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MB Sample ID: SA008211
| Local Sample ID: | P1T |
| Subject ID: | SU000169 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 63|27|23|43 |
| Gender: | Male|Female|Female|Male |
| Human Race: | African American|African American|White, non-hispanic|African American |
| Human Ethnicity: | Cerebral palsy, mental retardation, scoliosis, seizure disorder|Deep vein thrombosis, anemia, bipolar, herpes simplex virus|Urinary tract infection, anxiety, depression |
| Human Lifestyle Factors: | None|Cerebral palsy, mental retardation, scoliosis, seizure disorder|Deep vein thrombosis, anemia, bipolar, herpes simplex virus|Urinary tract infection, anxiety, depression |
| Human Medications: | Systemic:Bactrim Topical:None|None|Systemic:Vancomycin|Systemic:Doxycycline Topical:Mesalt |
| Human Nutrition: | Well nourished|G-tube fed, liquid only, malnourished|Well nourished|Inadequate protein intake |
| Species Group: | Human |
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Sample Preparation:
| Sampleprep ID: | SP000169 |
| Sampleprep Summary: | Cold methanol/chloroform extraction |
| Processing Method: | Biopsy samples were resuspended in ice-cold 60% aqueous methanol and homogenized using a tissue homogenizer (Tissue Tearor™ Model 985370-395, Biospec Products Inc., Bartlesville, OK) set to 2-minute intervals of 10 seconds on and 5 seconds off |
| Processing Storage Conditions: | On ice |
| Extraction Method: | 1:1 aqueous chloroform |
| Extract Cleanup: | Aqueous layers collected and lyophilized |
| Extract Storage: | Lyophilized samples were stored at -80C |
| Sample Resuspension: | Resuspended in 10mM NaH2PO4/Na2HPO4 |
| Organ: | Skin |
| Organ Specification: | Chronic wound biopsy |
