Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA022622

Local Sample ID:	WB2-2
Subject ID:	SU000465
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Human

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Subject:

Subject ID:	SU000465
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Human

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
WB2-2	SA022622	FL005549	Control	Sample Treatment

Collection:

Collection ID:	CO000459
Collection Summary:	Freshly collected human urine
Sample Type:	Urine

Treatment:

Treatment ID:	TR000479
Treatment Summary:	No treatment was applied

Sample Preparation:

Sampleprep ID:	SP000472
Sampleprep Summary:	Bursting bubbles were generated at ambient conditions by feeding gas (1-100 kPa; 1-100 L/s) through an air diffuser at the bottom of diluted (200 times in ultrapure water) urine solution (50-500 mL). Diffusers with three different pore sizes were tested: 100 µm; 1-10 µm; 0.2-5 µm. The first diffuser was made in house by pulling 10 fused silica capillaries with i.d. 100 µm through a rubber piece. The second diffuser was made from porous ceramics and was purchased from Shiyuan Appliances, Shenzhongshan, Guangdong, China. The third diffuser was made from porous ceramics and was purchased from Junfeng Tradnig Ltd., Ningbo, Zhejiang, China. The bursting bubble aerosol was collected using a slanted glass slide fixed above the liquid surface. Typically, ca. 100 µL of aerosol was collected within 5-10 min from 500 mL bulk solution at the gas pressure of 10 kPa.

Sampleprep ID:

SP000472

Sampleprep Summary:

Bursting bubbles were generated at ambient conditions by feeding gas (1-100 kPa; 1-100 L/s) through an air diffuser at the bottom of diluted (200 times in ultrapure water) urine solution (50-500 mL). Diffusers with three different pore sizes were tested: 100 µm; 1-10 µm; 0.2-5 µm. The first diffuser was made in house by pulling 10 fused silica capillaries with i.d. 100 µm through a rubber piece. The second diffuser was made from porous ceramics and was purchased from Shiyuan Appliances, Shenzhongshan, Guangdong, China. The third diffuser was made from porous ceramics and was purchased from Junfeng Tradnig Ltd., Ningbo, Zhejiang, China. The bursting bubble aerosol was collected using a slanted glass slide fixed above the liquid surface. Typically, ca. 100 µL of aerosol was collected within 5-10 min from 500 mL bulk solution at the gas pressure of 10 kPa.

Combined analysis:

Analysis ID	AN000695
Analysis type	MS
Chromatography type	Reversed phase
Chromatography system	Agilent 1100
Column	homemade capillary (75ul id,12 cm,Reprosil-Pur Basic C18,3um,100 Å; Dr. Maisch HPLC GmbH,Germany)
MS Type	ESI
MS instrument type	LTQ-FT
MS instrument name	Thermo LTQ-FT Ultra
Ion Mode	POSITIVE
Units	Arbitrary Units

Chromatography:

Chromatography ID:	CH000503
Chromatography Summary:	Chromatographic separations were performed on an nano-HPLC Agilent 1100 system (Agilent Technologies) equipped with a homemade capillary column (75 µl id, 12 cm, Reprosil-Pur Basic C18, 3 µm, 100 Å; Dr. Maisch HPLC GmbH, Germany) applying the following binary gradient at a flow rate of 0.3 µl/min: 0.1% formic acid in water (v/v, solvent A) and 0.1% formic acid in acetonitrile (v/v, solvent B), from 3% to 50% (v/v) of solvent B over 90 min followed by isocratic elution (95%, v/v, of solvent B) for 15 min. The injection volume was 1.0 µl (partial loop injection).
Instrument Name:	Agilent 1100
Column Name:	homemade capillary (75ul id,12 cm,Reprosil-Pur Basic C18,3um,100 Å; Dr. Maisch HPLC GmbH,Germany)
Column Temperature:	Ambient
Flow Rate:	0.3 µl/min
Solvent A:	100% water; 0.1% formic acid
Solvent B:	100% acetonitrile; 0.1% formic acid
Chromatography Type:	Reversed phase

MS:

MS ID:	MS000617
Analysis ID:	AN000695
Instrument Name:	Thermo LTQ-FT Ultra
Instrument Type:	LTQ-FT
MS Type:	ESI
Ion Mode:	POSITIVE