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MB Sample ID: SA094274
| Local Sample ID: | HC-HMA-Rag1KO+Ctrl Ab #6 |
| Subject ID: | SU001375 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001375 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| HC-HMA-Rag1KO+Ctrl Ab #6 | SA094274 | FL013473 | Control antibody | Factor |
Collection:
| Collection ID: | CO001370 |
| Collection Summary: | Feces of anti-IL-22 antibody/control antibody-treated HC-HMA mice were harvested. |
| Sample Type: | Feces |
Treatment:
| Treatment ID: | TR001390 |
| Treatment Summary: | GF C57BL/6 mice were colonized with HC microbiotas for 2 weeks. After colonization of HC microbiota, the mice were treated with control or anti-IL-22 antibody twice (day -5 and day -3) before collecting fecal samples. |
Sample Preparation:
| Sampleprep ID: | SP001383 |
| Sampleprep Summary: | Fecal samples were lyophilized using a VD-800R lyophilizer (TAITEC) for 24 hours. Freeze-dried feces were disrupted with 3.0-mm Zirconia Beads (Biomedical Science) by vigorous shaking (1,500 rpm for 10 min) using Shake Master (Biomedical Science). Fecal metabolites were extracted using the methanol:chloroform:water extraction protocol. |
Combined analysis:
| Analysis ID | AN002166 | AN002167 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | CE | CE |
| Chromatography system | Agilent 7100 CE | Agilent 7100 CE |
| Column | Fused-silica,50um(cation) | COSMO(+),50um(anion |
| MS Type | ESI | ESI |
| MS instrument type | Other | Other |
| MS instrument name | Agilent G3250AA LC/MSD TOF system | Agilent G3250AA LC/MSD TOF system |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | nmol/g | nmol/g |
Chromatography:
| Chromatography ID: | CH001584 |
| Instrument Name: | Agilent 7100 CE |
| Column Name: | Fused-silica,50um(cation) |
| Internal Standard: | 20 μM of methionine sulfone |
| Chromatography Type: | CE |
| Chromatography ID: | CH001585 |
| Instrument Name: | Agilent 7100 CE |
| Column Name: | COSMO(+),50um(anion |
| Internal Standard: | 20 μM of D-camphor-10-sulfonic acid (CSA) |
| Chromatography Type: | CE |
MS:
| MS ID: | MS002015 |
| Analysis ID: | AN002166 |
| Instrument Name: | Agilent G3250AA LC/MSD TOF system |
| Instrument Type: | Other |
| MS Type: | ESI |
| MS Comments: | In-house software (MasterHands) was used for peak area integration and annotation. |
| Ion Mode: | POSITIVE |
| MS ID: | MS002016 |
| Analysis ID: | AN002167 |
| Instrument Name: | Agilent G3250AA LC/MSD TOF system |
| Instrument Type: | Other |
| MS Type: | ESI |
| MS Comments: | In-house software (MasterHands) was used for peak area integration and annotation. |
| Ion Mode: | NEGATIVE |
