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MB Sample ID: SA015049
| Local Sample ID: | 121079 |
| Subject ID: | SU000357 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | Not applicable |
| Age Or Age Range: | Variable |
| Weight Or Weight Range: | Variable |
| Height Or Height Range: | Variable |
| Gender: | Variable |
| Human Race: | Variable |
| Human Ethnicity: | Variable |
| Human Trial Type: | Not applicable |
| Human Lifestyle Factors: | Not applicable |
| Human Medications: | Not applicable |
| Human Prescription Otc: | Not applicable |
| Human Smoking Status: | Not applicable |
| Human Alcohol Drug Use: | Not applicable |
| Human Nutrition: | Not applicable |
| Human Inclusion Criteria: | Deceased organ donor with consent for use of tissue for research |
| Human Exclusion Criteria: | None |
| Animal Animal Supplier: | in house/in house |
| Subject Comments: | Liver biopsy is routinely performed on livers used for solid organ transplantation. The tissue is from decedent subjects. Consent for use of tissue is obtained at the time of organ donation by the local organ procurement organization. |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000357 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | Not applicable |
| Age Or Age Range: | Variable |
| Weight Or Weight Range: | Variable |
| Height Or Height Range: | Variable |
| Gender: | Variable |
| Human Race: | Variable |
| Human Ethnicity: | Variable |
| Human Trial Type: | Not applicable |
| Human Lifestyle Factors: | Not applicable |
| Human Medications: | Not applicable |
| Human Prescription Otc: | Not applicable |
| Human Smoking Status: | Not applicable |
| Human Alcohol Drug Use: | Not applicable |
| Human Nutrition: | Not applicable |
| Human Inclusion Criteria: | Deceased organ donor with consent for use of tissue for research |
| Human Exclusion Criteria: | None |
| Animal Animal Supplier: | in house/in house |
| Subject Comments: | Liver biopsy is routinely performed on livers used for solid organ transplantation. The tissue is from decedent subjects. Consent for use of tissue is obtained at the time of organ donation by the local organ procurement organization. |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 121079 | SA015049 | FL003360 | Anoxia | DONOR CAUSE OF DEATH |
Collection:
| Collection ID: | CO000351 |
| Collection Summary: | A wedge biopsy of the right lobe of the liver is routinely obtained prior to transplantation. The sample is immediately flash frozen in the operating room and subsequently transferred to Ochsner Biobank for sample storage. |
| Sample Type: | Liver Tissue |
| Collection Method: | Open biopsy during cold storage preservation of organ for transplant |
| Collection Location: | Operating room of liver transplant during |
| Collection Frequency: | Single sample prior to transplant |
| Collection Duration: | Not applicable |
| Collection Time: | Not applicable |
| Volumeoramount Collected: | ~50mg |
| Storage Conditions: | -180 degree C, liquid nitrogen vapor phase |
| Collection Vials: | ThermoScientific CryoTube vials, 1.8mL |
| Storage Vials: | ThermoScientific CryoTube vials, 1.8mL |
| Collection Tube Temp: | -70 degree liguid nitrogen flash freezing |
| Additives: | None |
| Blood Serum Or Plasma: | N/A |
| Tissue Cell Identification: | N/A |
| Tissue Cell Quantity Taken: | N/A |
Treatment:
| Treatment ID: | TR000371 |
| Treatment Summary: | Not Applicable |
| Treatment Protocol ID: | Not Applicable |
| Treatment Protocol Filename: | Not Applicable |
| Treatment Protocol Comments: | Not Applicable |
| Treatment: | Not Applicable |
| Treatment Compound: | Not Applicable |
| Treatment Route: | Not Applicable |
| Treatment Dose: | Not Applicable |
| Treatment Dosevolume: | Not Applicable |
| Treatment Doseduration: | Not Applicable |
| Treatment Vehicle: | Not Applicable |
Sample Preparation:
| Sampleprep ID: | SP001183 |
| Sampleprep Summary: | Amino acid assay|CoA assay|NAD-ATP assay|NADH assay|Organic acid assay |
| Sampleprep Protocol Filename: | SB_AA_Assay.pdf SB_CoA_Assay.pdf SB_NAD-ATP_Assay_Agilent.pdf SB_NADH_Assay_Agilent.pdf SB_OA_Assay.pdf |
Combined analysis:
| Analysis ID | AN000544 | AN000545 | AN000546 | AN000547 | AN000548 |
|---|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity | Agilent 1290 Infinity | Agilent 1290 Infinity | Thermo Dionex Ultimate 3000 |
| Column | Waters AccQ Tag (100 x 2mm,1.7um) | Waters Acquity BEH C18 (50 x 2mm,1.7um) | Thermo Hypercarb (50 x 2.1mm,3um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| MS Type | ESI | ESI | ESI | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 6490 QQQ | Agilent 6490 QQQ | Agilent 6490 QQQ | Agilent 6490 QQQ | Thermo Quantiva QQQ |
| Ion Mode | POSITIVE | POSITIVE | POSITIVE | POSITIVE | POSITIVE |
| Units | nmol/mg dry powder | pmol/mg dry powder | nmol/mg dry powder | nmol/mg dry powder | nmol/mg dry powder |
Chromatography:
| Chromatography ID: | CH000381 |
| Chromatography Summary: | Amino acid assay |
| Methods Filename: | SB_AA_Assay.pdf |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters AccQ Tag (100 x 2mm,1.7um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.7 mL/min |
| Solvent A: | AccQ·Tag Eluent A |
| Solvent B: | accQ·Tag Eluent B |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000382 |
| Chromatography Summary: | CoA assay |
| Methods Filename: | SB_CoA_Assay.pdf |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters Acquity BEH C18 (50 x 2mm,1.7um) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000383 |
| Chromatography Summary: | NAD-ATP assay |
| Methods Filename: | SB_NAD-ATP_Assay_Agilent.pdf |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Thermo Hypercarb (50 x 2.1mm,3um) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000384 |
| Chromatography Summary: | NADH assay |
| Methods Filename: | SB_NADH_Assay_Agilent.pdf |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 30 |
| Flow Rate: | 0.54 mL/min |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 6 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000385 |
| Chromatography Summary: | Organic acid assay |
| Methods Filename: | SB_OA_Assay.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Column Temperature: | 45 |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000480 |
| Analysis ID: | AN000544 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Amino acid assay- ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000481 |
| Analysis ID: | AN000545 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | CoA assay-ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000482 |
| Analysis ID: | AN000546 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | NAD-ATP assay-ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000483 |
| Analysis ID: | AN000547 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | NADH assay-ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000484 |
| Analysis ID: | AN000548 |
| Instrument Name: | Thermo Quantiva QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Organic acid assay-ESI |
| Ion Mode: | POSITIVE |
