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MB Sample ID: SA004272

Local Sample ID:SBEP_Microbiome.022
Subject ID:SU000102
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:129/SvJ
Age Or Age Range:6- to 8-week-old
Animal Animal Supplier:Jackson Laboratories,Bar Harbor, ME
Animal Housing:specific pathogen-free conditions in filter-top cages
Animal Feed:food provided ad libitum
Animal Water:sterile water
Species Group:Mammal

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Combined analysis:

Analysis ID AN000135
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890A
Column Agilent HP5-MS (30m × 0.25mm, 0.25 um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5975C
Units Peak area


Chromatography ID:CH000095
Chromatography Summary:Agilent 7890A gas chromatograph with a HP-5MS gas chromatography column using Chemstation
Chromatography Comments:Chromatography was carried out on an Agilent 7890A gas chromatograph using the manufacturer's software (Chemstation) and a HP-5MS gas chromatography column (Agilent Technologies, Santa Clara, CA; 30 m x 0.25 mm x 0.25 m film thickness). The sample injection mode was splitless, and 1 L of each sample was injected. The injection port temperature was held at 250 C throughout the analysis. The GC oven was held at 60 C for 1 min after injection, and the temperature was then increased to 325 C by 10 C/min, followed by a 5 min hold at 325 C. The helium gas flow rates for each Experiment were determined by the Agilent Retention Time Locking function based on analysis of deuterated myristic acid and were in the range of 0.450.5 mL/min.
Instrument Name:Agilent 7890A
Column Name:Agilent HP5-MS (30m × 0.25mm, 0.25 um)
Flow Rate:0.450.5 mL/min
Injection Temperature:250 C
Sample Injection:1 L, splitless
Analytical Time:37.5 min
Oven Temperature:60 C for 1 min, then increased to 325 C by 10 C/min, followed by a 5 min hold at 325 C
Sample Syringe Size:10 L
Chromatography Type:GC