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MB Sample ID: SA022622
| Local Sample ID: | WB2-2 |
| Subject ID: | SU000465 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
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Combined analysis:
| Analysis ID | AN000695 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1100 |
| Column | homemade capillary (75ul id,12 cm,Reprosil-Pur Basic C18,3um,100 Å; Dr. Maisch HPLC GmbH,Germany) |
| MS Type | ESI |
| MS instrument type | LTQ-FT |
| MS instrument name | Thermo LTQ-FT Ultra |
| Ion Mode | POSITIVE |
| Units | Arbitrary Units |
Chromatography:
| Chromatography ID: | CH000503 |
| Chromatography Summary: | Chromatographic separations were performed on an nano-HPLC Agilent 1100 system (Agilent Technologies) equipped with a homemade capillary column (75 µl id, 12 cm, Reprosil-Pur Basic C18, 3 µm, 100 Å; Dr. Maisch HPLC GmbH, Germany) applying the following binary gradient at a flow rate of 0.3 µl/min: 0.1% formic acid in water (v/v, solvent A) and 0.1% formic acid in acetonitrile (v/v, solvent B), from 3% to 50% (v/v) of solvent B over 90 min followed by isocratic elution (95%, v/v, of solvent B) for 15 min. The injection volume was 1.0 µl (partial loop injection). |
| Instrument Name: | Agilent 1100 |
| Column Name: | homemade capillary (75ul id,12 cm,Reprosil-Pur Basic C18,3um,100 Å; Dr. Maisch HPLC GmbH,Germany) |
| Column Temperature: | Ambient |
| Flow Rate: | 0.3 µl/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
