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MB Sample ID: SA078103
| Local Sample ID: | TP3_007 |
| Subject ID: | SU001192 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001192 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| TP3_007 | SA078103 | FL011904 | Trauma | Treatment |
Collection:
| Collection ID: | CO001186 |
| Collection Summary: | Serum samples were collected from each human after exposure to each treatment group. Samples were snap frozen and shipped to the WCMC on dry ice. Upon arrival, samples were placed in a -80°C freezer. |
| Sample Type: | Blood (serum) |
Treatment:
| Treatment ID: | TR001207 |
| Treatment Summary: | Samples were collected from patients who had undergone some type of physical trauma. This group consisted of six patients. Serum samples from six patients who had not experienced physical trauma, were considered health, and were of similar characteristics to the patients in the trauma group. Overall, 12 samples were collected from 2 groups of patients, comprised of six patients each. |
Sample Preparation:
| Sampleprep ID: | SP001200 |
| Sampleprep Summary: | All extraction procedures are performed over crushed ice. Samples were defrosted over crushed ice for 30 minutes or until defrosted. 30 µL of serum was aliquoted from the original sample into a 1.5 mL Eppendorf tube. 1 mL of 3:3:2 ACN:IPA:Water was added to each sample. Samples were vortexed for 10 seconds. Samples were shaken for 6 minutes at 4°C using an orbital mixing plate. Samples were centrifuged for 2 minutes at 14,000 rcf. 2 aliquots of 475 µL were removed from the supernatant and placed into new 1.5 mL Eppendorf tubes. 1 sample is frozen at -20°C and saved as a backup. The other sample is dried to completeness using a Labconco Centrivap. |
| Sample Derivatization: | To the dried down samples, 10 µL of a prepared methoxyamine solution was added to each sample. Samples were shaken on an Orbital Mixing Chilling/Heating Plate at 30°C for 1.5 hours. 91 µL of a N-methyl-N-(trimethylsilyl)-trifluoroacetamide (MSTFA) and fatty-acid methyl-esters (FAMES) solution was added to each sample. Samples were shaken on an Orbital Mixing Chilling/Heating Plate at 37°C for 30 minutes. Contents were transferred to individual glass vials with micro-inserts and capped immediately. Submitted to GC-MS analysis. |
Combined analysis:
| Analysis ID | AN001856 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890A |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus III GC TOF |
| Ion Mode | POSITIVE |
| Units | Peak Height |
Chromatography:
| Chromatography ID: | CH001343 |
| Instrument Name: | Agilent 7890A |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Column Temperature: | 50-330°C |
| Flow Rate: | 1 mL min^(-1) |
| Injection Temperature: | 50°C ramped to 250°C by 12°C s-1 |
| Oven Temperature: | 50°C for 1 min, then ramped at 20°C min-1 to 330°C, held constant for 5 min |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001716 |
| Analysis ID: | AN001856 |
| Instrument Name: | Leco Pegasus III GC TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| MS Comments: | N/A |
| Ion Mode: | POSITIVE |
| Ion Source Temperature: | 250°C |
| Ionization Energy: | -70 eV |
| Resolution Setting: | 17 spectra s-1 |
