Return to study ST001863 main page
MB Sample ID: SA174440
| Local Sample ID: | HT29_con1 |
| Subject ID: | SU001940 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Biosource Or Supplier: | ATCC |
| Cell Strain Details: | HT-29 and Caco-2 cells |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001940 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Biosource Or Supplier: | ATCC |
| Cell Strain Details: | HT-29 and Caco-2 cells |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| HT29_con1 | SA174440 | FL021427 | Control | Treatment |
Collection:
| Collection ID: | CO001933 |
| Collection Summary: | Cell supernatants were collected by centrifugation of the epithelial cells and frozen at -80 degrees until analysis. |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR001952 |
| Treatment Summary: | Cells were treated with 200nM Bxa protein and compared to a control of the same buffer but without protein. |
Sample Preparation:
| Sampleprep ID: | SP001946 |
| Sampleprep Summary: | Samples were prepped by adding isopropanol to the supernatant to extract metabolites and then injected on the machine |
Combined analysis:
| Analysis ID | AN003020 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | ID-X |
| Column | C8 |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | Area under curve |
Chromatography:
| Chromatography ID: | CH002238 |
| Instrument Name: | ID-X |
| Column Name: | C8 |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS002809 |
| Analysis ID: | AN003020 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Data were analyzed using Progenesis QI peak picking software ThermoScientific Trace Finder 5.0. |
| Ion Mode: | POSITIVE |
