palsy, mental retardation, scoliosis, seizure disorder|Deep vein thrombosis, anemia, bipolar, herpes simplex virus|Urinary tract infection, anxiety, depression |
| Human Lifestyle Factors: | None|Cerebral palsy, mental retardation, scoliosis, seizure disorder|Deep vein thrombosis, anemia, bipolar, herpes simplex virus|Urinary tract infection, anxiety, depression |
| Human Medications: | Systemic:Bactrim Topical:None|None|Systemic:Vancomycin|Systemic:Doxycycline Topical:Mesalt |
| Human Nutrition: | Well nourished|G-tube fed, liquid only, malnourished|Well nourished|Inadequate protein intake |
| Species Group: | Human |
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Subject:
| Subject ID: | SU000169 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 63|27|23|43 |
| Gender: | Male|Female|Female|Male |
| Human Race: | African American|African American|White, non-hispanic|African American |
| Human Ethnicity: | Cerebral palsy, mental retardation, scoliosis, seizure disorder|Deep vein thrombosis, anemia, bipolar, herpes simplex virus|Urinary tract infection, anxiety, depression |
| Human Lifestyle Factors: | None|Cerebral palsy, mental retardation, scoliosis, seizure disorder|Deep vein thrombosis, anemia, bipolar, herpes simplex virus|Urinary tract infection, anxiety, depression |
| Human Medications: | Systemic:Bactrim Topical:None|None|Systemic:Vancomycin|Systemic:Doxycycline Topical:Mesalt |
| Human Nutrition: | Well nourished|G-tube fed, liquid only, malnourished|Well nourished|Inadequate protein intake |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| P1T | SA008211 | FL001973 | Patient 1 | Patient |
Collection:
| Collection ID: | CO000155 |
| Collection Summary: | Samples were collected via punch biopsy from wound edge and sectioned into top and bottom |
| Collection Protocol Comments: | Chronicity is defined as wounds lasting more than 30 days |
| Sample Type: | Tissue |
| Collection Method: | Punch biopsy |
| Collection Location: | Wound Edge |
| Collection Frequency: | Single Collection |
| Volumeoramount Collected: | 9-32 mg |
| Storage Conditions: | Flash frozen in liquid nitrogen, shipped on dry ice, stored at -80C |
| Tissue Cell Identification: | T=Top section B=Bottom section |
| Tissue Cell Quantity Taken: | 9-32mg |
Treatment:
| Treatment ID: | TR000174 |
Sample Preparation:
| Sampleprep ID: | SP000169 |
| Sampleprep Summary: | Cold methanol/chloroform extraction |
| Processing Method: | Biopsy samples were resuspended in ice-cold 60% aqueous methanol and homogenized using a tissue homogenizer (Tissue Tearor™ Model 985370-395, Biospec Products Inc., Bartlesville, OK) set to 2-minute intervals of 10 seconds on and 5 seconds off |
| Processing Storage Conditions: | On ice |
| Extraction Method: | 1:1 aqueous chloroform |
| Extract Cleanup: | Aqueous layers collected and lyophilized |
| Extract Storage: | Lyophilized samples were stored at -80C |
| Sample Resuspension: | Resuspended in 10mM NaH2PO4/Na2HPO4 |
| Organ: | Skin |
| Organ Specification: | Chronic wound biopsy |
Analysis:
| MB Sample ID: | SA008211 |
| Analysis ID: | AN000237 |
| Laboratory Name: | Copie Lab |
| Analysis Type: | NMR |
| Software Version: | Topspin version 3 |
| Operator Name: | Brian Tripet |
| Detector Type: | cryoprobeTM |
| Chromatography ID: | CH000166 |
| Num Factors: | 4 |
| Num Metabolites: | 121 |
NMR:
| NMR ID: | NM000052 |
| Analysis ID: | AN000237 |
| Instrument Name: | Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Spectrometer Frequency: | 600 MHz |
| Offset Frequency: | 600 mHz |
| Presaturation Power Level: | cryoprobeTM |
| Chemical Shift Ref Cpd: | 10mM NaH2PO4/ Na2HPO4 |
